Joel G Putnam (Former Employee)
Science and Products
Screening toxic compounds using cellular assays
This study is designed to be a high-throughput alternative to traditional methods of using whole fish assays to screen compounds for toxicity. This approach involves an initial pre-screening of a chemical databank to select molecules possessing characteristics identified as predictive criteria for potential toxicity to the fathead minnow, followed by cytotoxicity screening of selected compounds...
Determination of the Acute Toxicity of Model-based Candidate Chemical Toxicants to Native and Nonnative Fish Species in Static Exposures
Invasive fishes are of considerable concern to aquatic resource managers. For example, the common carp (Cyprinus carpio), originally brought over from Eurasia sometime in the 1800s are now ubiquitous in U.S. waters. Although they have been around for over 100 years and have become part of U.S. culture, they are still highly detrimental to aquatic ecosystems and a method to eradicate and control of...
Data release for a novel tool to selectively deliver a control agent to filter-feeding silver and bighead carp Data release for a novel tool to selectively deliver a control agent to filter-feeding silver and bighead carp
Invasive carp pose substantial economic and ecological damage when populations are widespread in freshwater systems within the United States of America (USA). Resource managers in the USA have a limited number of chemical control tools to selectively remove nuisance fish. The present study examined whether Antimycin-A (antimycin) wax encapsulated microparticles could cause selective...
Data release for toxicity of Antimycin A incorporated management bait for grass carp Data release for toxicity of Antimycin A incorporated management bait for grass carp
The goal of this study was to develop and examine whether a management bait that can be used for selective control of grass carp. Our objectives were to 1) quantify the water-based 24-h LC50 of Antimycin-A for grass carp and rainbow trout, 2) quantify the 96-h LD50 of orally administered Antimycin-A laden bait for grass carp and rainbow trout, 3) quantify the leaching rate of Antimycin-A...
Metabolomic analysis of pheasantshell mussel (Ortmanniana pectorosa; Order Unionida) from a mass mortality event in the Clinch River, Virginia and Tennessee, USA Metabolomic analysis of pheasantshell mussel (Ortmanniana pectorosa; Order Unionida) from a mass mortality event in the Clinch River, Virginia and Tennessee, USA
This dataset uses the ITIS recommended Ortmanniana pectorosa, Conrad, 1834 in place of Actinonaias pectorosa, which was used in the Richard et al. 2020 manuscript. Biologists monitoring freshwater mussel (Order Unionida) populations rely on behavioral, often subjective, symptoms to identify “sick” or stressed animals, such as gaping valves and slow response to probing and lack clinical...
Indiana mussel metabolomics data analysis Indiana mussel metabolomics data analysis
This repository contains code to cluster a sample of Mussels based up their metabolic levels via Non-Metric Multidimensional Scaling (NMDS) and distance based-Redundancy Analysis (db-RDA). Mussel sample is broken down and examined by catch location, sex, and species. The code for this project assumes the reader is familiar with R and clustering techniques.
Characterization of occurrence and abundance of targeted metabolites in unionid mussels from three streams Characterization of occurrence and abundance of targeted metabolites in unionid mussels from three streams
The data consists of targeted metabolite analysis (using quadrupole time-of-flight mass spectroscopy;UHPLC-QTOF-MS) of hemolymph from two freshwater mussel species (Lampsilis cardium and L. siliquoidea) from three streams in Indiana. Concentrations of metabolites were modeled by species, stream and sex to determine the influence of each parameter.
A Novel Gonadotropic Microsporidian Parasite (Microsporidium clinchi n. sp.) Infecting a Declining Population of Pheasantshell Mussels (Actinonaias pectorosa) (Unioinidae) from the Clinch River, USA A Novel Gonadotropic Microsporidian Parasite (Microsporidium clinchi n. sp.) Infecting a Declining Population of Pheasantshell Mussels (Actinonaias pectorosa) (Unioinidae) from the Clinch River, USA
Freshwater mussels of the order Unionida are among the most endangered animal groups globally, but the causes of population declines are often enigmatic with little known about the role of disease. In 2018, we collected wild adult pheasantshell (Actinonaias pectorosa) and mucket (Actinonaias ligamentina) during an epidemiologic survey investigating an ongoing mussel mass mortality event...
Filter Total Items: 13
A novel tool to selectively deliver a control agent to filter-feeding silver and bighead carp A novel tool to selectively deliver a control agent to filter-feeding silver and bighead carp
Invasive carp pose substantial economic and ecological damage when populations are widespread in freshwater systems within the United States. Resource managers in the United States have few chemical control tools to selectively remove nuisance fish. This study examined whether Antimycin–A (antimycin) wax encapsulated microparticles could cause selective lethality in invasive carps. The...
