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James Winton, Ph.D.

Jim works with scientists, technicians, post-doctoral researchers, graduate students and visiting scientists working to improve methods for the detection of fish pathogens, determine factors affecting the epidemiology of fish diseases, and develop novel control strategies for reducing losses among both hatchery-reared and wild fish.

Research Interests          

Infectious diseases of fish, fish cell and tissue culture, molecular taxonomy and epidemiology of fish pathogens, ecology of fish diseases in wild populations.

Mentorship/Outreach

  • 1995 to Present - Professor (Affiliate Faculty), University of Washington, School of Aquatic and Fishery Sciences, Seattle, WA
  • 1983-1986 - Assistant Professor, Oregon State University, Department of Microbiology, Corvallis, OR

Professional Experience

  • 2017 to Present - Senior Scientists, Emeritus

  • 1996-2017 - Chief, Fish Health Section, U.S. Geological Survey, Western Fisheries Research Center, Seattle, WA

  • 1995 to Present - Professor (Affiliate Faculty), University of Washington, School of Aquatic and Fishery Sciences, Seattle, WA

  • 1986-1996 - Project Leader, Infectious Diseases of Fish. U.S. Fish and Wildlife Service, National Fisheries Research Center, Seattle, WA

  • 1983-1986 - Assistant Professor, Oregon State University, Department of Microbiology, Corvallis, OR

  • 1984 to Present - Canadian Fish Health Official - International shipments of salmonid fish and gametes

  • 1981 to Present - U.S. Title 50 Inspector - International shipments of salmonid fish and gametes

  • 2010 - Pacific Salmon Commission - Invited expert to panel investigating Fraser River sockeye declines

  • 1991-2000 - Office International des Epizooties (OIE), Paris - Elected to Fish Diseases Commission

  • 1990-2003 - American Type Culture Collection (ATCC) - Animal Virus Advisory Group

  • 1988-2000 - International Committee on Taxonomy of Viruses (ICTV) - Reovirus Study Group

Education and Certifications

  • Ph.D. 1981. Oregon State University, Corvallis, OR

  • B.A. 1967. University of Colorado, Boulder, CO

Affiliations and Memberships*

  • American Association for the Advancement of Science

  • American Society for Microbiology

  • American Fisheries Society

  • European Association of Fish Pathologists

  • Japanese Society of Fish Pathologists

  • Wildlife Disease Association

Honors and Awards

  • 2015 - Senior Scientist – U.S. Geological Survey

  • 2006 - Department of Interior Distinguished Service Award

  • 2000 - American Fisheries Society Fish Health Section S. F. Snieszko Distinguished Service Award

  • 1999 - Department of Interior Meritorious Service Award

Science and Products

link
Cells used to isolate a fish virus die when infected with the picornavirus

Newly Discovered Picornavirus Spread by Baitfish

Scientists from the U.S. Geological Survey (USGS) contributed to a publication that reports the complete gene sequence of a novel picornavirus isolated from minnows and baitfish in several areas of the United States. The scientists used the molecular sequence and characterization of this virus to determine the evolutionary (phylogenetic) placement in the "family tree" of known fish viruses. The...
By
Ecosystems Mission Area, Contaminant Biology, Environmental Health Program, Toxic Substances Hydrology
link

Newly Discovered Picornavirus Spread by Baitfish

Scientists from the U.S. Geological Survey (USGS) contributed to a publication that reports the complete gene sequence of a novel picornavirus isolated from minnows and baitfish in several areas of the United States. The scientists used the molecular sequence and characterization of this virus to determine the evolutionary (phylogenetic) placement in the "family tree" of known fish viruses. The...
Learn More

