Brenda Berlowski-Zier
Brenda Berlowski-Zier is a Biological Science Laboratory Technician at the National Wildlife Health Center.
Science and Products
Avian-associated Aspergillus fumigatus displays broad phylogenetic distribution, no evidence for host specificity, and multiple genotypes within epizootic events
Birds are highly susceptible to aspergillosis, which can manifest as a primary infection in both domestic and wild birds. Aspergillosis in wild birds causes mortalities ranging in scale from single animals to large-scale epizootic events. However, pathogenicity factors associated with aspergillosis in wild birds have not been examined. Specifically, it is unknown whether wild bird-infecting strain
Authors
Lotus A. Lofgren, Jeffrey M. Lorch, Robert A. Cramer, David S. Blehert, Brenda M. Berlowski-Zier, Megan Winzeler, Cecilia Gutierrez-Perez, Nicole E. Kordana, Jason E. Stajich
Salmonella enterica serovar Typhimurium from wild birds in the United States represent distinct lineages defined by bird type
Salmonella enterica serovar Typhimurium is typically considered a host generalist; however, certain isolates are associated with specific hosts and show genetic features of host adaptation. Here, we sequenced 131 S. Typhimurium isolates from wild birds collected in 30 U.S. states during 1978-2019. We found that isolates from broad taxonomic host groups including passerine birds, water birds (Aequo
Authors
Yezhi Fu, Nkuchia M. M’ikanatha, Jeffrey M. Lorch, David S. Blehert, Brenda M. Berlowski-Zier, Chris A. Whitehouse, Shaoting Li, Xiangyu Deng, Jared C. Smith, Nikki W. Shariat, Erin M. Nawrocki, Edward G. Dudley
Low occurrence of multi-antimicrobial and heavy metal resistance in Salmonella enterica from wild birds in the United States
Wild birds are common reservoirs of Salmonella enterica. Wild birds carrying resistant S. enterica may pose a risk to public health as they can spread the resistant bacteria across large spatial scales within a short time. Here, we whole-genome sequenced 375 S. enterica strains from wild birds collected in 41 U.S. states during 1978–2019 to examine bacterial resistance to antibiotics and heavy met
Authors
Yezhi Fu, Nkuchia M M’ikanatha, Chris A Whitehouse, Heather Tate, Andrea Ottensen, Jeffrey M. Lorch, David S. Blehert, Brenda M. Berlowski-Zier, Edward G. Dudley
Analysis of archival specimens confirms White-nose syndrome in little brown bats (Myotis lucifugus) from New York, USA, in spring 2007
White-nose syndrome (WNS), an emerging fungal disease of North American bats, was first diagnosed in January 2008, although mortality and photo-documentation suggest the disease may have been present earlier. Using archived samples, we describe a definitive case of WNS in little brown bats (Myotis lucifugus) from New York, USA, in spring 2007.
Authors
Saskia Keller, Jeffrey M. Lorch, Brenda M. Berlowski-Zier, Anne Ballmann, David S. Blehert
A novel host-adapted strain of Salmonella Typhimurium causes disease in olive ridley turtles (Lepidochelys olivacea) in the Pacific.
Salmonella spp. are frequently shed by wildlife including turtles, but S. enterica subsp. enterica serovar Typhimurium or lesions associated with Salmonella are rare in turtles. Between 1996 and 2016, we necropsied 127 apparently healthy pelagic olive ridley turtles (Lepidochelys olivacea) that died from drowning bycatch in fisheries and 44 live or freshly dead stranded turtles from the west coast
Authors
Thierry M. Work, Julie Dagenais, Brian A. Stacy, Jason T. Ladner, Jeffrey M. Lorch, George H. Balazs, Elias Barquero-Calvo, Brenda M. Berlowski-Zier, Renee Breeden, Natalia Corrales-Gómez, Rocio Gonzalez-Barrientos, Heather Harris, Gabriela Hernández-Mora, Angel Herrera-Ulloa, Shoreh Hesami, T. Todd Jones, Juan Alberto Morales, Terry M. Norton, Robert Rameyer, Daniel Taylor, Thomas B. Waltzek
Detection of Bisgaard taxon 40 in Rhinoceros Auklets (Cerorhinca monocerata) with pneumonia and septicemia from a mortality event in Washington, USA
We isolated Bisgaard taxon 40 from Rhinoceros Auklets (Cerorhinca monocerata) with pneumonia and septicemia from Washington, US, found dead in 2016. Previously isolated only from the respiratory tract of a gull (Laridae), little is known about its pathogenic potential and whether it acts as a primary or opportunistic pathogen.
