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Extraction and analysis of adenosine triphosphate from aquatic environments

January 1, 1994

A variety of adenosine triphosphate (ATP) extraction procedures have been investigated for their applicability to samples from aquatic environments. The cold sulfuric-oxalic acid procedure was best suited to samples consisting of water, periphyton, and sediments. Due to cation and fulvic acid interferences, a spike with a known quantity of ATP was necessary to estimate losses when sediments were extracted. Variable colonization densities for periphyton required that several replicates be extracted to characterize acdurately the periphyton community. Extracted samples were stable at room temperature for one to five hours, depending on the ATP concentration, if the pH was below 2. Neutralized samples which were quick frozen and stored at -30°C were stable for months.

Citation Information

Publication Year 1981
Title Extraction and analysis of adenosine triphosphate from aquatic environments
DOI 10.3133/wri815
Authors Doyle W. Stephens, David J. Shultz
Publication Type Report
Publication Subtype USGS Numbered Series
Series Title Water-Resources Investigations Report
Series Number 81-5
Index ID wri815
Record Source USGS Publications Warehouse
USGS Organization