Cathy Richter, PhD

Science and Products

• Science
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  May 25, 2021

  Threatened, Endangered or Rare Species Detection

  The non-invasive nature of eDNA methods for species detection is a great advantage for monitoring of rare, threatened, or endangered species.

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  Columbia Environmental Research Center

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  May 25, 2021

  Threatened, Endangered or Rare Species Detection

  The non-invasive nature of eDNA methods for species detection is a great advantage for monitoring of rare, threatened, or endangered species.

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  April 29, 2021

  Searching for Shiners in Missouri

  Researchers from the University of Central Missouri (UCM), in collaboration with the Missouri Department of Conservation (MDC) and Columbia Environmental Research Center (CERC), implement environmental DNA (eDNA) methods to identify and manage Topeka Shiner populations in Missouri.

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  Columbia Environmental Research Center

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  April 29, 2021

  Searching for Shiners in Missouri

  Researchers from the University of Central Missouri (UCM), in collaboration with the Missouri Department of Conservation (MDC) and Columbia Environmental Research Center (CERC), implement environmental DNA (eDNA) methods to identify and manage Topeka Shiner populations in Missouri.

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  December 16, 2020

  Molecular Section: eDNA Research

  Environmental DNA (eDNA) research at CERC focuses on the development and utility of eDNA tools as well as interpretation of eDNA data in real-world management applications. We work with academic, state, federal and international partners in developing standards and best practices for eDNA technology and exploring the factors that affect eDNA detection in the field. Our lab's eDNA research covers...

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  Columbia Environmental Research Center

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  December 16, 2020

  Molecular Section: eDNA Research

  Environmental DNA (eDNA) research at CERC focuses on the development and utility of eDNA tools as well as interpretation of eDNA data in real-world management applications. We work with academic, state, federal and international partners in developing standards and best practices for eDNA technology and exploring the factors that affect eDNA detection in the field. Our lab's eDNA research covers...

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  August 23, 2018

  Fishing and Hunting Integrated Science Team

  The Fishing and Hunting integrated science team focuses on contaminant and pathogen exposures in the environment that could impact the presence and vitality of fish and wildlife populations that drive commercial, recreational and subsistence activities such as hunting and fishing. If actual risks are identified this project will inform how to economically and effectively minimize risk by providing...

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  Ecosystems Mission Area, Contaminant Biology, Environmental Health Program, Toxic Substances Hydrology

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  August 23, 2018

  Fishing and Hunting Integrated Science Team

  The Fishing and Hunting integrated science team focuses on contaminant and pathogen exposures in the environment that could impact the presence and vitality of fish and wildlife populations that drive commercial, recreational and subsistence activities such as hunting and fishing. If actual risks are identified this project will inform how to economically and effectively minimize risk by providing...

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• Data
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  January 30, 2023

  Thiaminase activity measurements in whole zebrafish

  Data describe results of a set of laboratory experiments using a previously described quantitative thiaminase I activity assay to test the thiaminase activity of whole zebrafish homogenates. Parameters described include the thiaminase activity data, dilution factors, incubation time, and tissue weight assayed. Experiments were conducted at the USGS Columbia Environmental Research Center, Columbia,

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  Columbia Environmental Research Center

  December 15, 2022

  Water chemistry and fish metrics data for adult largemouth bass exposed in outdoor ponds to 17alpha-ethinylestradiol or an estrone-atrazine mixture

  Physiological endpoints observed in adult largemouth bass in response to an EE2 (17 alpha-ethinylestradiol) or mixture of estrone and atrazine exposure under laboratory pond conditions. Also included are water quality and chemical concentration data. Adult LMB were exposed in outdoor pond mesocosms from post-spawning to early gonad recrudescence to either EE2 (2.4 nanograms per liter), or a mixtur

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  Columbia Environmental Research Center

  October 20, 2022

  Physiological and molecular endpoints observed in juvenile largemouth bass in response to an estrogen (17α-ethinylestradiol) and subsequently a bacterial challenge (Edwardsiella piscicida) exposure under laboratory conditions.

