Skip to main content
U.S. flag

An official website of the United States government

Isolation of anaerobic oxalate-degrading bacteria from freshwater lake sediments

January 1, 1985

Enrichment cultures that anaerobically degraded oxalate were obtained from lake sediment inocula. From these, 5 pure cultures of anaerobic oxalate-degrading bacteria were isolated and partially characterized. The isolates were Gram-negative, non-sporeforming, non-motile, obligate anaerobes. Oxalate was required for growth and was stoichiometrically converted to formate; 14CO2 was also recovered when 14C-oxalate was added. Maximal growth occurred when the oxalate concentration was 50 mM. Acetate stimulated growth in the presence of oxalate, however, 14C-experiments indicated that acetate was only utilized for cell carbon.

The isolates were either spiral-shaped or rod-shaped organisms. The first morphotype grew much more slowly than the second and exhibited 13-fold lower cell yields. These isolates represent a new strain of oxalate-degrading bacteria. The second morphotype was similar to the anaerobic oxalate-degrading bacteria previously found in rumen. This report extends the known habitats in which anaerobic oxalate-degrading organisms have been found to include aquatic sediments.

Publication Year 1985
Title Isolation of anaerobic oxalate-degrading bacteria from freshwater lake sediments
DOI 10.1007/BF00446732
Authors R. L. Smith, F.E. Strohmaier, Ronald S. Oremland
Publication Type Article
Publication Subtype Journal Article
Series Title Archives of Microbiology
Index ID 70013267
Record Source USGS Publications Warehouse
USGS Organization Toxic Substances Hydrology Program