Reynaldo Patino, PhD (Former Employee)
Science and Products
Filter Total Items: 56
Effects of cadmium, estradiol-17beta and their interaction on gonadal condition and metamorphosis of male and female African clawed frog, Xenopus laevis
To assess interaction effects between cadmium (Cd, a putative xenoestrogen) and estradiol-17beta (E(2)) on sex differentiation and metamorphosis, Xenopus laevis were exposed to solvent-control (0.005% ethanol), Cd (10microgL(-1)), E(2) (1microgL(-1)), or Cd and E(2) (Cd+E(2)) in FETAX medium from fertilization to 75d postfertilization. Each treatment was applied to four aquaria, each...
Authors
Bibek Sharma, Reynaldo Patiño
Sublethal effects of catch-and-release fishing: measuring capture stress, fish impairment, and predation risk using a condition index
The sublethal effects of simulated capture of red snapper (Lutjanus campechanus) were analysed using physiological responses, condition indexing, and performance variables. Simulated catch-and-release fishing included combinations of depth of capture and thermocline exposure reflective of environmental conditions experienced in the Gulf of Mexico. Frequency of occurrence of barotrauma...
Authors
Matthew D. Campbell, Reynaldo Patiño, J.M. Tolan, R. E. Strauss, S. Diamond
Sources and distribution of organic compounds using passive samplers in Lake Mead National Recreation Area, Nevada and Arizona, and their implications for potential effects on aquatic biota.
Th e delineation of lateral and vertical gradients of organic contaminants in lakes is hampered by low concentrations and nondetection of many organic compounds in water. Passive samplers (semipermeable membrane devices [SPMDs] and polar organic chemical integrative samplers [POCIS]) are well suited for assessing gradients because they can detect synthetic organic compounds (SOCs) at pg...
Authors
Michael R. Rosen, David A. Alvarez, Steven L. Goodbred, Thomas J. Leiker, Reynaldo Patiño
Effects of cadmium on growth, metamorphosis and gonadal sex differentiation in tadpoles of the African clawed frog, Xenopus laevis
Xenopus laevis larvae were exposed to cadmium (Cd) at 0, 1, 8. 85 or 860 mu g L(-1) in FETAX medium from 0 to 86 d postfertilization. Premetamorphic tadpoles were sampled on day 3 1; pre and prometamorphic tadpoles on day 49; and frogs (NF stage 66) between days 50 and 86. Survival, snout-vent length (SVL), tail length, total length, hindlimb length (HLL), initiation of metamorphic...
Authors
Bibek Sharma, Reynaldo Patiño
Interaction of xenobiotics with estrogen receptors α and β and a putative plasma sex hormone-binding globulin from channel catfish (Ictalurus punctatus)
Estrogens are important regulators of physiological functions. Although environmental contaminants (xenoestrogens) which interfere with estrogen signaling are of increasing concern, there is only limited information about their ability to interact with estrogen-binding proteins (SHBG) or receptors (ER). Recombinant ER?? and ?? were obtained after transient transfection of COS-7 cells...
Authors
William L. Gale, Reynaldo Patiño, Alec G. Maule
Phylogenetic sequence analysis, recombinant expression, and tissue distribution of a channel catfish estrogen receptor beta
An estrogen receptor β (ERβ) cDNA fragment was amplified by RT-PCR of total RNAextracted from liver and ovary of immature channel catfish. This cDNA fragment was used to screen an ovarian cDNA library made from an immature female fish. A clone was obtained that contained an open reading frame encoding a 575-amino-acid protein with a deduced molecular weight of 63.9 kDa. Maximum parsimony...
Authors
Zhenfang Xia, William L. Gale, Xiaotian Chang, David Langenau, Reynaldo Patiño, Alec G. Maule, Llewellyn D. Densmore
Novel transcripts of the estrogen receptor α gene in channel catfish
Complementary DNA libraries from liver and ovary of an immature female channel catfish were screened with a homologous ERα cDNA probe. The hepatic library yielded two new channel catfish ER cDNAs that encode N-terminal ERα variants of different sizes. Relative to the catfish ERα (medium size; 581 residues) previously reported, these new cDNAs encode Long-ERα (36 residues longer) and...
Authors
Reynaldo Patiño, Zhenfang Xia, William L. Gale, Chunfa Wu, Alec G. Maule, Xiaotian Chang
Overview of a workshop on screening methods for detecting potential (anti-) estrogenic/androgenic chemicals in wildlife
The U.S. Congress has passed legislation requiring the U.S. Environmental Protection Agency (U.S. EPA) to develop, validate, and implement screening tests for identifying potential endocrine-disrupting chemicals within 3 years. To aid in the identification of methods suitable for this purpose, the U.S. EPA, the Chemical Manufacturers Association, and the World Wildlife Fund sponsored...
Authors
Gerald T. Ankley, Ellen Mihaich, Ralph G. Stahl, Donald E. Tillitt, Theo Colborn, Suzzanne McMaster, Ron Miller, John Bantle, Pamela Campbell, Nancy Denslow, Richard L. Dickerson, Leroy C. Folmar, Michael Fry, John P. Giesy, L. Earl Gray, Patrick Guiney, Thomas Hutchinson, Sean W. Kennedy, Vincent Kramer, Gerald A. LeBlanc, Monte Mayes, Alison Nimrod, Reynaldo Patiño, Richard Peterson, Richard Purdy, Robert Ringer, Peter C. Thomas, Les Touart, Glen Van Der Kraak, Tim Zacharewski
Science and Products
Filter Total Items: 56
Effects of cadmium, estradiol-17beta and their interaction on gonadal condition and metamorphosis of male and female African clawed frog, Xenopus laevis
To assess interaction effects between cadmium (Cd, a putative xenoestrogen) and estradiol-17beta (E(2)) on sex differentiation and metamorphosis, Xenopus laevis were exposed to solvent-control (0.005% ethanol), Cd (10microgL(-1)), E(2) (1microgL(-1)), or Cd and E(2) (Cd+E(2)) in FETAX medium from fertilization to 75d postfertilization. Each treatment was applied to four aquaria, each...