Authors
Blake W. Sauey, Gavin N. Saari, Joel G. Putnam, Justine E. Nelson, James J. Wamboldt, J. Nolan Steiner, Robin D. Calfee
Toxicity of a management bait for grass carp (Ctenopharyngodon idella) incorporated with Antimycin A Toxicity of a management bait for grass carp (Ctenopharyngodon idella) incorporated with Antimycin A
No current technology can specifically target grass carp (Ctenopharyngodon idella) for control within aquatic ecosystems. Rotenone and Carbon Dioxide-Carp are currently the only available registered pesticides for grass carp; they are nonselective and typically applied throughout the water, equally exposing target and native species. A more selective control tool or pesticide application...
Authors
James J. Wamboldt, J. Nolan Steiner, Blake W. Sauey, Bryan Lada, Joel G. Putnam, Brianne Marjorie Korducki, Gavin N. Saari
Mussel mass mortality in the Clinch River, USA: Metabolomics detects affected pathways and biomarkers of stress Mussel mass mortality in the Clinch River, USA: Metabolomics detects affected pathways and biomarkers of stress
Biologists monitoring freshwater mussel (order Unionida) populations rely on behavioral, often subjective, signs to identify moribund (“sick”) or stressed mussels, such as gaping valves and slow response to probing, and they lack clinical indicators to support a diagnosis. As part of a multi-year study to investigate causes of reoccurring mortality of pheasantshell (Ortmanniana pectorosa...
Authors
Joel G. Putnam, J. Nolan Steiner, Jordon Richard, Eric Leis, Tony Goldberg, Christopher D. Dunn, Rose Agbalog, Susan Knowles, Diane L. Waller
Targeted metabolomics characterizes metabolite occurrence and variability in stable freshwater mussel populations Targeted metabolomics characterizes metabolite occurrence and variability in stable freshwater mussel populations
Freshwater mussels (order Unionida) play a key role in freshwater systems as ecosystem engineers and indicators of aquatic ecosystem health. The fauna is globally imperilled due to a diversity of suspected factors; however, causes for many population declines and mortality events remain unconfirmed due partly to limited health assessment tools. Mussel-monitoring activities often rely on...
Authors
Diane L. Waller, Joel Putnam, J. Nolan Steiner, Brant Fisher, Grant N. Burcham, John W. Oliver, Stephen B. Smith, Richard A. Erickson, Anne Remek, Nancy Bodeker
Further bacteriological analysis of annual Pheasantshell (Actinonaias pectorosa) mussel mortality events in the Clinch River (Virginia/Tennessee), USA, reveals a consistent association with Yokenella Regensburgei Further bacteriological analysis of annual Pheasantshell (Actinonaias pectorosa) mussel mortality events in the Clinch River (Virginia/Tennessee), USA, reveals a consistent association with Yokenella Regensburgei
Pheasantshell (Actinonaias pectorosa) mussels in the Clinch River (Tennessee/Virginia, USA) have declined dramatically in recent years. The bacterium Yokenella regensburgei was first isolated with high prevalence from Pheasantshells during the peak of a 2017 mortality event, but it was not identified after mortality subsided a few months later. Since 2017, Pheasantshell mortality in the...
Authors
Eric Leis, Sara Dziki, Jordan Richard, Rose Agbalog, Diane L. Waller, Joel G. Putnam, Susan Knowles, Tony Goldberg
Freshwater mussels show elevated viral richness and intensity during a mortality event Freshwater mussels show elevated viral richness and intensity during a mortality event
Freshwater mussels (Unionida) are among the world’s most imperiled taxa, but the relationship between freshwater mussel mortality events and infectious disease is largely unstudied. We surveyed viromes of a widespread and abundant species (mucket, Actinonaias ligamentina; syn: Ortmanniana ligamentina) experiencing a mortality event of unknown etiology in the Huron River, Michigan, in...
Authors
Jordan Richard, Eric Leis, Christopher D. Dunn, Cleyo Harris, Rose Agbalog, Lewis J. Campbell, Susan Knowles, Diane L. Waller, Joel G. Putnam, Tony Goldberg
Indiana mussel metabolomics data analysis Indiana mussel metabolomics data analysis
This repository contains code to cluster a sample of Mussels based up their metabolic levels via Non-Metric Multidimensional Scaling (NMDS) and distance based-Redundancy Analysis (db-RDA). Mussel sample is broken down and examined by catch location, sex, and species. The code for this project assumes the reader is familiar with R and clustering techniques.
