Amanda J Boone
I am Ecologist in the eDNA laboratory at the USGS Forest and Rangeland Ecosystem Science Center.
Science and Products
Data supporting "Detecting bumble bees in the wild using environmental DNA: development and validation of a qPCR assay for the endangered Franklin's bumble bee (Bombus franklini)" Data supporting "Detecting bumble bees in the wild using environmental DNA: development and validation of a qPCR assay for the endangered Franklin's bumble bee (Bombus franklini)"
This dataset includes quantitative polymerase chain reaction (qPCR) data for an in vitro test of the Bombus franklini eDNA assay using target and nontarget DNA extract, qPCR data for a plate used to calculate the limits of detection and quantification of the assay based on serial 10-fold dilutions of B. franklini gBlock, qPCR data for an in situ test of the assay using flower samples...
Comparison of environmental DNA sampling protocols for detection of stream fishes in the Pacific Northwest, 2019 (ver. 2.0, November 2025) Comparison of environmental DNA sampling protocols for detection of stream fishes in the Pacific Northwest, 2019 (ver. 2.0, November 2025)
This dataset contains environmental DNA (eDNA) data (field, extraction, and qPCR) for rainbow trout sampling efforts conducted in the Pacific Northwest in 2019. Samples were collected using three eDNA sampling methods. Also included are qPCR data for extraction negatives, no-template controls (NTC), and standard dilutions of rainbow trout gBlock. NTC and gBlock qPCR data were used to...
eDNA and visual encounter survey detection of amphibians and chytrid fungus in Colorado canyonlands, 2019-2022 (ver. 1.1, November 2025) eDNA and visual encounter survey detection of amphibians and chytrid fungus in Colorado canyonlands, 2019-2022 (ver. 1.1, November 2025)
This dataset contains environmental DNA (eDNA) and visual encounter survey (VES) data collected between 2019 and 2022 in Colorado canyonlands. eDNA data (field, extraction, and qPCR) is for three target species: American bullfrog, northern leopard frog, and Great Basin spadefoot. VES data is for six target species: American bullfrog, northern leopard frog, Great Basin spadefoot, red...
Detecting bumble bees in the wild using environmental DNA: Development and validation of a qPCR assay for the endangered Franklin’s bumble bee (Bombus franklini) Detecting bumble bees in the wild using environmental DNA: Development and validation of a qPCR assay for the endangered Franklin’s bumble bee (Bombus franklini)
Environmental DNA (eDNA) sampling is a noninvasive alternative to conventional methods of surveying insects that may be particularly useful for detecting pollinators. We developed a quantitative polymerase chain reaction (qPCR) assay to detect the DNA of Franklin’s bumble bee (Bombus franklini) from flower samples and conducted an initial test of the assay using samples collected within...
Authors
Michaela Ray Grossklaus, David S. Pilliod, Stephen Frank Spear, Matthew B B. Laramie, Akhil Kholwadwala, Amanda Jean Boone, Yer Lor, Marissa Kaminski, Jeffrey G. Everett
Science and Products
Data supporting "Detecting bumble bees in the wild using environmental DNA: development and validation of a qPCR assay for the endangered Franklin's bumble bee (Bombus franklini)" Data supporting "Detecting bumble bees in the wild using environmental DNA: development and validation of a qPCR assay for the endangered Franklin's bumble bee (Bombus franklini)"
This dataset includes quantitative polymerase chain reaction (qPCR) data for an in vitro test of the Bombus franklini eDNA assay using target and nontarget DNA extract, qPCR data for a plate used to calculate the limits of detection and quantification of the assay based on serial 10-fold dilutions of B. franklini gBlock, qPCR data for an in situ test of the assay using flower samples...
Comparison of environmental DNA sampling protocols for detection of stream fishes in the Pacific Northwest, 2019 (ver. 2.0, November 2025) Comparison of environmental DNA sampling protocols for detection of stream fishes in the Pacific Northwest, 2019 (ver. 2.0, November 2025)
This dataset contains environmental DNA (eDNA) data (field, extraction, and qPCR) for rainbow trout sampling efforts conducted in the Pacific Northwest in 2019. Samples were collected using three eDNA sampling methods. Also included are qPCR data for extraction negatives, no-template controls (NTC), and standard dilutions of rainbow trout gBlock. NTC and gBlock qPCR data were used to...
eDNA and visual encounter survey detection of amphibians and chytrid fungus in Colorado canyonlands, 2019-2022 (ver. 1.1, November 2025) eDNA and visual encounter survey detection of amphibians and chytrid fungus in Colorado canyonlands, 2019-2022 (ver. 1.1, November 2025)
This dataset contains environmental DNA (eDNA) and visual encounter survey (VES) data collected between 2019 and 2022 in Colorado canyonlands. eDNA data (field, extraction, and qPCR) is for three target species: American bullfrog, northern leopard frog, and Great Basin spadefoot. VES data is for six target species: American bullfrog, northern leopard frog, Great Basin spadefoot, red...
Detecting bumble bees in the wild using environmental DNA: Development and validation of a qPCR assay for the endangered Franklin’s bumble bee (Bombus franklini) Detecting bumble bees in the wild using environmental DNA: Development and validation of a qPCR assay for the endangered Franklin’s bumble bee (Bombus franklini)
Environmental DNA (eDNA) sampling is a noninvasive alternative to conventional methods of surveying insects that may be particularly useful for detecting pollinators. We developed a quantitative polymerase chain reaction (qPCR) assay to detect the DNA of Franklin’s bumble bee (Bombus franklini) from flower samples and conducted an initial test of the assay using samples collected within...
Authors
Michaela Ray Grossklaus, David S. Pilliod, Stephen Frank Spear, Matthew B B. Laramie, Akhil Kholwadwala, Amanda Jean Boone, Yer Lor, Marissa Kaminski, Jeffrey G. Everett