Authors
Bibek Sharma, Reynaldo Patiño
Sublethal effects of catch-and-release fishing: measuring capture stress, fish impairment, and predation risk using a condition index
The sublethal effects of simulated capture of red snapper (Lutjanus campechanus) were analysed using physiological responses, condition indexing, and performance variables. Simulated catch-and-release fishing included combinations of depth of capture and thermocline exposure reflective of environmental conditions experienced in the Gulf of Mexico. Frequency of occurrence of barotrauma...
Authors
Matthew D. Campbell, Reynaldo Patiño, J.M. Tolan, R. E. Strauss, S. Diamond
Sources and distribution of organic compounds using passive samplers in Lake Mead National Recreation Area, Nevada and Arizona, and their implications for potential effects on aquatic biota.
Th e delineation of lateral and vertical gradients of organic contaminants in lakes is hampered by low concentrations and nondetection of many organic compounds in water. Passive samplers (semipermeable membrane devices [SPMDs] and polar organic chemical integrative samplers [POCIS]) are well suited for assessing gradients because they can detect synthetic organic compounds (SOCs) at pg...
Authors
Michael R. Rosen, David A. Alvarez, Steven L. Goodbred, Thomas J. Leiker, Reynaldo Patiño
Effects of cadmium on growth, metamorphosis and gonadal sex differentiation in tadpoles of the African clawed frog, Xenopus laevis
Xenopus laevis larvae were exposed to cadmium (Cd) at 0, 1, 8. 85 or 860 mu g L(-1) in FETAX medium from 0 to 86 d postfertilization. Premetamorphic tadpoles were sampled on day 3 1; pre and prometamorphic tadpoles on day 49; and frogs (NF stage 66) between days 50 and 86. Survival, snout-vent length (SVL), tail length, total length, hindlimb length (HLL), initiation of metamorphic...
Authors
Bibek Sharma, Reynaldo Patiño
Interaction of xenobiotics with estrogen receptors α and β and a putative plasma sex hormone-binding globulin from channel catfish (Ictalurus punctatus)
Estrogens are important regulators of physiological functions. Although environmental contaminants (xenoestrogens) which interfere with estrogen signaling are of increasing concern, there is only limited information about their ability to interact with estrogen-binding proteins (SHBG) or receptors (ER). Recombinant ER?? and ?? were obtained after transient transfection of COS-7 cells...
Authors
William L. Gale, Reynaldo Patiño, Alec G. Maule
Phylogenetic sequence analysis, recombinant expression, and tissue distribution of a channel catfish estrogen receptor beta
An estrogen receptor β (ERβ) cDNA fragment was amplified by RT-PCR of total RNAextracted from liver and ovary of immature channel catfish. This cDNA fragment was used to screen an ovarian cDNA library made from an immature female fish. A clone was obtained that contained an open reading frame encoding a 575-amino-acid protein with a deduced molecular weight of 63.9 kDa. Maximum parsimony...
Authors
Zhenfang Xia, William L. Gale, Xiaotian Chang, David Langenau, Reynaldo Patiño, Alec G. Maule, Llewellyn D. Densmore
Novel transcripts of the estrogen receptor α gene in channel catfish
Complementary DNA libraries from liver and ovary of an immature female channel catfish were screened with a homologous ERα cDNA probe. The hepatic library yielded two new channel catfish ER cDNAs that encode N-terminal ERα variants of different sizes. Relative to the catfish ERα (medium size; 581 residues) previously reported, these new cDNAs encode Long-ERα (36 residues longer) and...
Authors
Reynaldo Patiño, Zhenfang Xia, William L. Gale, Chunfa Wu, Alec G. Maule, Xiaotian Chang
Overview of a workshop on screening methods for detecting potential (anti-) estrogenic/androgenic chemicals in wildlife
The U.S. Congress has passed legislation requiring the U.S. Environmental Protection Agency (U.S. EPA) to develop, validate, and implement screening tests for identifying potential endocrine-disrupting chemicals within 3 years. To aid in the identification of methods suitable for this purpose, the U.S. EPA, the Chemical Manufacturers Association, and the World Wildlife Fund sponsored...
Authors
Gerald T. Ankley, Ellen Mihaich, Ralph G. Stahl, Donald E. Tillitt, Theo Colborn, Suzzanne McMaster, Ron Miller, John Bantle, Pamela Campbell, Nancy Denslow, Richard L. Dickerson, Leroy C. Folmar, Michael Fry, John P. Giesy, L. Earl Gray, Patrick Guiney, Thomas Hutchinson, Sean W. Kennedy, Vincent Kramer, Gerald A. LeBlanc, Monte Mayes, Alison Nimrod, Reynaldo Patiño, Richard Peterson, Richard Purdy, Robert Ringer, Peter C. Thomas, Les Touart, Glen Van Der Kraak, Tim Zacharewski