Authors
Susan Knowles, Barbara Bodenstein, Brenda M. Berlowski-Zier, Susan M Thomas, Scott F Pearson, Jeffrey M. Lorch
Avian cholera causes marine bird mortality in the Bering Sea of Alaska
The first known avian cholera outbreak among wild birds in Alaska occurred during November 2013. Liver, intestinal, and splenic necrosis consistent with avian cholera was noted, and Pasteurella multocida serotype 1 was isolated from liver and lung or spleen in Crested Auklets (Aethia cristatella), Thick-billed Murres (Uria lomvia), Common Eider (Somateria mollissima), Northern Fulmars (Fulmarus gl
Authors
Barbara L. Bodenstein, Kimberlee B. Beckmen, Gay Sheffield, Kathy Kuletz, Caroline R. Van Hemert, Brenda M. Berlowski-Zier, Valerie I. Shearn-Bochsler
Acute pasteurellosis in wild big brown bats (Eptesicus fuscus)
We report acute fatal pasteurellosis in wild big brown bats (Eptesicus fuscus) in Wisconsin, USA. Mortality of approximately 100 bats was documented over 4 wk, with no evidence for predatory injuries. Pasteurella multocida serotype 1 was isolated from multiple internal organs from four of five bats examined postmortem.
Authors
David S. Blehert, Ramón P. Maluping, David E. Green, Brenda M. Berlowski-Zier, Anne E. Ballmann, Julia Langenberg
In vitro detection and quantification of botulinum neurotoxin type E activity in avian blood
Botulinum neurotoxin serotype E (BoNT/E) outbreaks in the Great Lakes region cause large annual avian mortality events, with an estimated 17,000 bird deaths reported in 2007 alone. During an outbreak investigation, blood collected from bird carcasses is tested for the presence of BoNT/E using the mouse lethality assay. While sensitive, this method is labor-intensive and low throughput and can take
Authors
Timothy M. Piazza, David S. Blehert, F. Mark Dunning, Brenda M. Berlowski-Zier, Fusun N. Zeytin, Michael D. Samuel, Ward C. Tucker
In vitro detection and quantification of botulinum neurotoxin type E activity in avian blood
Botulinum neurotoxin serotype E (BoNT/E) outbreaks in the Great Lakes region cause large annual avian mortality events, with an estimated 17,000 bird deaths reported in 2007 alone. During an outbreak investigation, blood collected from bird carcasses is tested for the presence of BoNT/E using the mouse lethality assay. While sensitive, this method is labor-intensive and low throughput and can take
Authors
Timothy M. Piazza, David S. Blehert, F. Mark Dunning, Brenda M. Berlowski-Zier, Fusun N. Zeytin, M. D. Samuel, Ward C. Tucker
Using amplified fragment length polymorphism analysis to differentiate isolates of Pasteurella multocida serotype 1
Avian cholera, an infectious disease caused by the bacterium Pasteurella multocida, kills thousands of North American wild waterfowl annually. Pasteurella multocida serotype 1 isolates cultured during a laboratory challenge study of Mallards (Anas platyrhynchos) and collected from wild birds and environmental samples during avian cholera outbreaks were characterized using amplified fragment length
Authors
