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Pacific Northwest Environmental DNA Laboratory

We are a premier environmental DNA (eDNA) laboratory working with federal and non-federal partners to meet goals for early detection of invasive species, natural resources management, conservation, and restoration in the western U.S.  

News

New Effort - Using Artificial Flowers to Survey for Pollinators at Solar Installations

New Effort - Using Artificial Flowers to Survey for Pollinators at Solar Installations

Bipartisan Infrastructure Law investments combine science and technology to track biological threats in US waters

Bipartisan Infrastructure Law investments combine science and technology to track biological threats in US waters

Detecting Invasive and Rare Species with the National Streamflow Network

Detecting Invasive and Rare Species with the National Streamflow Network

Publications

Accounting for temporal variation and correlation in environmental DNA sampling can improve ecological inferences Accounting for temporal variation and correlation in environmental DNA sampling can improve ecological inferences

Environmental DNA (eDNA) concentration varies through space and time, and measurements collected close together are often correlated. Ignoring this dependence can inflate the rate of incorrect ecological inferences (Type I error rate). Although spatial correlation in eDNA has received considerable attention, temporal correlation has been less well studied. Statistical models and study...
Authors
Ben C. Augustine, Patrick R. Hutchins, Margaret E. Hunter, Christopher M. Merkes, David S. Pilliod, Stephen Frank Spear, Caitlin E. Beaver, Scott D. George, Devin Nicole Jones-Slobodian, Adam J. Sepulveda

Detecting bumble bees in the wild using environmental DNA: Development and validation of a qPCR assay for the endangered Franklin’s bumble bee (Bombus franklini) Detecting bumble bees in the wild using environmental DNA: Development and validation of a qPCR assay for the endangered Franklin’s bumble bee (Bombus franklini)

Environmental DNA (eDNA) sampling is a noninvasive alternative to conventional methods of surveying insects that may be particularly useful for detecting pollinators. We developed a quantitative polymerase chain reaction (qPCR) assay to detect the DNA of Franklin’s bumble bee (Bombus franklini) from flower samples and conducted an initial test of the assay using samples collected within...
Authors
Michaela Ray Grossklaus, David S. Pilliod, Stephen Frank Spear, Matthew B B. Laramie, Akhil Kholwadwala, Amanda Jean Boone, Yer Lor, Marissa Kaminski, Jeffrey G. Everett

Amphibian diversity of the western Colorado canyonlands including potential threats from nonnative bullfrogs and disease Amphibian diversity of the western Colorado canyonlands including potential threats from nonnative bullfrogs and disease

Throughout the canyons of the Colorado and Uncompahgre Plateaus, water is a limited resource for wildlife, with patchy distribution and seasonal availability. Tributary creeks within these canyons drain into mainstem rivers, providing habitat and breeding sites for native amphibians. Yet, little is known about the diversity and distribution of amphibians that live in these harsh, dynamic
Authors
Denita M Weeks, David S. Pilliod, Madeline (Nikki) Grant-Hoffman, Anjelica F Quintana Spencer, Daniel Neubaum, Paul Hampton, Michaela Ray Grossklaus, Matthew B B. Laramie, Erin L. Muths
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