Carl Ostberg, Ph.D.
Carl is a Research Fish Biologist at the Western Fisheries Research Center.
Research Interests:
Carl’s research interests focus on evolutionary and conservation genetics. Current research projects include several studies directed at generating a greater understanding of intrinsic and extrinsic factors associated with hybridization between rainbow and cutthroat trout, using next generation sequencing to develop species-specific markers that differentiate endangered suckers endemic to the Klamath Lake basin, developing eDNA tool to evaluate recolonization extent of native and non-native fishes in the Elwha River following dam removal, and investigating morphological and genetic diversity of Dolly Varden in the Russian Far East.
Professional Experience
2008 to Present - Research Fish Biologist, U.S. Geological Survey, Western Fisheries Research Center, Seattle, WA
2004-2008 - Fishery Biologist, U.S. Geological Survey, Western Fisheries Research Center, Seattle, WA
1997-2004 - Laboratory Technician, U.S. Geological Survey, Western Fisheries Research Center, Seattle, WA
1995-1997 - Graduate Teaching Assistant, Department of Zoology, Washington State University, Pullman, WA
Education and Certifications
Ph.D. 2014. University of Washington, School of Aquatic and Fishery Sciences, Seattle, WA
M.S. 1997. Washington State University, Zoology, Pullman, WA
B.S. 1989. Washington State University, Biology, Pullman, WA
Affiliations and Memberships*
American Fisheries Society, Genetics section
Science and Products
Filter Total Items: 17
Environmental DNA survey results for Oncorhynchus mykiss and Cottus aleuticus in coastal streams of Big Sur, California, 2021-2022 Environmental DNA survey results for Oncorhynchus mykiss and Cottus aleuticus in coastal streams of Big Sur, California, 2021-2022
The data support a study that surveyed the spatial distribution of Oncorhynchus mykiss and Cottus aleuticus eDNA in coastal streams of Big Sur, California, 2021-2022 following post-fire debris flows. The metadata represent qPCR quantification cycle (Cq) values for O. mykiss and C. aleuticus assays performed on water samples collected during June and July of 2021 and 2022 from the...
Upper Skagit Reservoir Food Web Data, 2005-2021 Upper Skagit Reservoir Food Web Data, 2005-2021
This release includes data used to evaluate the structure and function of the Ross Lake and Diablo Lake food webs. This includes data on zooplankton density and production (zooplankton_density.csv and daphnia_region_production_biomass.csv), lake volume estimates used to expand zooplankton density and production data (lake_volume_estimates.csv), fish sampling (FishSampleEvents.csv), fish...
Data relating to the development of a quantitative PCR assay for detecting Northwest salamander (Ambystoma gracile) in environmental DNA samples Data relating to the development of a quantitative PCR assay for detecting Northwest salamander (Ambystoma gracile) in environmental DNA samples
The data support a study that describes the development and validation of a primer and probe based quantitative PCR (qPCR) assay for use with environmental DNA to detect Northwest salamander (Ambystoma gracile), a species endemic to the temperate Pacific coastal region of North America. The metadata includes qPCR quantification cycle (Cq) values from testing the A. gracile assay on DNA...
Spatial and temporal surveys of salmon eDNA in Seattle urban creeks, Washington, 2018 - 2020 Spatial and temporal surveys of salmon eDNA in Seattle urban creeks, Washington, 2018 - 2020
The data support a study that surveyed the spatial and temporal distribution of salmon eDNA in Seattle urban creeks, Washington, 2018 - 2020. The metadata represent qPCR quantification cycle (Cq) values for Chinook salmon, coho salmon, and coastal cutthroat trout assays performed on water samples collected on specific days at specific sites on Thornton Creek, Taylor Creek, and Mapes...
Information tables associated with a risk assessment for bull trout introduction into Sullivan Lake, northeastern, Washington including population donor sources and resident species Information tables associated with a risk assessment for bull trout introduction into Sullivan Lake, northeastern, Washington including population donor sources and resident species
Introduction and translocation programs require thoughtful planning to increase the likelihood of success and to understand the benefits, risks, and constraints of such programs. A risk assessment was completed for bull trout introduction into the Sullivan Lake and Harvey Creek watershed, northeastern Washington. The risk assessment was designed to evaluate potential risks to resident...
Spatial and temporal survey of waterborne myxozoan parasites in the Lake Sammamish watershed, Washington, from 2019 - 2021 Spatial and temporal survey of waterborne myxozoan parasites in the Lake Sammamish watershed, Washington, from 2019 - 2021
There is a fundamental knowledge gap on the distribution, prevalence, intensity, and ecology of salmonid myxozoan parasites in the Lake Sammamish watershed, Washington. To address this knowledge gap, we tested water samples for Ceratonova shasta, Parvicapsula minibicornis and Tetracapsuloides bryosalmonae DNA from 84 sites distributed throughout the Lake Sammamish watershed in fall 2019...
