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Capture of environmental DNA (eDNA) from water samples by flocculation

May 29, 2020

The analysis of environmental DNA (eDNA) has become a widely used approach to problem solving in species management. The detection of cryptic species including invasive and (or) species at risk is the goal, typically accomplished by testing water and sediment for the presence of characteristic DNA signatures. Reliable and efficient procedures for the capture of eDNA are required, especially those that can be performed easily in the field by personnel with limited training and citizen scientists. The capture of eDNA using membrane filtration is widely used currently. This approach has inherent issues that include the choice of filter material and porosity, filter fouling, and time required on site for the process to be performed. Flocculation offers an alternative that can be easily implemented and applied to sampling regimes that strive to cover broad territories in limited time.

Citation Information

Publication Year 2020
Title Capture of environmental DNA (eDNA) from water samples by flocculation
DOI 10.3791/60967
Authors W. Bane Schill
Publication Type Article
Publication Subtype Journal Article
Series Title JOVE Journal Of Visualized Experiments
Series Number
Index ID 70211652
Record Source USGS Publications Warehouse
USGS Organization Leetown Science Center