Data on the detection of Epioblasma capsaeformis using a designed environmental DNA (eDNA) survey upstream and downstream of known populations in the Clinch River, Virginia and Tennessee, 2020 to 2022 (ver 2.0, February 2026)

These data describe a designed environmental DNA (eDNA) survey for the detection of oyster mussel (Epioblasma capsaeformis) from field collected water samples. Parameters described include the limit of detection and limit of quantification of the assay; a list of freshwater mussel species tissue samples that were used to test specificity of the assay; and field collected water samples that were tested to detect the presence of oyster mussel DNA. Samples were collected from sites on the Clinch River, Tennessee from 2020 to 2022.