David S. Blehert, K. L. Jefferson, Dennis M. Heisey, M. D. Samuel, Brenda M. Berlowski-Zier, Daniel J. Shadduck
Science and Products
- Publications
Avian-associated Aspergillus fumigatus displays broad phylogenetic distribution, no evidence for host specificity, and multiple genotypes within epizootic events
Birds are highly susceptible to aspergillosis, which can manifest as a primary infection in both domestic and wild birds. Aspergillosis in wild birds causes mortalities ranging in scale from single animals to large-scale epizootic events. However, pathogenicity factors associated with aspergillosis in wild birds have not been examined. Specifically, it is unknown whether wild bird-infecting strainAuthorsLotus A. Lofgren, Jeffrey M. Lorch, Robert A. Cramer, David S. Blehert, Brenda M. Berlowski-Zier, Megan Winzeler, Cecilia Gutierrez-Perez, Nicole E. Kordana, Jason E. StajichSalmonella enterica serovar Typhimurium from wild birds in the United States represent distinct lineages defined by bird type
Salmonella enterica serovar Typhimurium is typically considered a host generalist; however, certain isolates are associated with specific hosts and show genetic features of host adaptation. Here, we sequenced 131 S. Typhimurium isolates from wild birds collected in 30 U.S. states during 1978-2019. We found that isolates from broad taxonomic host groups including passerine birds, water birds (AequoAuthorsYezhi Fu, Nkuchia M. M’ikanatha, Jeffrey M. Lorch, David S. Blehert, Brenda M. Berlowski-Zier, Chris A. Whitehouse, Shaoting Li, Xiangyu Deng, Jared C. Smith, Nikki W. Shariat, Erin M. Nawrocki, Edward G. DudleyLow occurrence of multi-antimicrobial and heavy metal resistance in Salmonella enterica from wild birds in the United States
Wild birds are common reservoirs of Salmonella enterica. Wild birds carrying resistant S. enterica may pose a risk to public health as they can spread the resistant bacteria across large spatial scales within a short time. Here, we whole-genome sequenced 375 S. enterica strains from wild birds collected in 41 U.S. states during 1978–2019 to examine bacterial resistance to antibiotics and heavy metAuthorsYezhi Fu, Nkuchia M M’ikanatha, Chris A Whitehouse, Heather Tate, Andrea Ottensen, Jeffrey M. Lorch, David S. Blehert, Brenda M. Berlowski-Zier, Edward G. DudleyAnalysis of archival specimens confirms White-nose syndrome in little brown bats (Myotis lucifugus) from New York, USA, in spring 2007
White-nose syndrome (WNS), an emerging fungal disease of North American bats, was first diagnosed in January 2008, although mortality and photo-documentation suggest the disease may have been present earlier. Using archived samples, we describe a definitive case of WNS in little brown bats (Myotis lucifugus) from New York, USA, in spring 2007.AuthorsSaskia Keller, Jeffrey M. Lorch, Brenda M. Berlowski-Zier, Anne Ballmann, David S. BlehertA novel host-adapted strain of Salmonella Typhimurium causes disease in olive ridley turtles (Lepidochelys olivacea) in the Pacific.
