Publications
Below is a list of available Fish Health Program peer reviewed and published science.
Filter Total Items: 323
Myxobolus cerebralis in native cutthroat trout of the Yellowstone Lake ecosystem Myxobolus cerebralis in native cutthroat trout of the Yellowstone Lake ecosystem
The exotic parasite Myxobolus cerebralis was first detected in native adult Yellowstone cutthroat trout Oncorhynchus clarkii bouvierii from Yellowstone Lake in 1998, seriously threatening the ecological integrity of this pristine, naturally functioning ecosystem. We immediately began to assess the prevalence and spatial extent of M. cerebralis infection in Yellowstone cutthroat trout...
Authors
T.M. Koel, D.L. Mahony, K. Kinnan, C. Rasmussen, C. Hudson, S. Murcia, B.L. Kerans
Isolation of viral haemorrhagic septicaemia virus from muskellunge, Esox masquinongy (Mitchill), in Lake St Clair, Michigan, USA reveals a new sublineage of the North American genotype Isolation of viral haemorrhagic septicaemia virus from muskellunge, Esox masquinongy (Mitchill), in Lake St Clair, Michigan, USA reveals a new sublineage of the North American genotype
Viral haemorrhagic septicaemia virus (VHSV) was isolated from muskellunge, Esox masquinongy (Mitchill), caught from the NW portion of Lake St Clair, Michigan, USA in 2003. Affected fish exhibited congestion of internal organs; the inner wall of the swim bladder was thickened and contained numerous budding, fluid-filled vesicles. A virus was isolated using fish cell lines inoculated with...
Authors
E. Elsayed, M. Faisal, M. Thomas, G. Whelan, W. Batts, J. Winton
Introduction of translation stop codons into the viral glycoprotein gene in a fish DNA vaccine eliminates induction of protective immunity Introduction of translation stop codons into the viral glycoprotein gene in a fish DNA vaccine eliminates induction of protective immunity
A highly efficacious DNA vaccine against a fish rhabdovirus, infectious hematopoietic necrosis virus (IHNV), was mutated to introduce two stop codons to prevent glycoprotein translation while maintaining the plasmid DNA integrity and RNA transcription ability. The mutated plasmid vaccine, denoted pIHNw-G2stop, when injected intramuscularly into fish at high doses, lacked detectable...
Authors
K.A. Garver, C. Conway, Gael Kurath
An examination of environmental factors associated with Myxobolus cerebralis infection of wild trout in Pennsylvania An examination of environmental factors associated with Myxobolus cerebralis infection of wild trout in Pennsylvania
Salmonid whirling disease, caused by the myxosporean parasite Myxobolus cerebralis, was first observed in the United States in 1956 in central Pennsylvania. The parasite was subsequently discovered at several culture facilities throughout the state, and widespread distribution of this parasite via the stocking of subclinically infected brook trout Salvelinus fontinalis, rainbow trout...
Authors
Adam Kaeser, Charlotte Rasmussen, William Sharpe
Identification and regulatory analysis of rainbow trout tapasin and tapasin-related genes Identification and regulatory analysis of rainbow trout tapasin and tapasin-related genes
Tapasin (TAPBP) is a key member of MHC class Ia antigen-loading complexes, bridging the class Ia molecule to the transporter associated with antigen presentation (TAP). As part of an ongoing study of MHC genomics in rainbow trout, we have identified two rainbow trout TAPBP genes (Onmy-TAPBP.a and .b) and a similar but distinct TAPBP-related gene (Onmy-TAPBP-R) that had previously only...
Authors
E.D. Landis, Y. Palti, J. Dekoning, R. Drew, R.B. Phillips, J.D. Hansen
Risk of Myxobolus cerebralis infection to rainbow trout in the Madison River, Montana, USA Risk of Myxobolus cerebralis infection to rainbow trout in the Madison River, Montana, USA
Myxobolus cerebralis, the parasite that causes salmonid whirling disease, has had detrimental effects on several salmonid populations in the Intermountain West, including the rainbow trout in the Madison River, Montana, USA. The goal of this study was to examine relationships among characteristics of the environment, Tubifex tubifex (the alternate host) populations, and rainbow trout...
Authors
R.C. Krueger, B.L. Kerans, E.R. Vincent, C. Rasmussen
Genetic effects of ELISA-based segregation for control of bacterial kidney disease in Chinook salmon (Oncorhynchus tshawytscha) Genetic effects of ELISA-based segregation for control of bacterial kidney disease in Chinook salmon (Oncorhynchus tshawytscha)
We evaluated genetic variation in ability of Chinook salmon (Oncorhynchus tshawytscha) to resist two bacterial pathogens: Renibacterium salmoninarum, the agent of bacterial kidney disease (BKD), and Listonella anguillarum, an agent of vibriosis. After measuring R. salmoninarum antigen in 499 adults by enzyme-linked immunosorbent assay (ELISA), we mated each of 12 males with high or low...
Authors
J.J. Hard, D.G. Elliott, R.J. Pascho, D.M. Chase, L.K. Park, J. R. Winton, D.E. Campton
Evolution of the CD4 family: teleost fish possess two divergent forms of CD4 in addition to lymphocyte activation gene-3 Evolution of the CD4 family: teleost fish possess two divergent forms of CD4 in addition to lymphocyte activation gene-3
The T cell coreceptor CD4 is a transmembrane glycoprotein belonging to the Ig superfamily and is essential for cell-mediated immunity. Two different genes were identified in rainbow trout that resemble mammalian CD4. One (trout CD4) encodes four extracellular Ig domains reminiscent off mammalian CD4, whereas the other (CD4REL) codes for two Ig domains. Structural motifs within the amino...
Authors
K.J. Laing, J.J. Zou, M. Purcell, R. Phillips, C.J. Secombes, J.D. Hansen
Comprehensive gene expression profiling following DNA vaccination of rainbow trout against infectious hematopoietic necrosis virus Comprehensive gene expression profiling following DNA vaccination of rainbow trout against infectious hematopoietic necrosis virus
The DNA vaccine based on the glycoprotein gene of Infectious hematopoietic necrosis virus induces a non-specific anti-viral immune response and long-term specific immunity against IHNV. This study characterized gene expression responses associated with the early anti-viral response. Homozygous rainbow trout were injected intra-muscularly (I.M.) with vector DNA or the IHNV DNA vaccine...
Authors
Maureen K. Purcell, Krista Nichols, James Winton, Gael Kurath, Gary Thorgaard, Paul Wheeler, John Hansen, Russell Herwig, Linda Park
Guide to the identification of fish protozoan and metazoan parasites in stained tissue sections Guide to the identification of fish protozoan and metazoan parasites in stained tissue sections
The identification of protozoan and metazoan parasites is traditionally carried out using a series of classical keys based upon the morphology of the whole organism. However, in stained tissue sections prepared for light microscopy, taxonomic features will be missing, thus making parasite identification difficult. This work highlights the characteristic features of representative...
Authors
D.W. Bruno, B. Nowak, D.G. Elliott
Sequence motifs and prokaryotic expression of the reptilian paramyxovirus fusion protein Sequence motifs and prokaryotic expression of the reptilian paramyxovirus fusion protein
Fourteen reptilian paramyxovirus isolates were chosen to represent the known extent of genetic diversity among this novel group of viruses. Selected regions of the fusion (F) gene were sequenced, analyzed and compared. The F gene of all isolates contained conserved motifs homologous to those described for other members of the family Paramyxoviridae including: signal peptide...
Authors
J. Franke, W.N. Batts, W. Ahne, Gael Kurath, J. R. Winton