  Physiological and molecular endpoints observed in juvenile largemouth bass in response to an estrogen (17α-ethinylestradiol) and subsequently a bacterial challenge (Edwardsiella piscicida) exposure under laboratory conditions. Also included are water quality and chemical concentration data.

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  Columbia Environmental Research Center

  August 26, 2022

  Polyacrylamide gel electrophoresis results with thiaminase activity stain of recombinant putative thiaminases expressed in E. coli

  Data describe results of a set of laboratory experiments using polyacrylamide gel electrophoresis with a thiaminase activity stain to test the solubility and thiaminase activity of recombinant putative thiaminases expressed in E. coli. Parameters described include the presence and relative mobility of thiaminases, and the presence and relative mobility of recombinant proteins. Experiments were con

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  Columbia Environmental Research Center

  March 23, 2022

  Longnose darter (Percina nasuta) eDNA survey results from the St. Francis River, Missouri 2018

  Data describe a designed environmental DNA (eDNA) survey for the detection of longnose darter (Percina nasuta) in field collected water samples. Parameters described include the limit of detection and limit of quantification of the assay; a list of freshwater fish species tissue samples that were used to test specificity of the assay; and field collected water samples that were tested to detect th

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  Columbia Environmental Research Center

  December 17, 2020

  Metabarcoding data from freshwater mussel eDNA collected in Clinch River, Virginia 2017

  Data from metabarcoding assays to detect a suite of mussel species using mitochondrial DNA regions of the cytochrome c oxidase subunit I (COI) and NADH dehydrogenase subunit (ND1) genes sequences.

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  Ecosystems Mission Area, Contaminant Biology, Environmental Health Program, Toxic Substances Hydrology, Columbia Environmental Research Center

  November 20, 2020

  Mucket eDNA detection in Wallen's Bend, Clinch river, Tennessee, September 2019

  The data describe the technical performance of a designed qPCR assay for the detection of mucket (Actinonaias ligamentina) eDNA from field collected water samples. Parameters described include the sequences of the primers and probes used; the limit of detection and limit of quantification for the assay; a list of freshwater mussels species that were used to test specificity of the assay; and in si

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  Ecosystems Mission Area, Contaminant Biology, Environmental Health Program, Toxic Substances Hydrology, Columbia Environmental Research Center

  September 25, 2020

  Effects of early life stage exposure of largemouth bass to atrazine or a model estrogen (17a-ethinylestradiol)

  Largemouth base histological development and transcriptomic changes in gonad tissue after early life stage exposure to Atrazine (1-Chloro-3-ethylamino-5-isopropylamino-2,4,6-triazine) or the model estrogen 17a-ethinylestradiol.

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  Ecosystems Mission Area, Contaminant Biology, Columbia Environmental Research Center

  August 31, 2020

  Asian carp eDNA and egg morphology data collected from Truman Reservoir tributaries, Missouri, USA, 2014

  Ichthyoplankton tows samples collected from Truman reservoir tributaries evaluated for eDNA. Data also includes hydrologic and water quality parameters and morphology of grass carp eggs.

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  Columbia Environmental Research Center

  March 29, 2018

  eDNA sampling sites in the Grand Canyon region near breccia pipe uranium mines_2015_2016

  Geospatial locations of eDNA sampling sites in the Grand Canyon region near breccia pipe uranium mines

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  Columbia Environmental Research Center
• Publications
  Filter Total Items: 37

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  January 13, 2023

  Genetic basis of thiaminase I activity in a vertebrate, zebrafish Danio rerio

  Thiamine (vitamin B1) metabolism is an important driver of human and animal health and ecological functioning. Some organisms, including species of ferns, mollusks, and fish, contain thiamine-degrading enzymes known as thiaminases, and consumption of these organisms can lead to thiamine deficiency in the consumer. Consumption of fish containing thiaminase has led to elevated mortality and recruitm

