Ohio Microbiology Program - QAQC

Science Center Objects

LIST OF APPENDICES

 

General Instructions

A1. Instructions for quality control of deionized water

A2. Buffer preparation

A3. Storage and maintenance of bacteria control cultures

A4. Laboratory map

 

Forms

B1. Temperature log sheet

B2. Sample log sheet

B3 Analytical Services Request Form

B4. Media and buffer quality-control log sheet

B5. Expendable supplies request form

B6. QC log for Actinomycetes detection by double agar layer method

B7. QC log for aerobic endospores by membrane filtration

 

Culture Methods

C1. M-Endo method for total coliforms

C2. MI agar method for total coliforms and Escherichia coli

C3. Colilert Quanti-Tray and presence/absence methods for total coliforms and Escherichia coli

C4. mFC method for fecal coliforms

C5. Modified mTEC method for Escherichia coli

C6. Colilert Quanti-Tray sediment method for Escherichia coli

C7. mEI method for enterococci

C8. Verification of enterococci colonies from mEI

C9. Enterolert Quanti-Tray for enterococci

C10. mCP agar method for Clostridium perfringens

C11. Indicator bacteria quality-control procedures

C12. Coliphage detection by USEPA Method 1602, single-agar layer method

C13. Coliphage detection by USEPA Method 1601, Two-step enrichment method

C14. Detection of Actinomycetes in water

C15. Detection of aerobic endospores in water

Molecular methods (appendices are available upon request)

D1. Collection and initial processing of water samples for MST markers and Cyanobacteria

D1a. Bench sheet for filtration and extraction for MST and Cyanobacteria

D2. Collection and initial processing of solid samples for MST markers and Cyanobacteria

D2a. Field sheet for collection of known-source fecal samples

D3. DNA extraction and purification using the GeneRite DNA-EZ kit

D3a. Extraction batch bench sheet

D4. Detection of MST markers and Cyanobacteria by qPCR

D4a. qPCR bench sheets for MST assays

D4b. qPCR bench sheets for Cyanobacteria DNA

D4c. RT-qPCR bench sheets for Cyanobacteria RNA

D5. Development and quantification of plasmid positive controls

D5a. Plasmid-based positive control bench sheet

D6. qPCR data interpretation and file management

D7. RNA extraction and purification using the MoBio PowerPlant RNA Isolation Kit with DNase

D8. Limits of blank, detection, and quantification for qPCR and qRT-PCR analyses

 

Enteric virus methods (appendices are available upon request)

E1. Virus elution and organic flocculation

E1a. Virus processing form

E1b. Virus qPCR batch sheet

E2. Molecular concentration and extraction

E2a.  Foam elution and concentration from dead-end ultrafiltration

E3. Reverse transcription and qPCR protocol for viruses

E3a. qPCR and RT-qPCR benchsheets for viruses

E4. Total culturable virus assay

E4a. Total culturable virus and viral titering benchsheet

E5. Collection and initial processing of water samples for human polyomarvirus

E5a. Human polyomavirus filtration benchsheet

E6.  Extraction for human polyomavirus samples

 

Cyanobacterial toxins

F1. Total microcystins - ADDA by ELISA

F1a. ELISA benchsheet and plate map template