Science and Products
Screening toxic compounds using cellular assays
This study is designed to be a high-throughput alternative to traditional methods of using whole fish assays to screen compounds for toxicity. This approach involves an initial pre-screening of a chemical databank to select molecules possessing characteristics identified as predictive criteria for potential toxicity to the fathead minnow, followed by cytotoxicity screening of selected compounds...
Determination of the Acute Toxicity of Model-based Candidate Chemical Toxicants to Native and Nonnative Fish Species in Static Exposures
Invasive fishes are of considerable concern to aquatic resource managers. For example, the common carp (Cyprinus carpio), originally brought over from Eurasia sometime in the 1800s are now ubiquitous in U.S. waters. Although they have been around for over 100 years and have become part of U.S. culture, they are still highly detrimental to aquatic ecosystems and a method to eradicate and control of...
Data release for a novel tool to selectively deliver a control agent to filter-feeding silver and bighead carp Data release for a novel tool to selectively deliver a control agent to filter-feeding silver and bighead carp
Invasive carp pose substantial economic and ecological damage when populations are widespread in freshwater systems within the United States of America (USA). Resource managers in the USA have a limited number of chemical control tools to selectively remove nuisance fish. The present study examined whether Antimycin-A (antimycin) wax encapsulated microparticles could cause selective...
Data release for toxicity of Antimycin A incorporated management bait for grass carp Data release for toxicity of Antimycin A incorporated management bait for grass carp
The goal of this study was to develop and examine whether a management bait that can be used for selective control of grass carp. Our objectives were to 1) quantify the water-based 24-h LC50 of Antimycin-A for grass carp and rainbow trout, 2) quantify the 96-h LD50 of orally administered Antimycin-A laden bait for grass carp and rainbow trout, 3) quantify the leaching rate of Antimycin-A...
Metabolomic analysis of pheasantshell mussel (Ortmanniana pectorosa; Order Unionida) from a mass mortality event in the Clinch River, Virginia and Tennessee, USA Metabolomic analysis of pheasantshell mussel (Ortmanniana pectorosa; Order Unionida) from a mass mortality event in the Clinch River, Virginia and Tennessee, USA
This dataset uses the ITIS recommended Ortmanniana pectorosa, Conrad, 1834 in place of Actinonaias pectorosa, which was used in the Richard et al. 2020 manuscript. Biologists monitoring freshwater mussel (Order Unionida) populations rely on behavioral, often subjective, symptoms to identify “sick” or stressed animals, such as gaping valves and slow response to probing and lack clinical...
Indiana mussel metabolomics data analysis Indiana mussel metabolomics data analysis
This repository contains code to cluster a sample of Mussels based up their metabolic levels via Non-Metric Multidimensional Scaling (NMDS) and distance based-Redundancy Analysis (db-RDA). Mussel sample is broken down and examined by catch location, sex, and species. The code for this project assumes the reader is familiar with R and clustering techniques.
Characterization of occurrence and abundance of targeted metabolites in unionid mussels from three streams Characterization of occurrence and abundance of targeted metabolites in unionid mussels from three streams
The data consists of targeted metabolite analysis (using quadrupole time-of-flight mass spectroscopy;UHPLC-QTOF-MS) of hemolymph from two freshwater mussel species (Lampsilis cardium and L. siliquoidea) from three streams in Indiana. Concentrations of metabolites were modeled by species, stream and sex to determine the influence of each parameter.
A Novel Gonadotropic Microsporidian Parasite (Microsporidium clinchi n. sp.) Infecting a Declining Population of Pheasantshell Mussels (Actinonaias pectorosa) (Unioinidae) from the Clinch River, USA A Novel Gonadotropic Microsporidian Parasite (Microsporidium clinchi n. sp.) Infecting a Declining Population of Pheasantshell Mussels (Actinonaias pectorosa) (Unioinidae) from the Clinch River, USA
Freshwater mussels of the order Unionida are among the most endangered animal groups globally, but the causes of population declines are often enigmatic with little known about the role of disease. In 2018, we collected wild adult pheasantshell (Actinonaias pectorosa) and mucket (Actinonaias ligamentina) during an epidemiologic survey investigating an ongoing mussel mass mortality event...
Filter Total Items: 13
A novel tool to selectively deliver a control agent to filter-feeding silver and bighead carp A novel tool to selectively deliver a control agent to filter-feeding silver and bighead carp
Invasive carp pose substantial economic and ecological damage when populations are widespread in freshwater systems within the United States. Resource managers in the United States have few chemical control tools to selectively remove nuisance fish. This study examined whether Antimycin–A (antimycin) wax encapsulated microparticles could cause selective lethality in invasive carps. The...