  Authors

  Catherine A. Richter, Allison N. Evans, Scott A. Heppell, James L. Zajicek, Donald E. Tillitt

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  Ecosystems Mission Area, Columbia Environmental Research Center

  December 17, 2022

  Declines in reproductive condition of male largemouth bass (Micropterus salmoides) following seasonal exposure to estrogenic endocrine-disrupting compounds

  Reproductive abnormalities, that could lead to possible effects at the population level, have been observed in wild fish throughout the United States, with high prevalence in largemouth bass (LMB; Micropterus salmoides) and smallmouth bass (Micropterus dolomieu). Estrone (E1) and atrazine (ATR) are common environmental contaminants often associated with agricultural land use. 17alpha-ethinylestrad

  Authors

  Jessica Kristin Leet, Catherine A. Richter, Robert Gale, Donald E. Tillitt, Jill Jenkins

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  Ecosystems Mission Area, Columbia Environmental Research Center, Wetland and Aquatic Research Center

  October 5, 2022

  Exposure to 17α-ethinylestradiol results in differential susceptibility of largemouth bass (Micropterus salmoides) to bacterial infection

  Disease outbreaks, skin lesions, mortality events, and reproductive abnormalities have been observed in wild populations of centrarchids. The presence of estrogenic endocrine disrupting compounds (EEDCs) has been implicated as a potential causal factor for these effects. The effects of prior EEDC exposure on immune response were examined in juvenile largemouth bass (Micropterus salmoides) exposed

  Authors

  Jessica Kristin Leet, Justin Greer, Catherine A. Richter, Luke R. Iwanowicz, Edward Spinard, Jacquelyn McDonald, Carla M. Conway, Robert W. Gale, Donald E. Tillitt, John Hansen

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  Ecosystems Mission Area, Columbia Environmental Research Center, Eastern Ecological Science Center, Western Fisheries Research Center

  June 1, 2022

  Using structured decision making to evaluate potential management responses to detection of dreissenid mussel (Dreissena spp.) environmental DNA

  Environmental (e)DNA tools are sensitive and cost-effective for early detection of invasive species. However, the uncertainty associated with the interpretation of positive eDNA detections makes it challenging to determine appropriate natural resource management responses. Multiple sources of error can give rise to positive detections of eDNA in a sample when individuals of that species are not pr

  Authors

  Adam J. Sepulveda, David R. Smith, Katherine M O'Donnell, Nathan Owens, Brittany White, Catherine A. Richter, Christopher M. Merkes, Skylar Wolf, Mike Rau, Matthew Neilson, Wesley Daniel, Christine E. Dumoulin, Margaret Hunter

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  Ecosystems Mission Area, Columbia Environmental Research Center, Northern Rocky Mountain Science Center, Upper Midwest Environmental Sciences Center, Wetland and Aquatic Research Center

  March 18, 2022

  A comparison of eDNA and visual survey methods for detection of longnose darter Percina nasuta in Missouri

  The longnose darter Percina nasuta is a rare and cryptic fish that recently disappeared from much of its historic range. We developed and used an environmental DNA (eDNA) assay for longnose darter paired with visual surveys to better determine the species’ range and compare detection probability between sampling approaches in an occupancy modeling framework. We detected longnose darter eDNA furthe

  Authors

  Jacob Thomas Westhoff, Leah K. Berkman, Katy E. Klymus, Nathan Thompson, Catherine A. Richter

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  Ecosystems Mission Area, Columbia Environmental Research Center

  June 30, 2021

  Grass carp reproduction in small tributaries of Truman Reservoir, Missouri: Implications for establishment in novel habitats

  Substantial work has been conducted to estimate the river length required for recruitment of invasive Grass Carp Ctenopharyngodon idella and bigheaded carps (Bighead Carp Hypophthalmichthys nobilis and Silver Carp H. molitrix); however, the distance upstream and size of stream required for spawning remain unclear. Adult Grass Carp are regularly captured in Harry S. Truman Reservoir, Missouri, alth

