Taylor, J.A., Richter, C.A., Suzuki, A., Watanabe, H., Iguchi, T., Coser, K.R., Shioda, T., and vom Saal, F.S., 2012, Dose-related estrogen effects on gene expression in fetal mouse prostate mesenchymal cells: PLOS ONE, v. 7, no. 10, p. e48311
Cathy Richter, Ph.D.
Science and Products
Date published: May 25, 2021
Threatened, Endangered or Rare Species Detection
The non-invasive nature of eDNA methods for species detection is a great advantage for monitoring of rare, threatened, or endangered species.
Contacts: Cathy Richter, Ph.D., Katy Klymus
Attribution: Columbia Environmental Research Center
Date published: April 29, 2021
Searching for Shiners in Missouri
Researchers from the University of Central Missouri (UCM), in collaboration with the Missouri Department of Conservation (MDC) and Columbia Environmental Research Center (CERC), implement environmental DNA (eDNA) methods to identify and manage Topeka Shiner populations in Missouri.
Contacts: Cathy Richter, Ph.D., Jacob Westhoff, PhD, Aaron Geheber, PhD, Austin Mueller
Attribution: Columbia Environmental Research Center
Date published: December 16, 2020
Status: Active Molecular Section: eDNA Research
Environmental DNA (eDNA) research at CERC focuses on the development and utility of eDNA tools as well as interpretation of eDNA data in real-world management applications. We work with academic, state, federal and international partners in developing standards and best practices for eDNA technology and exploring the factors that affect eDNA detection in the field. Our lab's eDNA research...
Contacts: Cathy Richter, Ph.D., Katy Klymus
Attribution: Columbia Environmental Research Center
Date published: August 23, 2018
Status: Active Fishing and Hunting Science Team
The Fishing and Hunting integrated science team focuses on contaminant and pathogen exposures in the environment that could impact the presence and vitality of fish and wildlife populations that drive commercial, recreational and subsistence activities such as hunting and fishing. If actual risks are identified this project will inform how to economically and effectively minimize...
Contacts: Vicki S Blazer, Cathy Richter, Ph.D.
Date published: December 17, 2020
Metabarcoding data from freshwater mussel eDNA collected in Clinch River, Virginia 2017
Data from metabarcoding assays to detect a suite of mussel species using mitochondrial DNA regions of the cytochrome c oxidase subunit I (COI) and NADH dehydrogenase subunit (ND1) genes sequences.
Attribution: Columbia Environmental Research Center
Date published: November 19, 2020
Mucket eDNA detection in Wallen’s Bend, Clinch River, Tennessee, September 2019
The data describe the technical performance of a designed qPCR assay for the detection of mucket (Actinonaias ligamentina) eDNA from field collected water samples. Parameters described include the sequences of the primers and probes used; the limit of detection and limit of quantification for the assay; a list of freshwater mussels species that were used to test specificity of the assay%3
Attribution: Contaminant Biology, Columbia Environmental Research Center
Date published: September 24, 2020
Effects of early life stage exposure of largemouth bass to atrazine or a model estrogen (17α-ethinylestradiol)
Largemouth base histological development and transcriptomic changes in gonad tissue after early life stage exposure to Atrazine (1-Chloro-3-ethylamino-5-isopropylamino-2,4,6-triazine) or the model estrogen 17α-ethinylestradiol.
Date published: August 28, 2020
Asian carp eDNA and egg morphology data collected from Truman Reservoir tributaries, Missouri, USA, 2014
Ichthyoplankton tows samples collected from Truman reservoir tributaries evaluated for eDNA. Data also includes hydrologic and water quality parameters and morphology of grass carp eggs.
Attribution: Columbia Environmental Research Center
Year Published: 2021
Grass carp reproduction in small tributaries of Truman Reservoir, Missouri: Implications for establishment in novel habitats
Substantial work has been conducted to estimate the river length required for recruitment of invasive Grass Carp and bigheaded carp; however, the distance upstream and size of stream required for spawning remain unclear. Adult Grass Carp are regularly captured in Truman Reservoir, Missouri although there have been only unconfirmed sightings of...
Hayer, Cari-Ann; Bayless, Michael F.; Richter, Catherine A.; George, Amy E.; Chapman, Duane C.
Year Published: 2021
Reproduction
Lake charr Salvelinus namaycush are typically fall spawners although one ecotype has populations that spawn during spring and fall (siscowets in Lake Superior). Lake charr are iteroparous (reproduce more than once in a lifetime) with group-synchronous ovarian development and typically spawn once per year. However, lake charr may not...
W. Goetz, Frederick; Marsden, J. Ellen; Richter, Catherine A.; Tillitt, Donald E.; Sitar, Shawn P.; Riley, Stephen; Krueger, Charles C.
