Strip‐spawning trials were performed with spotted seatrout (Cynoscion nebulosus) after females received injections of 250, 500, and 1,100 IU human chorionic gonadotropin (HCG)/kg body weight (BW); 10, 50, and 100 mg carp pituitary extract/kg BW; 10, 50, and 100 mg salmon pituitary extract/kg BW; and a crude spotted seatrout pituitary extract administered on the basis of two, four, and six pituitaries per fish. Each hormone dose was administered to three females on each of four trial dates. All hormone types induced ovulation. The percentage of fish induced to ovulate by each hormone generally increased with dosage. Only HCG administered at 1,100 IU/kg BW caused ovulation in all fish treated. The different hormone preparations did not significantly affect fertilization once ovulation occurred (P > 0.05).