Two polymorphic, duplicated loci (fumarase-1,2 and phosphoglucomutase-3,4), previously undescribed in salmonids, were examined in lake trout to determine their electrophoretic expression, mode of inheritance, and linkage associations. Both loci appear to be located on chromosomes which show residual tetrasomy. Neither locus pair shows joint segregation with other loci examined or with each other.