Water samples were collected in the San Francisco Bay estuary during 22 cruises from January through December 1993. Conductivity, temperature, light attenuation, turbidity, oxygen, and in-vivo fluorescence were measured 1ongitudinally and vertically in the main channel of the estuary from south of the Dumbarton Bridge in the southern part of the bay to Rio Vista on the Sacramento River. Discrete water samples were analyzed for chlorophyll a, phaeopigments, suspended particulate matter, and dissolved oxygen. Water density was calculated from values for salinity, temperature, and pressure (depth), and is included in the data summaries. Photosynthetic parameters, water column respiration, and phytoplankton species abundance and cell volume were determined at selected stations every other month.