Experimental induction of blue-sac disease

Blue‐sac disease, an abnormal condition of sac fry, has been recognized for nearly 90 years. At least 23 postulated causes have been advanced, but reports of consistently reproducible results have not been found in literature. Methods whereby blue‐sac disease was induced at will are described. Closed‐system incubation, whereby metabolic wastes accumulated, resulted in incidences of blue‐sac disease roughly proportional to the period of holding and to ammonia concentrations which developed. Blue‐sac disease was also induced by adding synthetic nitrogen compounds to water used for incubating eggs and fry.