Pufferfish mortality data

In 2010, a mass mortality of pufferfish in Hawaii was dominated by Arothron hispidus showing aberrant neurological behaviors. Using pathology, toxinology, and field surveys, we implicated a series of novel, polar, marine toxins as a likely cause of this mass mortality. Our findings are striking in that 1) a marine toxin was associated with a kill of a fish species that is, itself, toxic; 2) we
By
Ecosystems Mission Area, Biological Threats and Invasive Species Research Program, National Wildlife Health Center, Western Fisheries Research Center, Fish Health Program (FHP)
Cells used to isolate a fish virus die when infected with the picornavirus
Cells Used to Isolate a Fish Virus Die When Infected with Picornavirus
Cells Used to Isolate a Fish Virus Die When Infected with Picornavirus
Cells used to isolate a fish virus die when infected with the picornavirus

Cells Used to Isolate a Fish Virus Die When Infected with Picornavirus

Cells Used to Isolate a Fish Virus Die When Infected with Picornavirus

Cells used to isolate a fish virus die when infected with the picornavirus. 

By
Contaminant Biology, Toxic Substances Hydrology, Environmental Health Program
Cells used to isolate a fish virus die when infected with the picornavirus

Cells Used to Isolate a Fish Virus Die When Infected with Picornavirus

Cells Used to Isolate a Fish Virus Die When Infected with Picornavirus

Cells used to isolate a fish virus die when infected with the picornavirus. 

By
Contaminant Biology, Toxic Substances Hydrology, Environmental Health Program
Filter Total Items: 164

Susceptibility of Pallid Sturgeon to viral hemorrhagic septicemia virus genotype IVb

ObjectiveViral hemorrhagic septicemia virus (VHSV) is an aquatic rhabdovirus causing severe disease in freshwater and saltwater fish species. The susceptibility of endangered Pallid Sturgeon Scaphirhynchus albus to VHSV genotype IVb (VHSV-IVb) infection was investigated.MethodsAn in vitro assessment using two Pallid Sturgeon cell lines derived from skin and spleen tissue and in vivo evaluation of
Authors
Lacey R. Hopper, Jolene A. Glenn, Elizabeth MacConnell, James Winton, Eveline J. Emmenegger
By
Ecosystems Mission Area, Western Fisheries Research Center, Fish Health Program (FHP)

Effects of stocking density on stress response and susceptibility to infectious hematopoietic necrosis virus in rainbow trout

The goals of this study were to examine the effect of stocking density on the stress response and disease susceptibility in juvenile rainbow trout (Oncorhynchus mykiss). Fish were sorted into one of 2 stocking densities (high density "HD", 20-40 kg/m3) or (low density, "LD", 4-8 kg/m3) and 3 stress indices (cortisol levels in serum and water, and neutrophil: lymphocyte (N:L) ratios from blood smea
Authors
Jenna J Klug, Piper M Treuting, George E. Sanders, James Winton, Gael Kurath
By
Ecosystems Mission Area, Species Management Research Program, Western Fisheries Research Center, Fish Health Program (FHP)

Pacific herring Clupea pallasii are not susceptible to vibriosis from Vibrio anguillarum or V. ordalii under laboratory conditions

The ubiquity of Vibrio spp. throughout the coastal marine waters of the Pacific Northwest of North America raises questions about the susceptibility of native marine fishes, including Pacific herring (Clupea pallasii). Early reports of Vibriolike disease (Rucker et al., 1954; Walford, 1958) and Vibrio sp. isolations (Pacha & Kiehn, 1969) in Pacific herring remain questionable because both occurred
Authors
Paul Hershberger, M.E.T Stinson, Brenda L Hall, Ashley MacKenzie, Jacob L. Gregg, William August Richards, James Winton
By
Ecosystems Mission Area, Biological Threats and Invasive Species Research Program, Species Management Research Program, Western Fisheries Research Center, Fish Health Program (FHP), Marrowstone Marine Field Station (MMFS)

Consequences of Piscine orthoreovirus genotype 1 (PRV‐1) infections in Chinook salmon (Oncorhynchus tshawytscha ), coho salmon (O. kisutch ) and rainbow trout (O. mykiss )

