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Occurrence and distribution of fecal indicator bacteria and gene markers of pathogenic bacteria in Great Lakes tributaries, March-October 2011

February 20, 2015

From March through October 2011, the U.S. Geological Survey (USGS), conducted a study to determine the frequency of occurrence of pathogen gene markers and densities of fecal indicator bacteria (FIB) in 22 tributaries to the Great Lakes. This project was funded as part of the Great Lakes Restoration Initiative (GLRI) and included sampling at 22 locations throughout 6 states that border the Great Lakes.

A total of 177 environmental samples were collected at USGS streamgaging stations during both normal-flow and high-flow conditions and were analyzed by the Michigan Bacteriological Research Laboratory at the USGS Water Science Center in Lansing, Michigan.

Water samples were analyzed for the presence of FIB concentrations (FIB; fecal coliform bacteria, Escherichia coli [E. coli], and enterococci) by using membrane filtration and serial dilution methods. The resulting enrichments from standard culturing of the samples were then analyzed by using polymerase chain reaction (PCR) to determine the occurrence of pathogen gene markers for Shigella species, Campylobacter jejuni and coli, Salmonellaspecies, and pathogenic E. coli, including Shiga toxin-producing E. coli (STEC).

Citation Information

Publication Year 2015
Title Occurrence and distribution of fecal indicator bacteria and gene markers of pathogenic bacteria in Great Lakes tributaries, March-October 2011
DOI 10.3133/ofr20151013
Authors Angela K. Brennan, Heather E. Johnson, Alexander R. Totten, Joseph W. Duris
Publication Type Report
Publication Subtype USGS Numbered Series
Series Title Open-File Report
Series Number 2015-1013
Index ID ofr20151013
Record Source USGS Publications Warehouse
USGS Organization Michigan Water Science Center