This dataset contains 253 samples from 11 occurrences of Chloropyron molle subsp. molle, and 3 occurrences of Chloropyron molle hispidum. Genomic markers were generated from ddRAD loci and analyzed using the Stacks v2.60 pipeline. The genotypes for all samples are provided in a VCF file with 40,707 independent loci . A companion sample data file is provided with sample names, and occurrence designations, and other data. These files may be opened and edited in a text editor program, such as Notepad (PC) or BBEdit (Mac). The .vcf file can be loaded into the Stacks population program to calculate genetic diversity statistics, or loaded into R, using vcfR, for further analysis.