Maureen K Purcell, Ph.D. (Former Employee)
Science and Products
Filter Total Items: 14
Filter Total Items: 88
Resistance and Protective Immunity in Redfish Lake Sockeye Salmon Exposed to M Type Infectious Hematopoietic Necrosis Virus (IHNV) Resistance and Protective Immunity in Redfish Lake Sockeye Salmon Exposed to M Type Infectious Hematopoietic Necrosis Virus (IHNV)
Differential virulence of infectious hematopoietic necrosis virus (IHNV) isolates from the U and M phylogenetic subgroups is clearly evident in the Redfish Lake (RFL) strain of sockeye salmon Oncorhynchus nerka. In these fish, experimental immersion challenges with U isolates cause extremely high mortality and M isolates cause low or no mortality. When survivors of M virus immersion...
Authors
Gael Kurath, Kyle Garver, Maureen K. Purcell, Scott E. LaPatra
Infectious haematopoietic necrosis virus genogroup-specific virulence mechanisms in sockeye salmon, Oncorhynchus nerka (Walbaum), from Redfish Lake, Idaho Infectious haematopoietic necrosis virus genogroup-specific virulence mechanisms in sockeye salmon, Oncorhynchus nerka (Walbaum), from Redfish Lake, Idaho
Characterization of infectious haematopoietic necrosis virus (IHNV) field isolates from North America has established three main genogroups (U, M and L) that differ in host-specific virulence. In sockeye salmon, Oncorhynchus nerka, the U genogroup is highly virulent, whereas the M genogroup is nearly non-pathogenic. In this study, we sought to characterize the virus-host dynamics that...
Authors
M. K. Purcell, K.A. Garver, C. Conway, D.G. Elliott, Gael Kurath
Development and validation of a quantitative PCR to detect Parvicapsula minibicornis and comparison to histologically ranked infection of juvenile Chinook salmon, Oncorhynchus tshawytscha (Walbaum), from the Klamath River, USA Development and validation of a quantitative PCR to detect Parvicapsula minibicornis and comparison to histologically ranked infection of juvenile Chinook salmon, Oncorhynchus tshawytscha (Walbaum), from the Klamath River, USA
Parvicapsula minibicornis is a myxosporean parasite that is associated with disease in Pacific salmon during their freshwater life history phase. This study reports the development of a quantitative (real-time) polymerase chain reaction (QPCR) to detect P. minibicornis DNA. The QPCR assay targets the 18S ribosomal subunit gene. A plasmid DNA control was developed to calibrate cycle...
Authors
K. TRUE, M. K. Purcell, J.S. Foott
Differential virulence mechanisms of infectious hematopoietic necrosis virus in rainbow trout (Oncorhynchus mykiss) include host entry and virus replication kinetics Differential virulence mechanisms of infectious hematopoietic necrosis virus in rainbow trout (Oncorhynchus mykiss) include host entry and virus replication kinetics
Host specificity is a phenomenon exhibited by all viruses. For the fish rhabdovirus infectious hematopoietic necrosis virus (IHNV), differential specificity of virus strains from the U and M genogroups has been established both in the field and in experimental challenges. In rainbow trout (Oncorhynchus mykiss), M IHNV strains are consistently more prevalent and more virulent than U IHNV...
Authors
M.M.D. Penaranda, M. K. Purcell, Gael Kurath
Characterization of the interferon genes in homozygous rainbow trout reveals two novel genes, alternate splicing and differential regulation of duplicated genes Characterization of the interferon genes in homozygous rainbow trout reveals two novel genes, alternate splicing and differential regulation of duplicated genes
The genes encoding the type I and type II interferons (IFNs) have previously been identified in rainbow trout and their proteins partially characterized. These previous studies reported a single type II IFN (rtIFN-??) and three rainbow trout type I IFN genes that are classified into either group I (rtIFN1, rtIFN2) or group II (rtIFN3). In this present study, we report the identification...