Piscine orthoreovirus genotype 1 (PRV‐1) is the causative agent of heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon (Salmo salar L.). The virus has also been found in Pacific salmonids in western North America, raising concerns about the risk to native salmon and trout. Here, we report the results of laboratory challenges using juvenile Chinook salmon, coho salmon and rainbo
Authors
Maureen K. Purcell, Rachel L. Powers, Torunn Taksdal, Douglas Mckenney, Carla M. Conway, Diane G. Elliott, Mark Polinski, Kyle A. Garver, James Winton
By
Ecosystems Mission Area, Species Management Research Program, Western Fisheries Research Center, Fish Health Program (FHP)

Increased prespawning mortality threatens an integrated natural- and hatchery-origin sockeye salmon population in the Lake Washington Basin

The life cycle of diadromous fishes such as salmonids involves natural mortality in a series of distinct life history stages, occurring sequentially in different habitats. Decades of research have emphasized mortality at the embryo, juvenile, and sub-adult stages but it is increasingly clear that some adults that survive and return to freshwater habitats die during the final homeward migration or
Authors
Heidy K Barnett, Thomas P. Quinn, Mary Bhuthimethee, James Winton
By
Ecosystems Mission Area, Species Management Research Program, Western Fisheries Research Center, Fish Health Program (FHP)

Effect of growth rate on transcriptomic responses to immune stimulation in wild-type, domesticated, and GH-transgenic coho salmon

Background Transcriptomic responses to immune stimulation were investigated in coho salmon (Oncorhynchus kisutch) with distinct growth phenotypes. Wild-type fish were contrasted to strains with accelerated growth arising either from selective breeding (i.e. domestication) or genetic modification. Such distinct routes to accelerated growth may have unique implications for relationships and/or trade
Authors
Jin-Hyoung Kim, Daniel J Macqueen, James Winton, John D. Hansen, Hyun Park, Robert H Devlin
By
Ecosystems Mission Area, Species Management Research Program, Western Fisheries Research Center, Fish Health Program (FHP)

Complete genome sequences of the index isolates of two genotypes of Pacific salmon paramyxovirus

We report here the genome sequences of two index strains of Pacific salmon paramyxovirus isolated in 1982 and 1983 from adult salmon in Oregon. The isolates are most closely related to Atlantic salmon paramyxovirus, the type species of the genus Aquaparamyxovirus, but are sufficiently distinct to be considered two genotypes of a novel species.
Authors
James Winton, William N. Batts, Rachel L. Powers, Maureen K. Purcell
By
Ecosystems Mission Area, Biological Threats and Invasive Species Research Program, Species Management Research Program, Western Fisheries Research Center, Fish Health Program (FHP)

Development and characterization of two cell lines from gills of Atlantic salmon

Gill disease in Atlantic salmon, Salmo salar L., causes big losses in the salmon farming industry. Until now, tools to cultivate microorganisms causing gill disease and models to study the gill responses have been lacking. Here we describe the establishment and characterization of two cell lines from the gills of Atlantic salmon. Atlantic salmon gill cell ASG-10 consisted of cells staining for cyt
Authors
Mona C. Gjessing, Maria Aamelfot, William N. Batts, Sylvie L. Benestad, Ole B. Dale, Even Thoen, Simon C. Weli, James R. Winton
By
Ecosystems Mission Area, Species Management Research Program, Western Fisheries Research Center, Fish Health Program (FHP)

Molecular testing of adult Pacific salmon and trout (Oncorhynchus spp.) for several RNA viruses demonstrates widespread distribution of piscine orthoreovirus in Alaska and Washington

This research was initiated in conjunction with a systematic, multiagency surveillance effort in the United States (U.S.) in response to reported findings of infectious salmon anaemia virus (ISAV) RNA in British Columbia, Canada. In the systematic surveillance study reported in a companion paper, tissues from various salmonids taken from Washington and Alaska were surveyed for ISAV RNA using the U
Authors
Maureen K. Purcell, Rachel L. Thompson, Joy Evered, John Kerwin, Ted R. Meyers, Bruce Stewart, James Winton
By
Ecosystems Mission Area, Biological Threats and Invasive Species Research Program, Species Management Research Program, Western Fisheries Research Center, Fish Health Program (FHP)

A systematic surveillance programme for infectious salmon anaemia virus supports its absence in the Pacific Northwest of the United States

