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Physio-chemical and morphological characteristics of avian encephalomyelitis virus

January 19, 2016

Avian encephalomyelitis virus (AEV) was purified from infected chick embryos by a gradient centrifugation in cesium chloride. The virus had a buoyant density of 1.31 to 1.32 g/ml and a sedimentation coefficient of 148 S. The purified AEV was resistant to treatments with chloroform, acid pH or trypsin. The presence of Mg++ stabilized the virus against heat inactivation (56°C, 1 h). Electron microscopic study showed the virus to be 24 to 32 nm in diameter. The surface structure of the purified virus was not easily discernable. Nevertheless, with uranyl acetate-stained particles, Markham's rotation technique revealed that AEV has five-fold symmetry with 32 or 42 capsomers. Exact classification of AEV awaits characterization of the viral nucleic acid.

Citation Information

Publication Year 1980
Title Physio-chemical and morphological characteristics of avian encephalomyelitis virus
DOI 10.1016/0378-1135(80)90013-9
Authors L.H. Gosting, B.W. Grinnell, M. Matsumoto
Publication Type Article
Publication Subtype Journal Article
Series Title Veterinary Microbiology
Series Number
Index ID 70162230
Record Source USGS Publications Warehouse
USGS Organization Western Fisheries Research Center