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Identification of a genetic marker that discriminates ocean-type and stream-type chinook salmon in the Columbia River basin

January 1, 2003

A marker based on randomly amplified polymorphic DNA (RAPD), OT-38, was discovered that nonlethally discriminates between stream-type and ocean-type populations of chinook salmon Oncorhynchus tshawytscha in the Columbia River basin, including the threatened fall-run (ocean-type) and spring-run (stream-type) Snake River populations. This marker was developed by amplifying chinook salmon genomic DNA with a single RAPD primer, sequencing the termini of the polymorphic products, and designing primer pairs for allele-specific amplification. It was used to assay 18-80 individuals from several wild and hatchery populations differing in year-class, freshwater life history, and location along the Columbia River OT-38 unambiguously distinguished ocean-type from stream-type populations in 93.1% of the chinook salmon sampled.

Citation Information

Publication Year 2003
Title Identification of a genetic marker that discriminates ocean-type and stream-type chinook salmon in the Columbia River basin
DOI 10.1577/1548-8659(2003)132<0131:IOAGMT>2.0.CO;2
Authors C. Rasmussen, C.O. Ostberg, D.R. Clifton, J.L. Holloway, R. J. Rodriguez
Publication Type Article
Publication Subtype Journal Article
Series Title Transactions of the American Fisheries Society
Index ID 70026232
Record Source USGS Publications Warehouse
USGS Organization Western Fisheries Research Center