Authors
M. K. Purcell, K.J. Laing, J.C. Woodson, G.H. Thorgaard, J.D. Hansen
Differential survival of Ichthyophonus isolates indicates parasite adaptation to its host environment Differential survival of Ichthyophonus isolates indicates parasite adaptation to its host environment
In vitro viability of Ichthyophonus spp. spores in seawater and freshwater corresponded with the water type of the host from which the spores were isolated. Among Ichthyophonus spp. spores from both marine and freshwater fish hosts (Pacific herring, Clupea pallasii, and rainbow trout, Oncorhynchus mykiss, respectively), viability was significantly greater (P 0.05) after incubation in...
Authors
P.K. Hershberger, C.A. Pacheco, J.L. Gregg, M. K. Purcell, S. E. LaPatra
Non-USGS Publications**
Purcell, M., G. Mackey, E. LaHood, H. Huber, and L. Park. 2004. Molecular methods for the genetic identification of salmonid prey from Pacific harbor seal (Phoca vitulina richardsi ) scat. Fish. Bull. 1002: 213-220.
Lage, C., M. Purcell, M. Forgarty, and I. Kornfield. 2001. Microsatellite evaluation of haddock (Melanogrammus aeglefinus) stocks in the northwest Atlantic Ocean. Can. J. Fish. Aquat. Sci. 58: 982-990. DOI: 10.1139/cjfas-58-5-982.
Purcell, M.K., J-L. Mu, D.C. Higgins, R. Elango, H. Whitmore, S. Harris, and B. Paigen. 2001. Fine mapping of Ath6, a quantitative trait locus for atherosclerosis in mice. Mammalian Genome 12(7): 495-500. DOI: 10.1007/s00335001-0006-9.
Purcell, M.K., I.L. Kornfield, M.J. Fogarty and A. Parker. (1996) Interdecadal heterogeneity of mitochondrial DNA in Atlantic haddock (Melanogrammus aeglefinus) from Georges Bank. Molecular Marine Biology and Biotechnology. 5:185-192.
**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
Science and Products
Filter Total Items: 14
Filter Total Items: 88
Resistance and Protective Immunity in Redfish Lake Sockeye Salmon Exposed to M Type Infectious Hematopoietic Necrosis Virus (IHNV) Resistance and Protective Immunity in Redfish Lake Sockeye Salmon Exposed to M Type Infectious Hematopoietic Necrosis Virus (IHNV)
Differential virulence of infectious hematopoietic necrosis virus (IHNV) isolates from the U and M phylogenetic subgroups is clearly evident in the Redfish Lake (RFL) strain of sockeye salmon Oncorhynchus nerka. In these fish, experimental immersion challenges with U isolates cause extremely high mortality and M isolates cause low or no mortality. When survivors of M virus immersion...
Authors
Gael Kurath, Kyle Garver, Maureen K. Purcell, Scott E. LaPatra
Infectious haematopoietic necrosis virus genogroup-specific virulence mechanisms in sockeye salmon, Oncorhynchus nerka (Walbaum), from Redfish Lake, Idaho Infectious haematopoietic necrosis virus genogroup-specific virulence mechanisms in sockeye salmon, Oncorhynchus nerka (Walbaum), from Redfish Lake, Idaho
Characterization of infectious haematopoietic necrosis virus (IHNV) field isolates from North America has established three main genogroups (U, M and L) that differ in host-specific virulence. In sockeye salmon, Oncorhynchus nerka, the U genogroup is highly virulent, whereas the M genogroup is nearly non-pathogenic. In this study, we sought to characterize the virus-host dynamics that...
Authors
M. K. Purcell, K.A. Garver, C. Conway, D.G. Elliott, Gael Kurath
Development and validation of a quantitative PCR to detect Parvicapsula minibicornis and comparison to histologically ranked infection of juvenile Chinook salmon, Oncorhynchus tshawytscha (Walbaum), from the Klamath River, USA Development and validation of a quantitative PCR to detect Parvicapsula minibicornis and comparison to histologically ranked infection of juvenile Chinook salmon, Oncorhynchus tshawytscha (Walbaum), from the Klamath River, USA
Parvicapsula minibicornis is a myxosporean parasite that is associated with disease in Pacific salmon during their freshwater life history phase. This study reports the development of a quantitative (real-time) polymerase chain reaction (QPCR) to detect P. minibicornis DNA. The QPCR assay targets the 18S ribosomal subunit gene. A plasmid DNA control was developed to calibrate cycle...
