Determination of Antimycin-A in water by liquid chromatographic/mass spectrometry: single-laboratory validation
March 1, 2013
An LC/MS method was developed and validated for the quantitative determination and confirmation of antimycin-A (ANT-A) in water from lakes or streams. Three different water sample volumes (25, 50, and 250 mL) were evaluated. ANT-A was stabilized in the field by immediately extracting it from water into anhydrous acetone using SPE. The stabilized concentrated samples were then transported to a laboratory and analyzed by LC/MS using negative electrospray ionization. The method was determined to have adequate accuracy (78 to 113% recovery), precision (0.77 to 7.5% RSD with samples ≥500 ng/L and 4.8 to 17% RSD with samples ≤100 ng/L), linearity, and robustness over an LOQ range from 8 to 51 600 ng/L.
Citation Information
Publication Year | 2013 |
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Title | Determination of Antimycin-A in water by liquid chromatographic/mass spectrometry: single-laboratory validation |
DOI | 10.5740/jaoacint.12-286 |
Authors | Jeffry A. Bernardy, Terrance D. Hubert, Jacob M. Ogorek, Larry J. Schmidt |
Publication Type | Article |
Publication Subtype | Journal Article |
Series Title | Journal of AOAC International |
Index ID | 70158936 |
Record Source | USGS Publications Warehouse |
USGS Organization | Upper Midwest Environmental Sciences Center |