Filter Total Items: 41
Development of a quantitative PCR assay for detecting northwest salamander (ambystoma gracile) in environmental DNA samples Development of a quantitative PCR assay for detecting northwest salamander (ambystoma gracile) in environmental DNA samples
We developed a primer and probe based quantitative PCR assay for use with environmental DNA to detect Northwest salamander (Ambystoma gracile), a species endemic to the temperate Pacific coastal region of North America. The assay targets a region in the mitochondrial DNA (mtDNA) D-loop gene. Tests of the assay were performed in silico (using the NCBI BLAST tool), in vitro (using DNA...
Authors
Marshal S. Hoy, Carl O. Ostberg
Risk assessment for bull trout introduction into Sullivan Lake and Harvey Creek, northeastern Washington Risk assessment for bull trout introduction into Sullivan Lake and Harvey Creek, northeastern Washington
The Kalispel Tribe of Indians (KT), U. S. Fish and Wildlife Service, and Washington Department of Fish and Wildlife are engaged in conservation of bull trout (Salvelinus confluentus) in the Lake Pend Oreille (LPO) Core Area. The LPO is a complex habitat core area which falls within three states (Montana, Idaho, and Washington) and a tribal entity. As part of the conservation process, KT...
Authors
Jill M. Hardiman, Rachel B. Breyta, Carl O. Ostberg
Growth, survival, and cohort formation of juvenile Lost River (Deltistes luxatus) and shortnose suckers (Chasmistes brevirostris) in Upper Klamath Lake, Oregon, and Clear Lake Reservoir, California—2019 Monitoring Report Growth, survival, and cohort formation of juvenile Lost River (Deltistes luxatus) and shortnose suckers (Chasmistes brevirostris) in Upper Klamath Lake, Oregon, and Clear Lake Reservoir, California—2019 Monitoring Report
Populations of federally endangered Lost River (Deltistes luxatus) and shortnose suckers (Chasmistes brevirostris) in Upper Klamath Lake, Oregon, and Clear Lake Reservoir (hereinafter, Clear Lake), California, are experiencing long-term decreases in abundance. Upper Klamath Lake populations are decreasing not only due to adult mortality, which is relatively low, but also because they are...
Authors
Ryan J. Bart, Caylen M. Kelsey, Summer M. Burdick, Marshal S. Hoy, Carl O. Ostberg
Ontogeny of eDNA shedding during early development in Chinook Salmon (Oncorhynchus tshawytscha) Ontogeny of eDNA shedding during early development in Chinook Salmon (Oncorhynchus tshawytscha)
Knowledge of the timing of major life history events in aquatic species is important for informing conservation and resource management planning. Accordingly, surveys of environmental DNA (eDNA) have been performed to determine the efficacy of eDNA for providing information on life history events, primarily focusing on the timing of events associated with spawning, and these studies have...
Authors
Carl O. Ostberg, Dorothy M. Chase
Survival and growth of suckers in mesocosms at three locations within Upper Klamath Lake, Oregon, 2018 Survival and growth of suckers in mesocosms at three locations within Upper Klamath Lake, Oregon, 2018
Executive Summary Due to high mortality in the first year or two of life, Lost River (Deltistes luxatus sp.) and Shortnose suckers (Chasmistes brevirostris sp.) in Upper Klamath Lake, Oregon rarely reach maturity. In 2015, the U.S. Fish and Wildlife Service began the Sucker Assisted Rearing Program (SARP) to improve early life survival before releasing the fish back into Upper Klamath...
Authors
Summer M. Burdick, Carla M. Conway, Carl O. Ostberg, Ryan J. Bart, Diane G. Elliott
Environmental DNA is an effective tool to track recolonizing migratory fish following large‐scale dam removal Environmental DNA is an effective tool to track recolonizing migratory fish following large‐scale dam removal
Environmental DNA (eDNA) has emerged as a potentially powerful tool for use in conservation and resource management, including for tracking the recolonization dynamics of fish populations. We used eDNA to assess the effectiveness of dam removal to restore fish passage on the Elwha River in Washington State (USA). Using a suite of 11 species‐specific eDNA polymerase chain reaction (PCR)...
