Riboflavin requirement of fingerling rainbow trout

Fingerling rainbow trout (Salmo gairdneri) were fed purified diets containing graded levels (0, 3, 6, 12, 18, 24, and 54 mg/kg) of supplemental riboflavin for 20 weeks. The cellular riboflavin supply of the fingerlings was estimated on the basis of the activity of the FAD‐dependent enzyme, erythrocyte glutathione reductase (EGR). The dietary riboflavin level that produced the fastest growth, an efficient feed: gain ratio, and normal values for the EGR activity coefficient was about 3 mg of supplemental riboflavin per kilogram of diet.