In response to reported findings of infectious salmon anaemia virus (ISAV) in British Columbia (BC), Canada, in 2011, U.S. national, state and tribal fisheries managers and fish health specialists developed and implemented a collaborative ISAV surveillance plan for the Pacific Northwest region of the United States. Accordingly, over a 3-1/2-year period, 4,962 salmonids were sampled and successfull
Authors
Lori L. Gustafson, Lynn H. Creekmore, Kevin R. Snekvik, Jayde A. Ferguson, Janet V. Warg, Marilyn Blair, Theodore R. Meyers, Bruce Stewart, Kenneth I. Warheit, John Kerwin, Andrew E. Goodwin, Linda D. Rhodes, Janet E. Whaley, Maureen K. Purcell, Collette Bentz, Desiree Shasa, Joel Bader, James R. Winton
By
Ecosystems Mission Area, Species Management Research Program, Western Fisheries Research Center, Fish Health Program (FHP)

Isolation and characterization of the fall Chinook aquareovirus

BackgroundSalmon are paramount to the economy, ecology, and history of the Pacific Northwest. Viruses constitute one of the major threats to salmon health and well-being, with more than twenty known virus species that infect salmon. Here, we describe the isolation and characterization of the fall Chinook aquareovirus, a divergent member of the species Aquareovirus B within the family Reoviridae.Me
Authors
Negar Makhsous, Nicole L. Jensen, Katherine H. Haman, William N. Batts, Keith R. Jerome, James Winton, Alexander L. Greninger
By
Ecosystems Mission Area, Biological Threats and Invasive Species Research Program, Species Management Research Program, Western Fisheries Research Center, Fish Health Program (FHP)

Analytical validation of a reverse transcriptase droplet digital PCR (RT-ddPCR) for quantitative detection of infectious hematopoietic necrosis virus

Infectious hematopoietic necrosis virus (IHNV) is an important pathogen of salmonid fishes. A validated universal reverse transcriptase quantitative PCR (RT-qPCR) assay that can quantify levels of IHNV in fish tissues has been previously reported. In the present study, we adapted the published set of IHNV primers and probe for use in a reverse-transcriptase droplet digital PCR (RT-ddPCR) assay for
Authors
Peng Jia, Maureen K. Purcell, Guang Pan, Jinjin Wang, Shifu Kan, Yin Liu, Xiaocong Zheng, Xiujie SHi, Junqiang He, Li Yu, Qunyi Hua, Tikang Lu, Wensheng Lan, James Winton, Ningyi Jin, Hong Liu
By
Ecosystems Mission Area, Species Management Research Program, Western Fisheries Research Center, Fish Health Program (FHP)
Western Fisheries Science News, February 2017 | Issue 5.2

Western Fisheries Science News, February 2017 | Issue 5.2

In Memoriam — William Toshio Yasutake, 1922–2016

Read Article
Western Fisheries Science News, September 2014 | Issue 2.9

Western Fisheries Science News, September 2014 | Issue 2.9

WFRC Hosts Visiting Scientists from Norway

Read Article
Western Fisheries Science News, July 2014 | Issue 2.7

Western Fisheries Science News, July 2014 | Issue 2.7

WFRC – People’s Republic of China IHN Reference Laboratory Twinning Project

Read Article

Science and Products

link
Cells used to isolate a fish virus die when infected with the picornavirus

Newly Discovered Picornavirus Spread by Baitfish

Scientists from the U.S. Geological Survey (USGS) contributed to a publication that reports the complete gene sequence of a novel picornavirus isolated from minnows and baitfish in several areas of the United States. The scientists used the molecular sequence and characterization of this virus to determine the evolutionary (phylogenetic) placement in the "family tree" of known fish viruses. The...
By
Ecosystems Mission Area, Contaminant Biology, Environmental Health Program, Toxic Substances Hydrology
link

Newly Discovered Picornavirus Spread by Baitfish

Scientists from the U.S. Geological Survey (USGS) contributed to a publication that reports the complete gene sequence of a novel picornavirus isolated from minnows and baitfish in several areas of the United States. The scientists used the molecular sequence and characterization of this virus to determine the evolutionary (phylogenetic) placement in the "family tree" of known fish viruses. The...
Learn More