Authors
Jeffrey J. Duda, Marshal S. Hoy, Dorothy M. Chase, George R. Pess, Samuel J. Brenkman, Michael M McHenry, Carl O. Ostberg
Non-USGS Publications**
Ristow, S.S., L.D. Grabowski, C.O. Ostberg, B. Robison, and G.H. Thorgaard. 1998. Development of long-term cell lines from homozygous clones of rainbow trout. Journal of Aquatic Animal Health 10(1): 75-82. DOI: 10.1577/1548-8667(1998)010<0075:DOLTCL>2.0.CO;2.
Peek, A.S., P.A. Wheeler, C.O. Ostberg, and G.H. Thorgaard. 1997. A minichromosome carrying a pigmentation gene and brook trout DNA sequences in transgenic rainbow trout. Genome 40(5): 594-599. DOI: 10.1139/g97-778.
**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
Science and Products
Filter Total Items: 17
Environmental DNA survey results for Oncorhynchus mykiss and Cottus aleuticus in coastal streams of Big Sur, California, 2021-2022 Environmental DNA survey results for Oncorhynchus mykiss and Cottus aleuticus in coastal streams of Big Sur, California, 2021-2022
The data support a study that surveyed the spatial distribution of Oncorhynchus mykiss and Cottus aleuticus eDNA in coastal streams of Big Sur, California, 2021-2022 following post-fire debris flows. The metadata represent qPCR quantification cycle (Cq) values for O. mykiss and C. aleuticus assays performed on water samples collected during June and July of 2021 and 2022 from the...
Upper Skagit Reservoir Food Web Data, 2005-2021 Upper Skagit Reservoir Food Web Data, 2005-2021
This release includes data used to evaluate the structure and function of the Ross Lake and Diablo Lake food webs. This includes data on zooplankton density and production (zooplankton_density.csv and daphnia_region_production_biomass.csv), lake volume estimates used to expand zooplankton density and production data (lake_volume_estimates.csv), fish sampling (FishSampleEvents.csv), fish...
Data relating to the development of a quantitative PCR assay for detecting Northwest salamander (Ambystoma gracile) in environmental DNA samples Data relating to the development of a quantitative PCR assay for detecting Northwest salamander (Ambystoma gracile) in environmental DNA samples
The data support a study that describes the development and validation of a primer and probe based quantitative PCR (qPCR) assay for use with environmental DNA to detect Northwest salamander (Ambystoma gracile), a species endemic to the temperate Pacific coastal region of North America. The metadata includes qPCR quantification cycle (Cq) values from testing the A. gracile assay on DNA...
Spatial and temporal surveys of salmon eDNA in Seattle urban creeks, Washington, 2018 - 2020 Spatial and temporal surveys of salmon eDNA in Seattle urban creeks, Washington, 2018 - 2020
The data support a study that surveyed the spatial and temporal distribution of salmon eDNA in Seattle urban creeks, Washington, 2018 - 2020. The metadata represent qPCR quantification cycle (Cq) values for Chinook salmon, coho salmon, and coastal cutthroat trout assays performed on water samples collected on specific days at specific sites on Thornton Creek, Taylor Creek, and Mapes...
Information tables associated with a risk assessment for bull trout introduction into Sullivan Lake, northeastern, Washington including population donor sources and resident species Information tables associated with a risk assessment for bull trout introduction into Sullivan Lake, northeastern, Washington including population donor sources and resident species
Introduction and translocation programs require thoughtful planning to increase the likelihood of success and to understand the benefits, risks, and constraints of such programs. A risk assessment was completed for bull trout introduction into the Sullivan Lake and Harvey Creek watershed, northeastern Washington. The risk assessment was designed to evaluate potential risks to resident...
Spatial and temporal survey of waterborne myxozoan parasites in the Lake Sammamish watershed, Washington, from 2019 - 2021 Spatial and temporal survey of waterborne myxozoan parasites in the Lake Sammamish watershed, Washington, from 2019 - 2021
There is a fundamental knowledge gap on the distribution, prevalence, intensity, and ecology of salmonid myxozoan parasites in the Lake Sammamish watershed, Washington. To address this knowledge gap, we tested water samples for Ceratonova shasta, Parvicapsula minibicornis and Tetracapsuloides bryosalmonae DNA from 84 sites distributed throughout the Lake Sammamish watershed in fall 2019...
Filter Total Items: 41
Development of a quantitative PCR assay for detecting northwest salamander (ambystoma gracile) in environmental DNA samples Development of a quantitative PCR assay for detecting northwest salamander (ambystoma gracile) in environmental DNA samples
We developed a primer and probe based quantitative PCR assay for use with environmental DNA to detect Northwest salamander (Ambystoma gracile), a species endemic to the temperate Pacific coastal region of North America. The assay targets a region in the mitochondrial DNA (mtDNA) D-loop gene. Tests of the assay were performed in silico (using the NCBI BLAST tool), in vitro (using DNA...
