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Untargeted lipidomics for determining cellular and sub-cellular responses in Zebrafish (Danio rerio) liver cells following exposure to complex mixtures in U.S. streams

June 7, 2021

Surface waters often contain a variety of chemical contaminants potentially capable of producing adverse outcomes in both humans and wildlife due to impacts from industrial, urban, and agricultural activity. Here, we report the results of a zebrafish liver (ZFL) cell-based lipidomics approach to assess the potential ecotoxicological effects of complex contaminant mixtures using water collected from eight impacted streams across the United States mainland and Puerto Rico. We initially characterized the ZFL lipidome using high resolution mass spectrometry, resulting in the annotation of 508 lipid species covering 27 classes. We then identified lipid changes induced by all streamwater samples (nonspecific stress indicators) as well as those unique to water samples taken from specific streams. Subcellular impacts were classified based on organelle-specific lipid changes, including increased lipid saturation (endoplasmic reticulum stress), elevated bis(monoacylglycero)phosphate (lysosomal overload), decreased ubiquinone (mitochondrial dysfunction), and elevated ether lipids (peroxisomal stress). Finally, we demonstrate how these results can uniquely inform environmental monitoring and risk assessments of surface waters.

Citation Information

Publication Year 2021
Title Untargeted lipidomics for determining cellular and sub-cellular responses in Zebrafish (Danio rerio) liver cells following exposure to complex mixtures in U.S. streams
DOI 10.1021/acs.est.1c01132
Authors Huajun Zhen, Quincy Teng, Jonathan D Mosley, Timothy W. Collette, Yang Yue, Paul Bradley, Drew R. Ekman
Publication Type Article
Publication Subtype Journal Article
Series Title Environmental Science & Technology
Series Number
Index ID 70221221
Record Source USGS Publications Warehouse
USGS Organization South Atlantic Water Science Center

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