On July 23, 1947, a 1‐mile section of Back Creek, treated the previous year with a DDT suspension with little damage to fish, was re‐treated with an oil spray at the same rate (1 pound per acre). The oil spray killed about six times as many fish as the suspension formula.
Fish began dying within 3 to 4 hours after treatment, whereas several days elapsed before mortality began with the suspension formula in 1946. In addition to larger numbers of the minnow species, some adult game fish were killed. Losses of fish were not as great below the sprayed section as when a suspension formula was used, probably because a good part of the DDT was drifted ashore by breezes or by water currents.
Some species of fish such as bluntnose minnows (Hyborhynchus notatus), chub suckers (Erimyzon oblongus), and pickerel (Esox niger), although numerous, were relatively unaffected. Considerable mortality of largemouth bass (Micropterus salmoides) and bluegill sunfish (Lepomis macrochirus) occurred in liveboxes placed in the sprayed section, particularly at the lower stations. Largemouth bass nearly 4 inches long survived at a station where 54.0 percent of the 2‐inch bass were killed. This fact supplied some positive evidence that fish of larger size are better able to withstand DDT.