Authors
Marshal S. Hoy, Carl O. Ostberg
Risk assessment for bull trout introduction into Sullivan Lake and Harvey Creek, northeastern Washington Risk assessment for bull trout introduction into Sullivan Lake and Harvey Creek, northeastern Washington
The Kalispel Tribe of Indians (KT), U. S. Fish and Wildlife Service, and Washington Department of Fish and Wildlife are engaged in conservation of bull trout (Salvelinus confluentus) in the Lake Pend Oreille (LPO) Core Area. The LPO is a complex habitat core area which falls within three states (Montana, Idaho, and Washington) and a tribal entity. As part of the conservation process, KT...
Authors
Jill M. Hardiman, Rachel B. Breyta, Carl O. Ostberg
Growth, survival, and cohort formation of juvenile Lost River (Deltistes luxatus) and shortnose suckers (Chasmistes brevirostris) in Upper Klamath Lake, Oregon, and Clear Lake Reservoir, California—2019 Monitoring Report Growth, survival, and cohort formation of juvenile Lost River (Deltistes luxatus) and shortnose suckers (Chasmistes brevirostris) in Upper Klamath Lake, Oregon, and Clear Lake Reservoir, California—2019 Monitoring Report
Populations of federally endangered Lost River (Deltistes luxatus) and shortnose suckers (Chasmistes brevirostris) in Upper Klamath Lake, Oregon, and Clear Lake Reservoir (hereinafter, Clear Lake), California, are experiencing long-term decreases in abundance. Upper Klamath Lake populations are decreasing not only due to adult mortality, which is relatively low, but also because they are...
Authors
Ryan J. Bart, Caylen M. Kelsey, Summer M. Burdick, Marshal S. Hoy, Carl O. Ostberg
Ontogeny of eDNA shedding during early development in Chinook Salmon (Oncorhynchus tshawytscha) Ontogeny of eDNA shedding during early development in Chinook Salmon (Oncorhynchus tshawytscha)
Knowledge of the timing of major life history events in aquatic species is important for informing conservation and resource management planning. Accordingly, surveys of environmental DNA (eDNA) have been performed to determine the efficacy of eDNA for providing information on life history events, primarily focusing on the timing of events associated with spawning, and these studies have...
Authors
Carl O. Ostberg, Dorothy M. Chase
Survival and growth of suckers in mesocosms at three locations within Upper Klamath Lake, Oregon, 2018 Survival and growth of suckers in mesocosms at three locations within Upper Klamath Lake, Oregon, 2018
Executive Summary Due to high mortality in the first year or two of life, Lost River (Deltistes luxatus sp.) and Shortnose suckers (Chasmistes brevirostris sp.) in Upper Klamath Lake, Oregon rarely reach maturity. In 2015, the U.S. Fish and Wildlife Service began the Sucker Assisted Rearing Program (SARP) to improve early life survival before releasing the fish back into Upper Klamath...
Authors
Summer M. Burdick, Carla M. Conway, Carl O. Ostberg, Ryan J. Bart, Diane G. Elliott
Environmental DNA is an effective tool to track recolonizing migratory fish following large‐scale dam removal Environmental DNA is an effective tool to track recolonizing migratory fish following large‐scale dam removal
Environmental DNA (eDNA) has emerged as a potentially powerful tool for use in conservation and resource management, including for tracking the recolonization dynamics of fish populations. We used eDNA to assess the effectiveness of dam removal to restore fish passage on the Elwha River in Washington State (USA). Using a suite of 11 species‐specific eDNA polymerase chain reaction (PCR)...
Authors
Jeffrey J. Duda, Marshal S. Hoy, Dorothy M. Chase, George R. Pess, Samuel J. Brenkman, Michael M McHenry, Carl O. Ostberg
Non-USGS Publications**
Ristow, S.S., L.D. Grabowski, C.O. Ostberg, B. Robison, and G.H. Thorgaard. 1998. Development of long-term cell lines from homozygous clones of rainbow trout. Journal of Aquatic Animal Health 10(1): 75-82. DOI: 10.1577/1548-8667(1998)010<0075:DOLTCL>2.0.CO;2.
Peek, A.S., P.A. Wheeler, C.O. Ostberg, and G.H. Thorgaard. 1997. A minichromosome carrying a pigmentation gene and brook trout DNA sequences in transgenic rainbow trout. Genome 40(5): 594-599. DOI: 10.1139/g97-778.
**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
*Disclaimer: Listing outside positions with professional scientific organizations on this Staff Profile are for informational purposes only and do not constitute an endorsement of those professional scientific organizations or their activities by the USGS, Department of the Interior, or U.S. Government