  Authors

  Cari-Ann Hayer, Michael F. Bayless, Catherine A. Richter, Amy E. George, Duane Chapman

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  Ecosystems Mission Area, Contaminant Biology, Environmental Health Program, Toxic Substances Hydrology, Columbia Environmental Research Center

  March 4, 2021

  Reproduction

  Lake charr Salvelinus namaycush are typically fall spawners although one ecotype has populations that spawn during spring and fall (siscowets in Lake Superior). Lake charr are iteroparous (reproduce more than once in a lifetime) with group-synchronous ovarian development and typically spawn once per year. However, lake charr may not reproduce every year, a phenomenon known as skipped spawning. Fre

  Authors

  Frederick W. Goetz, J. Ellen Marsden, Catherine A. Richter, Donald E. Tillitt, Shawn P. Sitar, Stephen Riley, Charles C. Krueger

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  Ecosystems Mission Area, Columbia Environmental Research Center, Great Lakes Science Center

  November 26, 2020

  Metabarcoding assays for the detection of freshwater mussels (Unionida) with environmental DNA

  Freshwater mussels of the order Unionida are a widely distributed taxon that are important in maintaining freshwater ecosystems and are also highly imperiled throughout the world. Monitoring of mussel populations with environmental DNA (eDNA) is an attractive alternative to traditional methods because it is noninvasive and requires less labor and taxonomic knowledge from field personnel. We develo

  Authors

  Katy E. Klymus, Catherine A. Richter, Nathan Thompson, Jo Ellen Hinck, Jess W. Jones

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  Ecosystems Mission Area, Contaminant Biology, Environmental Health Program, Toxic Substances Hydrology, Columbia Environmental Research Center

  November 5, 2020

  Development and testing of species-specific quantitative PCR assays for environmental DNA applications

  New, non-invasive methods for detecting and monitoring species presence are being developed to aid in fisheries and wildlife conservation management. The use of environmental DNA (eDNA) samples for detecting macrobiota is one such group of methods that is rapidly becoming popular and being implemented in national management programs. Here we focus on the development of species-specific targeted as

  Authors

  Katy E. Klymus, Dannise Vannesa Ruiz Ramos, Nathan Thompson, Catherine A. Richter

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  Ecosystems Mission Area, Contaminant Biology, Environmental Health Program, Toxic Substances Hydrology, Columbia Environmental Research Center

  October 2, 2020

  Effects of early life stage exposure of largemouth bass to atrazine or a model estrogen (17α-ethinylestradiol)

  Endocrine disrupting contaminants are of continuing concern for potentially contributing to reproductive dysfunction in largemouth and smallmouth bass in the Chesapeake Bay watershed (CBW) and elsewhere. Exposures to atrazine (ATR) have been hypothesized to have estrogenic effects on vertebrate endocrine systems. The incidence of intersex in male smallmouth bass from some regions of CBW has been c

  Authors

  Jessica Kristin Leet, Catherine A. Richter, Robert S. Cornman, Jason P. Berninger, Ramji K. Bhandari, Diane K. Nicks, James L. Zajicek, Vicki S. Blazer, Donald E. Tillitt

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  Ecosystems Mission Area, Contaminant Biology, Environmental Health Program, Toxic Substances Hydrology, Columbia Environmental Research Center

  August 27, 2020

  Use of environmental DNA to detect grass carp spawning events

  The timing and location of spawning events are important data for managers seeking to control invasive grass carp populations. Ichthyoplankton tows for grass carp eggs and larvae can be used to detect spawning events; however, these samples can be highly debris-laden, and are expensive and laborious to process. An alternative method, environmental DNA (eDNA) technology, has proven effective in det

