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Use of real-time PCR to detect canine parvovirus in feces of free-ranging wolves

May 31, 2012

Using real-time PCR, we tested 15 wolf (Canis lupus) feces from the Superior National Forest (SNF), Minnesota, USA, and 191 from Yellowstone National Park (YNP), USA, collected during summer and 13 during winter for canine parvovirus (CPV)-2 DNA. We also tested 20 dog feces for CPV-2 DNA. The PCR assay was 100% sensitive and specific with a minimum detection threshold of 104 50% tissue culture infective dose. Virus was detected in two winter specimens but none of the summer specimens. We suggest applying the technique more broadly especially with winter feces.

Citation Information

Publication Year 2012
Title Use of real-time PCR to detect canine parvovirus in feces of free-ranging wolves
DOI 10.7589/0090-3558-48.2.473
Authors L. David Mech, Emily S. Almberg, Douglas Smith, Sagar Goyal, Randall S. Singer
Publication Type Article
Publication Subtype Journal Article
Series Title Journal of Wildlife Diseases
Series Number
Index ID 70009688
Record Source USGS Publications Warehouse
USGS Organization Northern Prairie Wildlife Research Center