Maureen K Purcell, Ph.D. (Former Employee)
Science and Products
Filter Total Items: 14
Filter Total Items: 88
Analytical validation of a reverse transcriptase droplet digital PCR (RT-ddPCR) for quantitative detection of infectious hematopoietic necrosis virus Analytical validation of a reverse transcriptase droplet digital PCR (RT-ddPCR) for quantitative detection of infectious hematopoietic necrosis virus
Infectious hematopoietic necrosis virus (IHNV) is an important pathogen of salmonid fishes. A validated universal reverse transcriptase quantitative PCR (RT-qPCR) assay that can quantify levels of IHNV in fish tissues has been previously reported. In the present study, we adapted the published set of IHNV primers and probe for use in a reverse-transcriptase droplet digital PCR (RT-ddPCR)...
Authors
Peng Jia, Maureen K. Purcell, Guang Pan, Jinjin Wang, Shifu Kan, Yin Liu, Xiaocong Zheng, Xiujie SHi, Junqiang He, Li Yu, Qunyi Hua, Tikang Lu, Wensheng Lan, James Winton, Ningyi Jin, Hong Liu
Optimization of a Plaque Neutralization Test (PNT) to identify the exposure history of Pacific Herring to viral hemorrhagic septicemia virus (VHSV) Optimization of a Plaque Neutralization Test (PNT) to identify the exposure history of Pacific Herring to viral hemorrhagic septicemia virus (VHSV)
Methods for a plaque neutralization test (PNT) were optimized for the detection and quantification of viral hemorrhagic septicemia virus (VHSV) neutralizing activity in the plasma of Pacific Herring Clupea pallasii. The PNT was complement dependent, as neutralizing activity was attenuated by heat inactivation; further, neutralizing activity was mostly restored by the addition of...
Authors
Lucas Hart, Ashley MacKenzie, Maureen K. Purcell, Rachel L. Thompson, Paul Hershberger
Molecular characterization of a novel orthomyxovirus from rainbow and steelhead trout (Oncorhynchus mykiss) Molecular characterization of a novel orthomyxovirus from rainbow and steelhead trout (Oncorhynchus mykiss)
A novel virus, rainbow trout orthomyxovirus (RbtOV), was isolated in 1997 and again in 2000 from commercially-reared rainbow trout (Oncorhynchus mykiss) in Idaho, USA. The virus grew optimally in the CHSE-214 cell line at 15°C producing a diffuse cytopathic effect; however, juvenile rainbow trout exposed to cell culture-grown virus showed no mortality or gross pathology. Electron...
Authors
William N. Batts, Scott E. LaPatra, Ryan Katona, Eric Leis, Terry Fei Fan Ng, Marine S.O. Bruieuc, Rachel Breyta, Maureen K. Purcell, Thomas B. Waltzek, Eric Delwart, James Winton
Detecting Renibacterium salmoninarum in wild brown trout by use of multiple organ samples and diagnostic methods Detecting Renibacterium salmoninarum in wild brown trout by use of multiple organ samples and diagnostic methods
Renibacterium salmoninarum, the causative agent of salmonid bacterial kidney disease (BKD), is endemic in many wild trout species in northerly regions. The aim of the present study was to determine the optimal R. salmoninarum sampling/testing strategy for wild brown trout (Salmo trutta L.) populations in Iceland. Fish were netted in a lake and multiple organs—kidney, spleen, gills...
Authors
S. Guomundsdottir, Lynn M. Applegate, I.O. Arnason, A. Kristmundsson, Maureen K. Purcell, Diane G. Elliott
Inhibition of an aquatic rhabdovirus demonstrates promise of a broad-spectrum antiviral for use in aquaculture Inhibition of an aquatic rhabdovirus demonstrates promise of a broad-spectrum antiviral for use in aquaculture
Many enveloped viruses cause devastating disease in aquaculture, resulting in significant economic impact. LJ001 is a broad-spectrum antiviral compound that inhibits enveloped virus infections by specifically targeting phospholipids in the lipid bilayer via the production of singlet oxygen (1O2). This stabilizes positive curvature and decreases membrane fluidity, which inhibits virus...
