Molecular testing of adult Pacific salmon and trout (Oncorhynchus spp.) for several RNA viruses demonstrates widespread distribution of piscine orthoreovirus in Alaska and Washington
This research was initiated in conjunction with a systematic, multiagency surveillance effort in the United States (U.S.) in response to reported findings of infectious salmon anaemia virus (ISAV) RNA in British Columbia, Canada. In the systematic surveillance study reported in a companion paper, tissues from various salmonids taken from Washington and Alaska were surveyed for ISAV RNA using the U.S.-approved diagnostic method, and samples were released for use in this present study only after testing negative. Here, we tested a subset of these samples for ISAV RNA with three additional published molecular assays, as well as for RNA from salmonid alphavirus (SAV), piscine myocarditis virus (PMCV) and piscine orthoreovirus (PRV). All samples (n = 2,252; 121 stock cohorts) tested negative for RNA from ISAV, PMCV, and SAV. In contrast, there were 25 stock cohorts from Washington and Alaska that had one or more individuals test positive for PRV RNA; prevalence within stocks varied and ranged from 2% to 73%. The overall prevalence of PRV RNA-positive individuals across the study was 3.4% (77 of 2,252 fish tested). Findings of PRV RNA were most common in coho (Oncorhynchus kisutch Walbaum) and Chinook (O. tshawytscha Walbaum) salmon.
Citation Information
| Publication Year | 2018 |
|---|---|
| Title | Molecular testing of adult Pacific salmon and trout (Oncorhynchus spp.) for several RNA viruses demonstrates widespread distribution of piscine orthoreovirus in Alaska and Washington |
| DOI | 10.1111/jfd.12740 |
| Authors | Maureen K. Purcell, Rachel L. Thompson, Joy Evered, John Kerwin, Ted R. Meyers, Bruce Stewart, James Winton |
| Publication Type | Article |
| Publication Subtype | Journal Article |
| Series Title | Journal of Fish Diseases |
| Index ID | 70195200 |
| Record Source | USGS Publications Warehouse |
| USGS Organization | Western Fisheries Research Center |
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Final Dataset: Molecular testing of adult Pacific salmon and trout (Oncorhynchus spp.) for several RNA viruses demonstrates widespread distribution of piscine orthoreovirus (PRV) in Alaska and Washington
Maureen K Purcell, Ph.D.
Deputy Center Director
Rachel Powers (Former Employee)
Biological Science Technician
James Winton, Ph.D.
Research Microbiologist, Emeritus
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Final Dataset: Molecular testing of adult Pacific salmon and trout (Oncorhynchus spp.) for several RNA viruses demonstrates widespread distribution of piscine orthoreovirus (PRV) in Alaska and Washington
Dataset for the publication 'Molecular testing of adult Pacific salmon and trout (Oncorhynchus spp.) for several RNA viruses demonstrates widespread distribution of piscine orthoreovirus (PRV) in Alaska and Washington'. This research was initiated in conjunction with a systematic, multi-agency surveillance effort in the United States (U.S.) in response to reported findings of infectious salmon ane - Connect
Maureen K Purcell, Ph.D.
Deputy Center DirectorEmailRachel Powers (Former Employee)
Biological Science TechnicianJames Winton, Ph.D.
Research Microbiologist, EmeritusEmailPhone