Year Published: 2021
Metabarcoding assays for the detection of freshwater mussels (Unionida) with environmental DNA
Freshwater mussels of the order Unionida are a widely distributed taxon that are important in maintaining freshwater ecosystems and are also highly imperiled throughout the world. Monitoring of mussel populations with environmental DNA (eDNA) is an attractive alternative to traditional methods because it is noninvasive and requires less labor and...
Klymus, Katy E.; Richter, Catherine A.; Thompson, Nathan; Hinck, Jo Ellen; Jones, Jess W.
Year Published: 2020
Development and testing of species-specific quantitative PCR assays for environmental DNA applications
New, non-invasive methods for detecting and monitoring species presence are being developed to aid in fisheries and wildlife conservation management. The use of environmental DNA (eDNA) samples for detecting macrobiota is one such group of methods that is rapidly becoming popular and being implemented in national management programs. Here we focus...
Klymus, Katy E.; Ruiz Ramos, Dannise Vannesa; Thompson, Nathan; Richter, Catherine A.
Year Published: 2020
Effects of early life stage exposure of largemouth bass to atrazine or a model estrogen (17α-ethinylestradiol)
Endocrine disrupting contaminants are of continuing concern for potentially contributing to reproductive dysfunction in largemouth and smallmouth bass in the Chesapeake Bay watershed (CBW) and elsewhere. Exposures to atrazine (ATR) have been hypothesized to have estrogenic effects on vertebrate endocrine systems. The incidence of intersex in male...
Leet, Jessica Kristin; Richter, Catherine A.; Cornman, Robert S.; Berninger, Jason P.; Bhandari, Ramji K.; Nicks, Diane K.; Zajicek, James L.; Blazer, Vicki S.; Tillitt, Donald E.
Year Published: 2020
Use of environmental DNA to detect grass carp spawning events
The timing and location of spawning events are important data for managers seeking to control invasive grass carp populations. Ichthyoplankton tows for grass carp eggs and larvae can be used to detect spawning events; however, these samples can be highly debris-laden, and are expensive and laborious to process. An alternative method, environmental...
Hayer, Cari-Ann; Bayless, Michael F.; George, Amy E.; Thompson, Nathan; Richter, Catherine A.; Chapman, Duane C.
Year Published: 2019
Reporting the limits of detection and quantification for environmental DNA assays
BackgroundEnvironmental DNA (eDNA) analysis is increasingly being used to detect the presence and relative abundance of rare species, especially invasive or imperiled aquatic species. The rapid progress in the eDNA field has resulted in numerous studies impacting conservation and management actions. However, standardization of eDNA methods and...
Klymus, Katy E.; Merkes, Christopher M.; Allison, Michael J.; Goldberg, Caren S.; Helbing, Caren C.; Hunter, Margaret; Jackson, Craig; Lance, Richard F.; Mangan, Anna M.; Monroe, Emy M.; Piaggio, Antoinette J.; Stokdyk, Joel P.; Wilson, Chris C.; Richter, Catherine A.
Year Published: 2018
Thiamine deficiency in fishes: Causes, consequences, and potential solutions
Thiamine deficiency complex (TDC) is a disorder resulting from the inability to acquire or retain thiamine (vitamin B1) and has been documented in organisms in aquatic ecosystems ranging from the Baltic Sea to the Laurentian Great Lakes. The biological mechanisms leading to TDC emergence may vary among systems, but in fishes, one common outcome is...
Christie, Mark R.; Harder, Avril M.; Ardren, William R.; Evans, Allison N.; Futia, Matthew H.; Kraft, Clifford E.; Marsden, J. Ellen; Richter, Catherine A.; Rinchard, Jacques; Tillitt, Donald E.; Christie, Mark R.View Citation
Harder, A.M., Ardren, W.R., Evans, A.N., Futia, M.H., Kraft, C.E., Marsden, J.E., Richter, C.A., Rinchard, J., Tillitt, D.E., and Christie, M.R., 2018, Thiamine deficiency in fishes: causes, consequences, and potential solutions: Reviews in Fish Biology and Fisheries
Year Published: 2017
Metabarcoding of environmental DNA samples to explore the use of uranium mine containment ponds as a water source for wildlife
Understanding how anthropogenic impacts on the landscape affect wildlife requires a knowledge of community assemblages. Species surveys are the first step in assessing community structure, and recent molecular applications such as metabarcoding and environmental DNA analyses have been proposed as an additional and complementary wildlife survey...
Klymus, Katy E.; Richter, Catherine A.; Thompson, Nathan; Hinck, Jo EllenAttribution: Columbia Environmental Research Center, Ecosystems, Contaminant Biology, Region 4: Mississippi Basin
View Citation
Klymus, K., Richter, C., Thompson, N., and Hinck, J., 2017, Metabarcoding of Environmental DNA Samples to Explore the Use of Uranium Mine Containment Ponds as a Water Source for Wildlife: Diversity, v. 9, no. 4, 54
Year Published: 2017
Experimental observations on the decay of environmental DNA from bighead and silver carps
Interest in the field of environmental DNA (eDNA) is growing rapidly and eDNA surveys are becoming an important consideration for aquatic resource managers dealing with invasive species. However, in order for eDNA monitoring to mature as a research and management tool, there are several critical knowledge gaps that must be filled. One such gap is...
