Butler’s Gartersnakes (BGS; Thamnophis butleri) are confined to open and semi-open canopy wetlands and adjacent uplands, habitats under threat of development in Wisconsin. To address issues of species identity and putative hybridization with congeneric snakes, a suite of 18 microsatellite loci capable of cross-species amplification of Plains Gartersnakes (T. radix) and Common Gartersnakes (T. sirtalis) was developed. All loci were polymorphic in BGS with mean number of alleles per locus of 16.11 (range = 3–41) and mean observed heterozygosity of 0.659 (range = 0.311–0.978). Loci amplified efficiently in the congeneric species with high levels of intra- and inter-specific variation. These loci will aid ongoing efforts to effectively identify and manage BGS in Wisconsin.
|Title||Novel microsatellite loci for studies of Thamnophis Gartersnake genetic identity and hybridization|
|Authors||Brian L. Sloss, Gregor W. Schuurman, Rori A. Paloski, Owen D. Boyle, Joshua M. Kapfer|
|Publication Subtype||Journal Article|
|Series Title||Conservation Genetics Resources|
|Record Source||USGS Publications Warehouse|
|USGS Organization||Coop Res Unit Leetown|