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Novel microsatellite loci for studies of Thamnophis Gartersnake genetic identity and hybridization

June 1, 2012

Butler’s Gartersnakes (BGS; Thamnophis butleri) are confined to open and semi-open canopy wetlands and adjacent uplands, habitats under threat of development in Wisconsin. To address issues of species identity and putative hybridization with congeneric snakes, a suite of 18 microsatellite loci capable of cross-species amplification of Plains Gartersnakes (T. radix) and Common Gartersnakes (T. sirtalis) was developed. All loci were polymorphic in BGS with mean number of alleles per locus of 16.11 (range = 3–41) and mean observed heterozygosity of 0.659 (range = 0.311–0.978). Loci amplified efficiently in the congeneric species with high levels of intra- and inter-specific variation. These loci will aid ongoing efforts to effectively identify and manage BGS in Wisconsin.

Citation Information

Publication Year 2012
Title Novel microsatellite loci for studies of Thamnophis Gartersnake genetic identity and hybridization
DOI 10.1007/s12686-011-9555-8
Authors Brian L. Sloss, Gregor W. Schuurman, Rori A. Paloski, Owen D. Boyle, Joshua M. Kapfer
Publication Type Article
Publication Subtype Journal Article
Series Title Conservation Genetics Resources
Series Number
Index ID 70187479
Record Source USGS Publications Warehouse
USGS Organization Coop Res Unit Leetown