Authors
Blake W. Sauey, Gavin N. Saari, Joel G. Putnam, Justine E. Nelson, James J. Wamboldt, J. Nolan Steiner, Robin D. Calfee
Toxicity of a management bait for grass carp (Ctenopharyngodon idella) incorporated with Antimycin A Toxicity of a management bait for grass carp (Ctenopharyngodon idella) incorporated with Antimycin A
No current technology can specifically target grass carp (Ctenopharyngodon idella) for control within aquatic ecosystems. Rotenone and Carbon Dioxide-Carp are currently the only available registered pesticides for grass carp; they are nonselective and typically applied throughout the water, equally exposing target and native species. A more selective control tool or pesticide application...
Authors
James J. Wamboldt, J. Nolan Steiner, Blake W. Sauey, Bryan Lada, Joel G. Putnam, Brianne Marjorie Korducki, Gavin N. Saari
Mussel mass mortality in the Clinch River, USA: Metabolomics detects affected pathways and biomarkers of stress Mussel mass mortality in the Clinch River, USA: Metabolomics detects affected pathways and biomarkers of stress
Biologists monitoring freshwater mussel (order Unionida) populations rely on behavioral, often subjective, signs to identify moribund (“sick”) or stressed mussels, such as gaping valves and slow response to probing, and they lack clinical indicators to support a diagnosis. As part of a multi-year study to investigate causes of reoccurring mortality of pheasantshell (Ortmanniana pectorosa...
Authors
Joel G. Putnam, J. Nolan Steiner, Jordon Richard, Eric Leis, Tony Goldberg, Christopher D. Dunn, Rose Agbalog, Susan Knowles, Diane L. Waller
Targeted metabolomics characterizes metabolite occurrence and variability in stable freshwater mussel populations Targeted metabolomics characterizes metabolite occurrence and variability in stable freshwater mussel populations
Freshwater mussels (order Unionida) play a key role in freshwater systems as ecosystem engineers and indicators of aquatic ecosystem health. The fauna is globally imperilled due to a diversity of suspected factors; however, causes for many population declines and mortality events remain unconfirmed due partly to limited health assessment tools. Mussel-monitoring activities often rely on...
Authors
Diane L. Waller, Joel Putnam, J. Nolan Steiner, Brant Fisher, Grant N. Burcham, John W. Oliver, Stephen B. Smith, Richard A. Erickson, Anne Remek, Nancy Bodeker
Further bacteriological analysis of annual Pheasantshell (Actinonaias pectorosa) mussel mortality events in the Clinch River (Virginia/Tennessee), USA, reveals a consistent association with Yokenella Regensburgei Further bacteriological analysis of annual Pheasantshell (Actinonaias pectorosa) mussel mortality events in the Clinch River (Virginia/Tennessee), USA, reveals a consistent association with Yokenella Regensburgei
Pheasantshell (Actinonaias pectorosa) mussels in the Clinch River (Tennessee/Virginia, USA) have declined dramatically in recent years. The bacterium Yokenella regensburgei was first isolated with high prevalence from Pheasantshells during the peak of a 2017 mortality event, but it was not identified after mortality subsided a few months later. Since 2017, Pheasantshell mortality in the...
Authors
Eric Leis, Sara Dziki, Jordan Richard, Rose Agbalog, Diane L. Waller, Joel G. Putnam, Susan Knowles, Tony Goldberg
Freshwater mussels show elevated viral richness and intensity during a mortality event Freshwater mussels show elevated viral richness and intensity during a mortality event
Freshwater mussels (Unionida) are among the world’s most imperiled taxa, but the relationship between freshwater mussel mortality events and infectious disease is largely unstudied. We surveyed viromes of a widespread and abundant species (mucket, Actinonaias ligamentina; syn: Ortmanniana ligamentina) experiencing a mortality event of unknown etiology in the Huron River, Michigan, in...
Authors
Jordan Richard, Eric Leis, Christopher D. Dunn, Cleyo Harris, Rose Agbalog, Lewis J. Campbell, Susan Knowles, Diane L. Waller, Joel G. Putnam, Tony Goldberg
Indiana mussel metabolomics data analysis Indiana mussel metabolomics data analysis
This repository contains code to cluster a sample of Mussels based up their metabolic levels via Non-Metric Multidimensional Scaling (NMDS) and distance based-Redundancy Analysis (db-RDA). Mussel sample is broken down and examined by catch location, sex, and species. The code for this project assumes the reader is familiar with R and clustering techniques.