Pufferfish mortality data

In 2010, a mass mortality of pufferfish in Hawaii was dominated by Arothron hispidus showing aberrant neurological behaviors. Using pathology, toxinology, and field surveys, we implicated a series of novel, polar, marine toxins as a likely cause of this mass mortality. Our findings are striking in that 1) a marine toxin was associated with a kill of a fish species that is, itself, toxic; 2) we
By
Ecosystems Mission Area, Biological Threats and Invasive Species Research Program, National Wildlife Health Center, Western Fisheries Research Center, Fish Health Program (FHP)
Cells used to isolate a fish virus die when infected with the picornavirus
Cells Used to Isolate a Fish Virus Die When Infected with Picornavirus
Cells Used to Isolate a Fish Virus Die When Infected with Picornavirus
Cells used to isolate a fish virus die when infected with the picornavirus

Cells Used to Isolate a Fish Virus Die When Infected with Picornavirus

Cells Used to Isolate a Fish Virus Die When Infected with Picornavirus

Cells used to isolate a fish virus die when infected with the picornavirus. 

By
Contaminant Biology, Toxic Substances Hydrology, Environmental Health Program
Cells used to isolate a fish virus die when infected with the picornavirus

Cells Used to Isolate a Fish Virus Die When Infected with Picornavirus

Cells Used to Isolate a Fish Virus Die When Infected with Picornavirus

Cells used to isolate a fish virus die when infected with the picornavirus. 

By
Contaminant Biology, Toxic Substances Hydrology, Environmental Health Program
Filter Total Items: 164

Susceptibility of Pallid Sturgeon to viral hemorrhagic septicemia virus genotype IVb

ObjectiveViral hemorrhagic septicemia virus (VHSV) is an aquatic rhabdovirus causing severe disease in freshwater and saltwater fish species. The susceptibility of endangered Pallid Sturgeon Scaphirhynchus albus to VHSV genotype IVb (VHSV-IVb) infection was investigated.MethodsAn in vitro assessment using two Pallid Sturgeon cell lines derived from skin and spleen tissue and in vivo evaluation of
Authors
Lacey R. Hopper, Jolene A. Glenn, Elizabeth MacConnell, James Winton, Eveline J. Emmenegger
By
Ecosystems Mission Area, Western Fisheries Research Center, Fish Health Program (FHP)

Effects of stocking density on stress response and susceptibility to infectious hematopoietic necrosis virus in rainbow trout

The goals of this study were to examine the effect of stocking density on the stress response and disease susceptibility in juvenile rainbow trout (Oncorhynchus mykiss). Fish were sorted into one of 2 stocking densities (high density "HD", 20-40 kg/m3) or (low density, "LD", 4-8 kg/m3) and 3 stress indices (cortisol levels in serum and water, and neutrophil: lymphocyte (N:L) ratios from blood smea
Authors
Jenna J Klug, Piper M Treuting, George E. Sanders, James Winton, Gael Kurath
By
Ecosystems Mission Area, Species Management Research Program, Western Fisheries Research Center, Fish Health Program (FHP)

Pacific herring Clupea pallasii are not susceptible to vibriosis from Vibrio anguillarum or V. ordalii under laboratory conditions

The ubiquity of Vibrio spp. throughout the coastal marine waters of the Pacific Northwest of North America raises questions about the susceptibility of native marine fishes, including Pacific herring (Clupea pallasii). Early reports of Vibriolike disease (Rucker et al., 1954; Walford, 1958) and Vibrio sp. isolations (Pacha & Kiehn, 1969) in Pacific herring remain questionable because both occurred
Authors
Paul Hershberger, M.E.T Stinson, Brenda L Hall, Ashley MacKenzie, Jacob L. Gregg, William August Richards, James Winton
By
Ecosystems Mission Area, Biological Threats and Invasive Species Research Program, Species Management Research Program, Western Fisheries Research Center, Fish Health Program (FHP), Marrowstone Marine Field Station (MMFS)