Salmonella spp. are frequently shed by wildlife including turtles, but S. enterica subsp. enterica serovar Typhimurium or lesions associated with Salmonella are rare in turtles. Between 1996 and 2016, we necropsied 127 apparently healthy pelagic olive ridley turtles (Lepidochelys olivacea) that died from drowning bycatch in fisheries and 44 live or freshly dead stranded turtles from the west coastAuthorsThierry M. Work, Julie Dagenais, Brian A. Stacy, Jason T. Ladner, Jeffrey M. Lorch, George H. Balazs, Elias Barquero-Calvo, Brenda M. Berlowski-Zier, Renee Breeden, Natalia Corrales-Gómez, Rocio Gonzalez-Barrientos, Heather Harris, Gabriela Hernández-Mora, Angel Herrera-Ulloa, Shoreh Hesami, T. Todd Jones, Juan Alberto Morales, Terry M. Norton, Robert Rameyer, Daniel Taylor, Thomas B. WaltzekDetection of Bisgaard taxon 40 in Rhinoceros Auklets (Cerorhinca monocerata) with pneumonia and septicemia from a mortality event in Washington, USA
We isolated Bisgaard taxon 40 from Rhinoceros Auklets (Cerorhinca monocerata) with pneumonia and septicemia from Washington, US, found dead in 2016. Previously isolated only from the respiratory tract of a gull (Laridae), little is known about its pathogenic potential and whether it acts as a primary or opportunistic pathogen.AuthorsSusan Knowles, Barbara Bodenstein, Brenda M. Berlowski-Zier, Susan M Thomas, Scott F Pearson, Jeffrey M. LorchAvian cholera causes marine bird mortality in the Bering Sea of Alaska
The first known avian cholera outbreak among wild birds in Alaska occurred during November 2013. Liver, intestinal, and splenic necrosis consistent with avian cholera was noted, and Pasteurella multocida serotype 1 was isolated from liver and lung or spleen in Crested Auklets (Aethia cristatella), Thick-billed Murres (Uria lomvia), Common Eider (Somateria mollissima), Northern Fulmars (Fulmarus glAuthorsBarbara L. Bodenstein, Kimberlee B. Beckmen, Gay Sheffield, Kathy Kuletz, Caroline R. Van Hemert, Brenda M. Berlowski-Zier, Valerie I. Shearn-BochslerAcute pasteurellosis in wild big brown bats (Eptesicus fuscus)
We report acute fatal pasteurellosis in wild big brown bats (Eptesicus fuscus) in Wisconsin, USA. Mortality of approximately 100 bats was documented over 4 wk, with no evidence for predatory injuries. Pasteurella multocida serotype 1 was isolated from multiple internal organs from four of five bats examined postmortem.AuthorsDavid S. Blehert, Ramón P. Maluping, David E. Green, Brenda M. Berlowski-Zier, Anne E. Ballmann, Julia LangenbergIn vitro detection and quantification of botulinum neurotoxin type E activity in avian blood
Botulinum neurotoxin serotype E (BoNT/E) outbreaks in the Great Lakes region cause large annual avian mortality events, with an estimated 17,000 bird deaths reported in 2007 alone. During an outbreak investigation, blood collected from bird carcasses is tested for the presence of BoNT/E using the mouse lethality assay. While sensitive, this method is labor-intensive and low throughput and can takeAuthorsTimothy M. Piazza, David S. Blehert, F. Mark Dunning, Brenda M. Berlowski-Zier, Fusun N. Zeytin, Michael D. Samuel, Ward C. TuckerIn vitro detection and quantification of botulinum neurotoxin type E activity in avian blood
Botulinum neurotoxin serotype E (BoNT/E) outbreaks in the Great Lakes region cause large annual avian mortality events, with an estimated 17,000 bird deaths reported in 2007 alone. During an outbreak investigation, blood collected from bird carcasses is tested for the presence of BoNT/E using the mouse lethality assay. While sensitive, this method is labor-intensive and low throughput and can takeAuthorsTimothy M. Piazza, David S. Blehert, F. Mark Dunning, Brenda M. Berlowski-Zier, Fusun N. Zeytin, M. D. Samuel, Ward C. TuckerUsing amplified fragment length polymorphism analysis to differentiate isolates of Pasteurella multocida serotype 1
Avian cholera, an infectious disease caused by the bacterium Pasteurella multocida, kills thousands of North American wild waterfowl annually. Pasteurella multocida serotype 1 isolates cultured during a laboratory challenge study of Mallards (Anas platyrhynchos) and collected from wild birds and environmental samples during avian cholera outbreaks were characterized using amplified fragment lengthAuthorsDavid S. Blehert, K. L. Jefferson, Dennis M. Heisey, M. D. Samuel, Brenda M. Berlowski-Zier, Daniel J. Shadduck