The data were gathered as part of a long-term, seasonal assessment of estrogenicity in selected locations within Pennsylvania from 2012-2017. Data were collected over this time frame to determine seasonal and annual changes in surface water estrogenicity.Estrogen is reported as estrogen equivalents (EEQ) as determined by a bioluminescent yeast estrogen screen. A total of 193 grab water samples were collected during this time of which 41 percent had EEQ above the limit of detection. The maximum estrogen equivalents (EEQ) measured was 5.44 ng EEQ/ L, while the median concentration was 0.44 ng/L. POCIS samplers were deployed at a number of sites to determine time-weighted estrogenicity between 2014 and 2017. A total of 150 POCIS samples were deployed over this time of which 77 percent had EEQ above the limit of detection.. The maximum estrogen equivalents (EEQ) measured was 12 ng EEQ/ POCIS.