Consequences of Piscine orthoreovirus genotype 1 (PRV‐1) infections in Chinook salmon (Oncorhynchus tshawytscha ), coho salmon (O. kisutch ) and rainbow trout (O. mykiss )

Piscine orthoreovirus genotype 1 (PRV‐1) is the causative agent of heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon (Salmo salar L.). The virus has also been found in Pacific salmonids in western North America, raising concerns about the risk to native salmon and trout. Here, we report the results of laboratory challenges using juvenile Chinook salmon, coho salmon and rainbo
Authors
Maureen K. Purcell, Rachel L. Powers, Torunn Taksdal, Douglas Mckenney, Carla M. Conway, Diane G. Elliott, Mark Polinski, Kyle A. Garver, James Winton
By
Ecosystems Mission Area, Species Management Research Program, Western Fisheries Research Center, Fish Health Program (FHP)

Increased prespawning mortality threatens an integrated natural- and hatchery-origin sockeye salmon population in the Lake Washington Basin

The life cycle of diadromous fishes such as salmonids involves natural mortality in a series of distinct life history stages, occurring sequentially in different habitats. Decades of research have emphasized mortality at the embryo, juvenile, and sub-adult stages but it is increasingly clear that some adults that survive and return to freshwater habitats die during the final homeward migration or
Authors
Heidy K Barnett, Thomas P. Quinn, Mary Bhuthimethee, James Winton
By
Ecosystems Mission Area, Species Management Research Program, Western Fisheries Research Center, Fish Health Program (FHP)

Effect of growth rate on transcriptomic responses to immune stimulation in wild-type, domesticated, and GH-transgenic coho salmon

Background Transcriptomic responses to immune stimulation were investigated in coho salmon (Oncorhynchus kisutch) with distinct growth phenotypes. Wild-type fish were contrasted to strains with accelerated growth arising either from selective breeding (i.e. domestication) or genetic modification. Such distinct routes to accelerated growth may have unique implications for relationships and/or trade
Authors
Jin-Hyoung Kim, Daniel J Macqueen, James Winton, John D. Hansen, Hyun Park, Robert H Devlin
By
Ecosystems Mission Area, Species Management Research Program, Western Fisheries Research Center, Fish Health Program (FHP)

Complete genome sequences of the index isolates of two genotypes of Pacific salmon paramyxovirus

We report here the genome sequences of two index strains of Pacific salmon paramyxovirus isolated in 1982 and 1983 from adult salmon in Oregon. The isolates are most closely related to Atlantic salmon paramyxovirus, the type species of the genus Aquaparamyxovirus, but are sufficiently distinct to be considered two genotypes of a novel species.
Authors
James Winton, William N. Batts, Rachel L. Powers, Maureen K. Purcell
By
Ecosystems Mission Area, Biological Threats and Invasive Species Research Program, Species Management Research Program, Western Fisheries Research Center, Fish Health Program (FHP)

Development and characterization of two cell lines from gills of Atlantic salmon

Gill disease in Atlantic salmon, Salmo salar L., causes big losses in the salmon farming industry. Until now, tools to cultivate microorganisms causing gill disease and models to study the gill responses have been lacking. Here we describe the establishment and characterization of two cell lines from the gills of Atlantic salmon. Atlantic salmon gill cell ASG-10 consisted of cells staining for cyt
Authors
Mona C. Gjessing, Maria Aamelfot, William N. Batts, Sylvie L. Benestad, Ole B. Dale, Even Thoen, Simon C. Weli, James R. Winton
By
Ecosystems Mission Area, Species Management Research Program, Western Fisheries Research Center, Fish Health Program (FHP)

Molecular testing of adult Pacific salmon and trout (Oncorhynchus spp.) for several RNA viruses demonstrates widespread distribution of piscine orthoreovirus in Alaska and Washington

