Low genetic differentiation across restored and natural populations shortly after a large-scale, post-fire seeding in the Great Basin

Genetic diversity is essential for species to adapt to environmental changes. In restoration efforts, such as those after large wildfires in the sagebrush steppe of the Great Basin, commercially produced native seeds are used to revegetate the burned areas. While native seed certification and procurement protocols assure that genetically appropriate seeds are used for restoration, how post-fire seeding affects the genetic integrity of wildland populations at the landscape scale is unknown. To assess the effects of seeding on the genetic diversity of wildland populations, we conducted a genetic survey of Bluebunch wheatgrass (Pseudoroegneria spicata [Pursh] Á.Löve ssp. Spicata) populations within the perimeter of a recent megafire in southeastern Oregon and southwestern Idaho, United States. We genotyped 760 samples with 10 polymorphic loci. We found similar genetic diversity in populations four to 5 years after seeding compared to unseeded populations that were either burned or unburned. Furthermore, genetic diversity neither increased nor decreased with distance from the fire's edge, suggesting that wind dispersal from neighboring remnant populations plays a minor role in immediate post-fire recovery compared to resprouting and germination from the seed bank. Though no change was detected in the short term, this survey of genetic variation after a post-fire seeding provides an empirical baseline that can be used to track changes in genetic diversity of these wildland populations over time.