A marker based on randomly amplified polymorphic DNA (RAPD), OT-38, was discovered that nonlethally discriminates between stream-type and ocean-type populations of chinook salmon Oncorhynchus tshawytscha in the Columbia River basin, including the threatened fall-run (ocean-type) and spring-run (stream-type) Snake River populations. This marker was developed by amplifying chinook salmon genomic DNA with a single RAPD primer, sequencing the termini of the polymorphic products, and designing primer pairs for allele-specific amplification. It was used to assay 18-80 individuals from several wild and hatchery populations differing in year-class, freshwater life history, and location along the Columbia River OT-38 unambiguously distinguished ocean-type from stream-type populations in 93.1% of the chinook salmon sampled.
|Title||Identification of a genetic marker that discriminates ocean-type and stream-type chinook salmon in the Columbia River basin|
|Authors||C. Rasmussen, C.O. Ostberg, D.R. Clifton, J.L. Holloway, R. J. Rodriguez|
|Publication Subtype||Journal Article|
|Series Title||Transactions of the American Fisheries Society|
|Record Source||USGS Publications Warehouse|
|USGS Organization||Western Fisheries Research Center|