This research was initiated in conjunction with a systematic, multiagency surveillance effort in the United States (U.S.) in response to reported findings of infectious salmon anaemia virus (ISAV) RNA in British Columbia, Canada. In the systematic surveillance study reported in a companion paper, tissues from various salmonids taken from Washington and Alaska were surveyed for ISAV RNA using the U
Authors
Maureen K. Purcell, Rachel L. Thompson, Joy Evered, John Kerwin, Ted R. Meyers, Bruce Stewart, James Winton
By
Ecosystems Mission Area, Biological Threats and Invasive Species Research Program, Species Management Research Program, Western Fisheries Research Center, Fish Health Program (FHP)

A systematic surveillance programme for infectious salmon anaemia virus supports its absence in the Pacific Northwest of the United States

In response to reported findings of infectious salmon anaemia virus (ISAV) in British Columbia (BC), Canada, in 2011, U.S. national, state and tribal fisheries managers and fish health specialists developed and implemented a collaborative ISAV surveillance plan for the Pacific Northwest region of the United States. Accordingly, over a 3-1/2-year period, 4,962 salmonids were sampled and successfull
Authors
Lori L. Gustafson, Lynn H. Creekmore, Kevin R. Snekvik, Jayde A. Ferguson, Janet V. Warg, Marilyn Blair, Theodore R. Meyers, Bruce Stewart, Kenneth I. Warheit, John Kerwin, Andrew E. Goodwin, Linda D. Rhodes, Janet E. Whaley, Maureen K. Purcell, Collette Bentz, Desiree Shasa, Joel Bader, James R. Winton
By
Ecosystems Mission Area, Species Management Research Program, Western Fisheries Research Center, Fish Health Program (FHP)

Isolation and characterization of the fall Chinook aquareovirus

BackgroundSalmon are paramount to the economy, ecology, and history of the Pacific Northwest. Viruses constitute one of the major threats to salmon health and well-being, with more than twenty known virus species that infect salmon. Here, we describe the isolation and characterization of the fall Chinook aquareovirus, a divergent member of the species Aquareovirus B within the family Reoviridae.Me
Authors
Negar Makhsous, Nicole L. Jensen, Katherine H. Haman, William N. Batts, Keith R. Jerome, James Winton, Alexander L. Greninger
By
Ecosystems Mission Area, Biological Threats and Invasive Species Research Program, Species Management Research Program, Western Fisheries Research Center, Fish Health Program (FHP)

Analytical validation of a reverse transcriptase droplet digital PCR (RT-ddPCR) for quantitative detection of infectious hematopoietic necrosis virus

Infectious hematopoietic necrosis virus (IHNV) is an important pathogen of salmonid fishes. A validated universal reverse transcriptase quantitative PCR (RT-qPCR) assay that can quantify levels of IHNV in fish tissues has been previously reported. In the present study, we adapted the published set of IHNV primers and probe for use in a reverse-transcriptase droplet digital PCR (RT-ddPCR) assay for
Authors
Peng Jia, Maureen K. Purcell, Guang Pan, Jinjin Wang, Shifu Kan, Yin Liu, Xiaocong Zheng, Xiujie SHi, Junqiang He, Li Yu, Qunyi Hua, Tikang Lu, Wensheng Lan, James Winton, Ningyi Jin, Hong Liu
By
Ecosystems Mission Area, Species Management Research Program, Western Fisheries Research Center, Fish Health Program (FHP)
Western Fisheries Science News, February 2017 | Issue 5.2

Western Fisheries Science News, February 2017 | Issue 5.2

In Memoriam — William Toshio Yasutake, 1922–2016

Read Article
Western Fisheries Science News, September 2014 | Issue 2.9

Western Fisheries Science News, September 2014 | Issue 2.9

WFRC Hosts Visiting Scientists from Norway

Read Article
Western Fisheries Science News, July 2014 | Issue 2.7

Western Fisheries Science News, July 2014 | Issue 2.7

WFRC – People’s Republic of China IHN Reference Laboratory Twinning Project

Read Article

*Disclaimer: Listing outside positions with professional scientific organizations on this Staff Profile are for informational purposes only and do not constitute an endorsement of those professional scientific organizations or their activities by the USGS, Department of the Interior, or U.S. Government

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