Research Spotlight: Precise Eggshell Thickness Measurements To Inform Bird Ecology And Mercury Monitoring

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In a study published in the journal Ecology and Evolution, USGS biologists provide precise eggshell thickness measurements for 12 species of birds that breed in the San Francisco Bay Estuary.

A fluffy brown chick stands on a rocky surface next to a yellowish egg mottled with brown spots

A Forster's tern chick near an egg near San Francisco Bay.

(Credit: Carley Schacter, USGS Western Ecological Research Center. Public domain.)

Eggshell thickness is important for physiological, ecological, and ecotoxicological studies of birds. Prior studies have found that eggshell thickness is related to gas exchange, contaminant exposure, embryonic development, and egg survival. Eggshell thickness is also one of several key variables used to calculate mercury concentrations in bird eggs, making accurate and precise measurements critical to mercury monitoring efforts. However, the role of eggshell thickness in ecophysiology can vary among species, and empirical eggshell thickness measurements are limited for many species and regions.

USGS biologists used a relatively new scientific instrument (a Hall effect thickness gauge) to measure eggshell thickness comprehensively and precisely for 12 San Francisco Bay species and compared the results to prior eggshell measurements. They also monitored nests to examine the relationship between eggshell thickness and egg development and survival in three species, American avocet (Recurvirostra americana), black-necked stilt (Himantopus mexicanus), and Forster's tern (Sterna forsteri).

Graph showing how eggshell thickness varies at different parts of a bird egg, with the thinnest parts at the ends

Mean eggshell thickness at 5 different equally spaced positions on Forster’s tern eggshells (n = 40). Eggshells studied were thicker at the egg equator than the ‘bottom’ and ‘top’ of the egg.

From Peterson et al. 2020.

The biologists measured eggshell thickness for species representing a range of female body masses. One of the smallest species of birds in this study, California least tern (Sternula antillarum browni), had the thinnest eggshells in this study. The largest species in this study, double crested cormorant (Phalcrocorax auratus albociliatus), had the thickest eggshells. Eggshell thickness varied not only among species and individual birds but also within individual eggs: the eggshells studied were generally 5% thicker at the central portion of the egg (egg equator) than the ‘bottom’ (sharp egg pole) and ‘top’ (blunt egg pole) of the egg, highlighting the importance of using standardized eggshell measurement methods. The study found no relationship between eggshell thickness and incubation age or total mercury contamination of the egg content.

USGS biologists previously showed that accurately calculating contaminant concentrations in bird eggs relies on accounting for eggshell thickness. Specifically, incorporating eggshell thickness results in fresh wet weight egg contaminant concentrations that are 6-13% more accurate than when the eggshell is ignored. These eggshell thickness measurements provide current, precise data that can be incorporated into calculations of mercury concentrations in locally breeding waterbirds, helping managers better monitor wildlife and ecosystem health.

 

Management Implications

Three whitish eggs with brown spots in the middle of a nest of brown twigs and leaves

A Forster's tern nest with eggs near San Francisco Bay.

(Public domain.)

  • Contaminant monitoring programs can use new eggshell thickness measurements to more accurately estimate egg content concentrations.
  • Newer technology (a Hall effect thickness gauge) provides a more precise and repeatable thickness measurement than analog micrometers that have been used in many previous studies.
  • Variable thickness measured at different points of the eggshell highlight the importance of using standardized eggshell measurement methods.

 

This spotlight refers to the following publication and data release:

Peterson, SP, JT Ackerman, MP Herzog, MS Toney, B Cooney and CA Hartman. 2020. Avian eggshell thickness in relation to egg morphometrics, embryonic development, and mercury contamination. Ecology and Evolution. https://doi.org/10.1002/ece3.6570

Peterson, S.H., and Ackerman, J.T., 2020, Avian eggshell thickness for 12 species in the western U.S. from 2014 to 2018: U.S. Geological Survey data release, https://doi.org/10.5066/P981OW6T.

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Date published: September 1, 2020

Avian eggshell thickness for 12 species in the western U.S. from 2014 to 2018

Eggshell thickness was measured at the egg equator and poles (sharp pole and blunt pole) for 12 avian species. Eggshell thickness was related to egg morphometrics (egg length and width), embryonic development, egg status, and mercury contamination. Individual eggshells each have up to 3 thickness measurements, linked by the eggshell identification. Each measurement has the corresp