Skip to main content
U.S. flag

An official website of the United States government

Utilization of integrated correlative light and electron microscopy (iCLEM) for imaging sedimentary organic matter

July 30, 2017

We report here a new microscopic technique for imaging and identifying sedimentary organic matter in geologic materials that combines inverted fluorescence microscopy with scanning electron microscopy and allows for sequential imaging of the same region of interest without transferring the sample between instruments. This integrated correlative light and electron microscopy technique is demonstrated with observations from an immature lacustrine oil shale from the Eocene Green River Mahogany Zone and mid‐oil window paralic shale from the Upper Cretaceous Tuscaloosa Group. This technique has the potential to allow for identification and characterization of organic matter in shale hydrocarbon reservoirs that is not possible using either light or electron microscopy alone, and may be applied to understanding the organic matter type and thermal regime in which organic nanoporosity forms, thereby reducing uncertainty in the estimation of undiscovered hydrocarbon resources.

Publication Year 2017
Title Utilization of integrated correlative light and electron microscopy (iCLEM) for imaging sedimentary organic matter
DOI 10.1111/jmi.12576
Authors Paul C. Hackley, Brett J. Valentine, Leonard M. Voortman, Daan van Oosten Slingeland, Javin J. Hatcherian
Publication Type Article
Publication Subtype Journal Article
Series Title Journal of Microscopy
Index ID 70219142
Record Source USGS Publications Warehouse
USGS Organization Geology, Energy & Minerals Science Center