Authors
Bethany F. Balmer, Rachel L. Powers, Ting-Hu Zhang, Jihye Lee, Frederic Vigant, Benhur Lee, Michael E. Jung, Maureen K. Purcell, Kevin Snekvik, Hector C. Aguilar
Herring Disease Program – Herring Disease Program II Herring Disease Program – Herring Disease Program II
No abstract available.
Authors
Paul Hershberger, Maureen K. Purcell
Non-USGS Publications**
Purcell, M., G. Mackey, E. LaHood, H. Huber, and L. Park. 2004. Molecular methods for the genetic identification of salmonid prey from Pacific harbor seal (Phoca vitulina richardsi ) scat. Fish. Bull. 1002: 213-220.
Lage, C., M. Purcell, M. Forgarty, and I. Kornfield. 2001. Microsatellite evaluation of haddock (Melanogrammus aeglefinus) stocks in the northwest Atlantic Ocean. Can. J. Fish. Aquat. Sci. 58: 982-990. DOI: 10.1139/cjfas-58-5-982.
Purcell, M.K., J-L. Mu, D.C. Higgins, R. Elango, H. Whitmore, S. Harris, and B. Paigen. 2001. Fine mapping of Ath6, a quantitative trait locus for atherosclerosis in mice. Mammalian Genome 12(7): 495-500. DOI: 10.1007/s00335001-0006-9.
Purcell, M.K., I.L. Kornfield, M.J. Fogarty and A. Parker. (1996) Interdecadal heterogeneity of mitochondrial DNA in Atlantic haddock (Melanogrammus aeglefinus) from Georges Bank. Molecular Marine Biology and Biotechnology. 5:185-192.
**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
Science and Products
Filter Total Items: 14
Filter Total Items: 88
Analytical validation of a reverse transcriptase droplet digital PCR (RT-ddPCR) for quantitative detection of infectious hematopoietic necrosis virus Analytical validation of a reverse transcriptase droplet digital PCR (RT-ddPCR) for quantitative detection of infectious hematopoietic necrosis virus
Infectious hematopoietic necrosis virus (IHNV) is an important pathogen of salmonid fishes. A validated universal reverse transcriptase quantitative PCR (RT-qPCR) assay that can quantify levels of IHNV in fish tissues has been previously reported. In the present study, we adapted the published set of IHNV primers and probe for use in a reverse-transcriptase droplet digital PCR (RT-ddPCR)...
Authors
Peng Jia, Maureen K. Purcell, Guang Pan, Jinjin Wang, Shifu Kan, Yin Liu, Xiaocong Zheng, Xiujie SHi, Junqiang He, Li Yu, Qunyi Hua, Tikang Lu, Wensheng Lan, James Winton, Ningyi Jin, Hong Liu
Optimization of a Plaque Neutralization Test (PNT) to identify the exposure history of Pacific Herring to viral hemorrhagic septicemia virus (VHSV) Optimization of a Plaque Neutralization Test (PNT) to identify the exposure history of Pacific Herring to viral hemorrhagic septicemia virus (VHSV)
Methods for a plaque neutralization test (PNT) were optimized for the detection and quantification of viral hemorrhagic septicemia virus (VHSV) neutralizing activity in the plasma of Pacific Herring Clupea pallasii. The PNT was complement dependent, as neutralizing activity was attenuated by heat inactivation; further, neutralizing activity was mostly restored by the addition of...