Lance, Richard F.; Klymus, Katy E.; Richter, Catherine A.; Guan, Xin; Farrington, Heather L.; Carr, Matthew R.; Thompson, Nathan; Chapman, Duane C.; Baerwaldt, Kelly L.View Citation
Lance, R.F., Klymus, K.E., Richter, C.A., Guan, X., Farrington, H.L., Carr, M.R., Thompson, N., Chapman, D.C., and Baerwaldt, K.L., 2017, Experimental observations on the decay of environmental DNA from bighead and silver carps, Management of Biological Invasions 8(3): 343-359
Year Published: 2016
Evaluation of potential mechanisms of atrazine-induced reproductive impairment in fathead minnow (Pimephales promelas) and Japanese medaka (Oryzias latipes)
Atrazine has been implicated in reproductive dysfunction of exposed organisms, and previous studies documented decreased egg production in Japanese medaka (Oryzias latipes) and fathead minnows (Pimephales promelas) during 30-d to 38-d exposures to 0.5 µg/L, 5 µg/L, and 50 µg/L atrazine. The authors evaluated possible mechanisms underlying the...
Richter, Catherine A.; Papoulias, Diana M.; Whyte, Jeffrey J.; Tillitt, Donald E.Attribution: Columbia Environmental Research Center, Ecosystems, Contaminant Biology, Environmental Health Program, Region 4: Mississippi Basin
View Citation
Richter, C.A., Papoulias, D.M., Whyte, J.J., and Tillitt, D.E., 2016, Evaluation of Potential Mechanisms of Atrazine-Induced Reproductive Impairment in Fathead Minnow (Pimephales promelas) And Japanese Medaka (Oryzias latipes): Environmental Toxicology and Chemistry, Vol. 35, No. 9, pp. 2230-2238
Year Published: 2016
First direct confirmation of grass carp spawning in a Great Lakes tributary
Grass carp (Ctenopharyngodon idella), an invasive species of Asian carp, has been stocked for many decades in the United States for vegetation control. Adult individuals have been found in all of the Great Lakes except Lake Superior, but no self-sustaining populations have yet been identified in Great Lakes tributaries. In 2012, a commercial...
Embke, Holly S.; Kocovsky, Patrick M.; Richter, Catherine A.; Pritt, Jeremy J.; Christine M. Mayer; Qian, SongPre-USGS Publications
Taylor, J.A., Richter, C.A., Ruhlen, R.L., and vom Saal, F.S., 2011, Estrogenic environmental chemicals and drugs: Mechanisms for effects on the developing male urogenital system: The Journal of Steroid Biochemistry and Molecular Biology, v. 127, no. 1, p. 83-95
Richter, C.A., Taylor, J.A., Ruhlen, R.L., Welshons, W.V., and vom Saal, F.S., 2007, Estradiol and Bisphenol A stimulate androgen receptor and estrogen receptor gene expression in fetal mouse prostate mesenchyme cells: Environmental Health Perspectives, v. 115, no. 6, p. 902-908
vom Saal, F.S., Richter, C.A., Mao, J., and Welshons, W.V., 2005, Commercial animal feed: Variability in estrogenic activity and effects on body weight in mice: Birth Defects Research Part A: Clinical and Molecular Teratology, v. 73, no. 7, p. 474-475
Timms, B.G., Howdeshell, K.L., Barton, L., Bradley, S., Richter, C.A., and vom Saal, F.S., 2005, Estrogenic chemicals in plastic and oral contraceptives disrupt development of the fetal mouse prostate and urethra: Proceedings of the National Academy of Sciences of the United States of America, v. 102, no. 19, p. 7014-7019
Richter, C., Timms, B., and vom Saal, F., 2005, Prostate development: mechanisms for opposite effects of low and high doses of estrogenic chemicals, in Naz, R., ed., Endocrine Disruptors: Effects on Male and Female Reproductive Systems: New York, CRC Press, p. 379-410.
vom Saal, F.S., Richter, C.A., Ruhlen, R.R., Nagel, S.C., Timms, B.G., and Welshons, W.V., 2005, The importance of appropriate controls, animal feed, and animal models in interpreting results from low-dose studies of bisphenol A: Birth Defects Research Part A: Clinical and Molecular Teratology, v. 73, no. 3, p. 140-145
vom Saal, F.S., Richter, C.A., Ruhlen, R.R., Nagel, S.C., and Welshons, W.V., 2004, Disruption of laboratory experiments due to leaching of Bisphenol A from polycarbonate cages and bottles and uncontrolled variability in components of animal feed, in National Research Council, ed., The Development of Science-based Guidelines for Laboratory Animal Care: Proceedings of the November 2003 International Workshop: The National Academies Press, p. 65-69.