  Authors

  Cari-Ann Hayer, Michael F. Bayless, Amy E. George, Nathan Thompson, Catherine A. Richter, Duane Chapman

  By

  Ecosystems Mission Area, Columbia Environmental Research Center

  September 16, 2019

  Reporting the limits of detection and quantification for environmental DNA assays

  BackgroundEnvironmental DNA (eDNA) analysis is increasingly being used to detect the presence and relative abundance of rare species, especially invasive or imperiled aquatic species. The rapid progress in the eDNA field has resulted in numerous studies impacting conservation and management actions. However, standardization of eDNA methods and reporting across the field is yet to be fully establis

  Authors

  Katy E. Klymus, Christopher M. Merkes, Michael J. Allison, Caren S. Goldberg, Caren C. Helbing, Margaret Hunter, Craig Jackson, Richard F. Lance, Anna M. Mangan, Emy M. Monroe, Antoinette J. Piaggio, Joel P. Stokdyk, Chris C. Wilson, Catherine A. Richter

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  Ecosystems Mission Area, Wetland and Aquatic Research Center , Upper Midwest Environmental Sciences Center, Columbia Environmental Research Center

  Non-USGS Publications**

  Taylor, J.A., Richter, C.A., Suzuki, A., Watanabe, H., Iguchi, T., Coser, K.R., Shioda, T., and vom Saal, F.S., 2012, Dose-related estrogen effects on gene expression in fetal mouse prostate mesenchymal cells: PLOS ONE, v. 7, no. 10, p. e48311
  

  Taylor, J.A., Richter, C.A., Ruhlen, R.L., and vom Saal, F.S., 2011, Estrogenic environmental chemicals and drugs: Mechanisms for effects on the developing male urogenital system: The Journal of Steroid Biochemistry and Molecular Biology, v. 127, no. 1, p. 83-95
  

  Richter, C.A., Taylor, J.A., Ruhlen, R.L., Welshons, W.V., and vom Saal, F.S., 2007, Estradiol and Bisphenol A stimulate androgen receptor and estrogen receptor gene expression in fetal mouse prostate mesenchyme cells: Environmental Health Perspectives, v. 115, no. 6, p. 902-908

  vom Saal, F.S., Richter, C.A., Mao, J., and Welshons, W.V., 2005, Commercial animal feed: Variability in estrogenic activity and effects on body weight in mice: Birth Defects Research Part A: Clinical and Molecular Teratology, v. 73, no. 7, p. 474-475
  

  Timms, B.G., Howdeshell, K.L., Barton, L., Bradley, S., Richter, C.A., and vom Saal, F.S., 2005, Estrogenic chemicals in plastic and oral contraceptives disrupt development of the fetal mouse prostate and urethra: Proceedings of the National Academy of Sciences of the United States of America, v. 102, no. 19, p. 7014-7019
  

  Richter, C., Timms, B., and vom Saal, F., 2005, Prostate development: mechanisms for opposite effects of low and high doses of estrogenic chemicals, in Naz, R., ed., Endocrine Disruptors: Effects on Male and Female Reproductive Systems: New York, CRC Press, p. 379-410.
  

  vom Saal, F.S., Richter, C.A., Ruhlen, R.R., Nagel, S.C., Timms, B.G., and Welshons, W.V., 2005, The importance of appropriate controls, animal feed, and animal models in interpreting results from low-dose studies of bisphenol A: Birth Defects Research Part A: Clinical and Molecular Teratology, v. 73, no. 3, p. 140-145
  

  vom Saal, F.S., Richter, C.A., Ruhlen, R.R., Nagel, S.C., and Welshons, W.V., 2004, Disruption of laboratory experiments due to leaching of Bisphenol A from polycarbonate cages and bottles and uncontrolled variability in components of animal feed, in National Research Council, ed., The Development of Science-based Guidelines for Laboratory Animal Care: Proceedings of the November 2003 International Workshop: The National Academies Press, p. 65-69.
  

  **Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.