A rapid and efficient assay for extracting DNA from fungi
Aims: A method for the rapid extraction of fungal DNA from small quantities of tissue in a batch-processing format was investigated. Methods and Results: Tissue (< 3.0 mg) was scraped from freshly-grown fungal isolates. The tissue was suspended in buffer AP1 and subjected to seven rounds of freeze/thaw using a crushed dry ice/ethanol bath and a boiling water bath. After a 30 min boiling step, the tissue was quickly ground against the wall of the microfuge tube using a sterile pipette tip. The Qiagen DNeasy Plant Tissue Kit protocol was then used to purify the DNA for PCR/ sequencing applications. Conclusions: The method allowed batch DNA extraction from multiple fungal isolates using a simple yet rapid and reliable assay. Significance and Impact of the Study: Use of this assay will allow researchers to obtain DNA from fungi quickly for use in molecular assays that previously required specialized instrumentation, was time-consuming or was not conducive to batch processing.
Citation Information
Publication Year | 2002 |
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Title | A rapid and efficient assay for extracting DNA from fungi |
DOI | 10.1046/j.1472-765x.2002.01071.x |
Authors | Dale W. Griffin, C.A. Kellogg, K.K. Peak, E.A. Shinn |
Publication Type | Article |
Publication Subtype | Journal Article |
Series Title | Letters in Applied Microbiology |
Index ID | 70024795 |
Record Source | USGS Publications Warehouse |