We exposed 3 benthic invertebrates, the clam Macoma balthica, the polychaete Neanthes arenaceodentataand the amphipod Leptocheirus plumulosus, to Ag-contaminated sediments to evaluate the relative importance of various uptake routes (sediments, porewater or overlying water, and supplementary food) for Ag bioaccumulation. Silver bioaccumulation was evaluated at 4 levels of sediment Ag (0.1, 0,3, 1,2 and 3.3 µmol Ag g-1) and 2 levels of acid-volatile sulfide (AVS),