Dataset containing information for white-tailed deer samples from Minnesota. Samples were genotyped for 11 microsatellites markers and sequenced for a fragment of approx. 546 bp belonging to the mitochondrial control region. Marker OvirQ should not be used as it presents alleles inconsistent with reported pattern, with some alleles separated by only 1 base pair and inconsistent between runs. Coordinates representing the centroid of Township-range-section of origin are given as UTM 15N.