We have described in previous reports (Schill et al., 1998) the development of a polymerase chain reaction (PCR) amplification of 18S ribosomal RNA for the detection of Myxozoan parasites. Oligonucleotide primers were developed by multiple alignment of Myxozoan sequence information and analysis by a custom-written computer program (PRIM). Candidate pairs of primer sequences were then analyzed for specificity by BLAST (Basic Local Alignment Search Tool). From these, a set of promising primers (MYXFWD and MYXREV) was chosen for further testing. These were chosen because they should direct detection of a number of Myxozoan species (Table 1). PCR using MXYFWD and MYXREV proved to be robust and relatively free of artifact products. Further, we were able to routinely detect Myxobolus cerebralis in fish tissues (Figure 1).
|Title||Non-lethal sampling for the detection of <i>Myxobolus cerebralis</i> in asymptomatic rainbow trout|
|Authors||Bane Schill, Thomas Waldrop, Christine Densmore, Vicki Blazer|
|Publication Subtype||Conference publication|
|Record Source||USGS Publications Warehouse|
|USGS Organization||Leetown Science Center|