Evaluation Of Rapid Viability-Polymerase Chain Reaction (RV-PCR) For Bacillus Globigii Spores In Water Matrices

Rapid water-quality tests for microorganisms are needed to quickly ensure that water is safe for consumption. This study will test a rapid-viability, quantitative polymerase chain reaction (RV-PCR) method for the detection of Bacillus spores. The method will be tested in large-volume drinking water samples that are concentrated by ultrafiltration, as well as in water that has been disinfected with chlorine. Results from the rapid viability method will be compared to results from traditional bacteria-culturing methods. This study is in cooperation with the U.S. Environmental Protection Agency, National Homeland Security Research Center.