Reynaldo Patino, PhD (Former Employee)
Science and Products
Filter Total Items: 64
Interaction of xenobiotics with estrogen receptors α and β and a putative plasma sex hormone-binding globulin from channel catfish (Ictalurus punctatus) Interaction of xenobiotics with estrogen receptors α and β and a putative plasma sex hormone-binding globulin from channel catfish (Ictalurus punctatus)
Estrogens are important regulators of physiological functions. Although environmental contaminants (xenoestrogens) which interfere with estrogen signaling are of increasing concern, there is only limited information about their ability to interact with estrogen-binding proteins (SHBG) or receptors (ER). Recombinant ER?? and ?? were obtained after transient transfection of COS-7 cells...
Authors
William L. Gale, Reynaldo Patino, Alec G. Maule
Phylogenetic sequence analysis, recombinant expression, and tissue distribution of a channel catfish estrogen receptor beta Phylogenetic sequence analysis, recombinant expression, and tissue distribution of a channel catfish estrogen receptor beta
An estrogen receptor β (ERβ) cDNA fragment was amplified by RT-PCR of total RNAextracted from liver and ovary of immature channel catfish. This cDNA fragment was used to screen an ovarian cDNA library made from an immature female fish. A clone was obtained that contained an open reading frame encoding a 575-amino-acid protein with a deduced molecular weight of 63.9 kDa. Maximum parsimony...
Authors
Zhenfang Xia, William L. Gale, Xiaotian Chang, David Langenau, Reynaldo Patino, Alec G. Maule, Llewellyn D. Densmore
Novel transcripts of the estrogen receptor α gene in channel catfish Novel transcripts of the estrogen receptor α gene in channel catfish
Complementary DNA libraries from liver and ovary of an immature female channel catfish were screened with a homologous ERα cDNA probe. The hepatic library yielded two new channel catfish ER cDNAs that encode N-terminal ERα variants of different sizes. Relative to the catfish ERα (medium size; 581 residues) previously reported, these new cDNAs encode Long-ERα (36 residues longer) and...
Authors
Reynaldo Patino, Zhenfang Xia, William L. Gale, Chunfa Wu, Alec G. Maule, Xiaotian Chang
Overview of a workshop on screening methods for detecting potential (anti-) estrogenic/androgenic chemicals in wildlife Overview of a workshop on screening methods for detecting potential (anti-) estrogenic/androgenic chemicals in wildlife
The U.S. Congress has passed legislation requiring the U.S. Environmental Protection Agency (U.S. EPA) to develop, validate, and implement screening tests for identifying potential endocrine-disrupting chemicals within 3 years. To aid in the identification of methods suitable for this purpose, the U.S. EPA, the Chemical Manufacturers Association, and the World Wildlife Fund sponsored...
Authors
Gerald T. Ankley, Ellen Mihaich, Ralph G. Stahl, Donald E. Tillitt, Theo Colborn, Suzzanne McMaster, Ron Miller, John Bantle, Pamela Campbell, Nancy Denslow, Richard L. Dickerson, Leroy C. Folmar, Michael Fry, John P. Giesy, L. Earl Gray, Patrick Guiney, Thomas Hutchinson, Sean W. Kennedy, Vincent Kramer, Gerald A. LeBlanc, Monte Mayes, Alison Nimrod, Reynaldo Patino, Richard Peterson, Richard Purdy, Robert Ringer, Peter C. Thomas, Les Touart, Glen Van Der Kraak, Tim Zacharewski
Science and Products
Filter Total Items: 64
Interaction of xenobiotics with estrogen receptors α and β and a putative plasma sex hormone-binding globulin from channel catfish (Ictalurus punctatus) Interaction of xenobiotics with estrogen receptors α and β and a putative plasma sex hormone-binding globulin from channel catfish (Ictalurus punctatus)
Estrogens are important regulators of physiological functions. Although environmental contaminants (xenoestrogens) which interfere with estrogen signaling are of increasing concern, there is only limited information about their ability to interact with estrogen-binding proteins (SHBG) or receptors (ER). Recombinant ER?? and ?? were obtained after transient transfection of COS-7 cells...
Authors
William L. Gale, Reynaldo Patino, Alec G. Maule
Phylogenetic sequence analysis, recombinant expression, and tissue distribution of a channel catfish estrogen receptor beta Phylogenetic sequence analysis, recombinant expression, and tissue distribution of a channel catfish estrogen receptor beta
An estrogen receptor β (ERβ) cDNA fragment was amplified by RT-PCR of total RNAextracted from liver and ovary of immature channel catfish. This cDNA fragment was used to screen an ovarian cDNA library made from an immature female fish. A clone was obtained that contained an open reading frame encoding a 575-amino-acid protein with a deduced molecular weight of 63.9 kDa. Maximum parsimony...
Authors
Zhenfang Xia, William L. Gale, Xiaotian Chang, David Langenau, Reynaldo Patino, Alec G. Maule, Llewellyn D. Densmore
Novel transcripts of the estrogen receptor α gene in channel catfish Novel transcripts of the estrogen receptor α gene in channel catfish
Complementary DNA libraries from liver and ovary of an immature female channel catfish were screened with a homologous ERα cDNA probe. The hepatic library yielded two new channel catfish ER cDNAs that encode N-terminal ERα variants of different sizes. Relative to the catfish ERα (medium size; 581 residues) previously reported, these new cDNAs encode Long-ERα (36 residues longer) and...
Authors
Reynaldo Patino, Zhenfang Xia, William L. Gale, Chunfa Wu, Alec G. Maule, Xiaotian Chang
Overview of a workshop on screening methods for detecting potential (anti-) estrogenic/androgenic chemicals in wildlife Overview of a workshop on screening methods for detecting potential (anti-) estrogenic/androgenic chemicals in wildlife
The U.S. Congress has passed legislation requiring the U.S. Environmental Protection Agency (U.S. EPA) to develop, validate, and implement screening tests for identifying potential endocrine-disrupting chemicals within 3 years. To aid in the identification of methods suitable for this purpose, the U.S. EPA, the Chemical Manufacturers Association, and the World Wildlife Fund sponsored...
Authors
Gerald T. Ankley, Ellen Mihaich, Ralph G. Stahl, Donald E. Tillitt, Theo Colborn, Suzzanne McMaster, Ron Miller, John Bantle, Pamela Campbell, Nancy Denslow, Richard L. Dickerson, Leroy C. Folmar, Michael Fry, John P. Giesy, L. Earl Gray, Patrick Guiney, Thomas Hutchinson, Sean W. Kennedy, Vincent Kramer, Gerald A. LeBlanc, Monte Mayes, Alison Nimrod, Reynaldo Patino, Richard Peterson, Richard Purdy, Robert Ringer, Peter C. Thomas, Les Touart, Glen Van Der Kraak, Tim Zacharewski