TanA: a fluorogenic probe for thiaminase activity
January 1, 2013
A fluorogenic thiamine analogue is presented as a fluorescent probe for thiaminase activity. The emission of the fluorophore is quenched by photoinduced electron transfer (PET) to the N-substituted pyridinium portion of the probe. Action of the enzyme releases the free pyridine group causing a substantial increase in fluorescence.
Citation Information
| Publication Year | 2013 |
|---|---|
| Title | TanA: a fluorogenic probe for thiaminase activity |
| DOI | 10.1039/C2AY26047F |
| Authors | Wanjun Zhu, James L. Zajicek, Donald E. Tillitt, Timothy E. Glass |
| Publication Type | Article |
| Publication Subtype | Journal Article |
| Series Title | Analytical Methods |
| Index ID | 70048566 |
| Record Source | USGS Publications Warehouse |
| USGS Organization | Columbia Environmental Research Center |