Authors
K. TRUE, M. K. Purcell, J.S. Foott
Differential virulence mechanisms of infectious hematopoietic necrosis virus in rainbow trout (Oncorhynchus mykiss) include host entry and virus replication kinetics Differential virulence mechanisms of infectious hematopoietic necrosis virus in rainbow trout (Oncorhynchus mykiss) include host entry and virus replication kinetics
Host specificity is a phenomenon exhibited by all viruses. For the fish rhabdovirus infectious hematopoietic necrosis virus (IHNV), differential specificity of virus strains from the U and M genogroups has been established both in the field and in experimental challenges. In rainbow trout (Oncorhynchus mykiss), M IHNV strains are consistently more prevalent and more virulent than U IHNV...
Authors
M.M.D. Penaranda, M. K. Purcell, Gael Kurath
Characterization of the interferon genes in homozygous rainbow trout reveals two novel genes, alternate splicing and differential regulation of duplicated genes Characterization of the interferon genes in homozygous rainbow trout reveals two novel genes, alternate splicing and differential regulation of duplicated genes
The genes encoding the type I and type II interferons (IFNs) have previously been identified in rainbow trout and their proteins partially characterized. These previous studies reported a single type II IFN (rtIFN-??) and three rainbow trout type I IFN genes that are classified into either group I (rtIFN1, rtIFN2) or group II (rtIFN3). In this present study, we report the identification...
Authors
M. K. Purcell, K.J. Laing, J.C. Woodson, G.H. Thorgaard, J.D. Hansen
Differential survival of Ichthyophonus isolates indicates parasite adaptation to its host environment Differential survival of Ichthyophonus isolates indicates parasite adaptation to its host environment
In vitro viability of Ichthyophonus spp. spores in seawater and freshwater corresponded with the water type of the host from which the spores were isolated. Among Ichthyophonus spp. spores from both marine and freshwater fish hosts (Pacific herring, Clupea pallasii, and rainbow trout, Oncorhynchus mykiss, respectively), viability was significantly greater (P 0.05) after incubation in...
Authors
P.K. Hershberger, C.A. Pacheco, J.L. Gregg, M. K. Purcell, S. E. LaPatra
Non-USGS Publications**
Purcell, M., G. Mackey, E. LaHood, H. Huber, and L. Park. 2004. Molecular methods for the genetic identification of salmonid prey from Pacific harbor seal (Phoca vitulina richardsi ) scat. Fish. Bull. 1002: 213-220.
Lage, C., M. Purcell, M. Forgarty, and I. Kornfield. 2001. Microsatellite evaluation of haddock (Melanogrammus aeglefinus) stocks in the northwest Atlantic Ocean. Can. J. Fish. Aquat. Sci. 58: 982-990. DOI: 10.1139/cjfas-58-5-982.
Purcell, M.K., J-L. Mu, D.C. Higgins, R. Elango, H. Whitmore, S. Harris, and B. Paigen. 2001. Fine mapping of Ath6, a quantitative trait locus for atherosclerosis in mice. Mammalian Genome 12(7): 495-500. DOI: 10.1007/s00335001-0006-9.
Purcell, M.K., I.L. Kornfield, M.J. Fogarty and A. Parker. (1996) Interdecadal heterogeneity of mitochondrial DNA in Atlantic haddock (Melanogrammus aeglefinus) from Georges Bank. Molecular Marine Biology and Biotechnology. 5:185-192.
**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
*Disclaimer: Listing outside positions with professional scientific organizations on this Staff Profile are for informational purposes only and do not constitute an endorsement of those professional scientific organizations or their activities by the USGS, Department of the Interior, or U.S. Government