Authors
Lucas Hart, Ashley MacKenzie, Maureen K. Purcell, Rachel L. Thompson, Paul Hershberger
Molecular characterization of a novel orthomyxovirus from rainbow and steelhead trout (Oncorhynchus mykiss) Molecular characterization of a novel orthomyxovirus from rainbow and steelhead trout (Oncorhynchus mykiss)
A novel virus, rainbow trout orthomyxovirus (RbtOV), was isolated in 1997 and again in 2000 from commercially-reared rainbow trout (Oncorhynchus mykiss) in Idaho, USA. The virus grew optimally in the CHSE-214 cell line at 15°C producing a diffuse cytopathic effect; however, juvenile rainbow trout exposed to cell culture-grown virus showed no mortality or gross pathology. Electron...
Authors
William N. Batts, Scott E. LaPatra, Ryan Katona, Eric Leis, Terry Fei Fan Ng, Marine S.O. Bruieuc, Rachel Breyta, Maureen K. Purcell, Thomas B. Waltzek, Eric Delwart, James Winton
Detecting Renibacterium salmoninarum in wild brown trout by use of multiple organ samples and diagnostic methods Detecting Renibacterium salmoninarum in wild brown trout by use of multiple organ samples and diagnostic methods
Renibacterium salmoninarum, the causative agent of salmonid bacterial kidney disease (BKD), is endemic in many wild trout species in northerly regions. The aim of the present study was to determine the optimal R. salmoninarum sampling/testing strategy for wild brown trout (Salmo trutta L.) populations in Iceland. Fish were netted in a lake and multiple organs—kidney, spleen, gills...
Authors
S. Guomundsdottir, Lynn M. Applegate, I.O. Arnason, A. Kristmundsson, Maureen K. Purcell, Diane G. Elliott
Inhibition of an aquatic rhabdovirus demonstrates promise of a broad-spectrum antiviral for use in aquaculture Inhibition of an aquatic rhabdovirus demonstrates promise of a broad-spectrum antiviral for use in aquaculture
Many enveloped viruses cause devastating disease in aquaculture, resulting in significant economic impact. LJ001 is a broad-spectrum antiviral compound that inhibits enveloped virus infections by specifically targeting phospholipids in the lipid bilayer via the production of singlet oxygen (1O2). This stabilizes positive curvature and decreases membrane fluidity, which inhibits virus...
Authors
Bethany F. Balmer, Rachel L. Powers, Ting-Hu Zhang, Jihye Lee, Frederic Vigant, Benhur Lee, Michael E. Jung, Maureen K. Purcell, Kevin Snekvik, Hector C. Aguilar
Herring Disease Program – Herring Disease Program II Herring Disease Program – Herring Disease Program II
No abstract available.
Authors
Paul Hershberger, Maureen K. Purcell
Non-USGS Publications**
Purcell, M., G. Mackey, E. LaHood, H. Huber, and L. Park. 2004. Molecular methods for the genetic identification of salmonid prey from Pacific harbor seal (Phoca vitulina richardsi ) scat. Fish. Bull. 1002: 213-220.
Lage, C., M. Purcell, M. Forgarty, and I. Kornfield. 2001. Microsatellite evaluation of haddock (Melanogrammus aeglefinus) stocks in the northwest Atlantic Ocean. Can. J. Fish. Aquat. Sci. 58: 982-990. DOI: 10.1139/cjfas-58-5-982.
Purcell, M.K., J-L. Mu, D.C. Higgins, R. Elango, H. Whitmore, S. Harris, and B. Paigen. 2001. Fine mapping of Ath6, a quantitative trait locus for atherosclerosis in mice. Mammalian Genome 12(7): 495-500. DOI: 10.1007/s00335001-0006-9.
Purcell, M.K., I.L. Kornfield, M.J. Fogarty and A. Parker. (1996) Interdecadal heterogeneity of mitochondrial DNA in Atlantic haddock (Melanogrammus aeglefinus) from Georges Bank. Molecular Marine Biology and Biotechnology. 5:185-192.
**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
*Disclaimer: Listing outside positions with professional scientific organizations on this Staff Profile are for informational purposes only and do not constitute an endorsement of those professional scientific organizations or their activities by the USGS, Department of the Interior, or U.S. Government