The dataset includes Roundtail Chub and Flannelmouth Sucker detections at passive integrated transponder (PIT) tag antenna systems. We installed nine instream Biomark PIT-tag antenna systems (five 24-meter and four 9-meter antennas) in a pass-over orientation throughout the Blacks Fork subbasin. In addition to the fixed antennas, we used a Biomark inflatable antenna and two Biomark submersible PIT-tag antenna systems to increase detections.