Publications
USGS divers in the Elwha River
WFRC zebrafish laboratory
Processing a Lost River sucker
Below is a list of available WFRC peer reviewed and published science.
Filter Total Items: 2517
Washington Department of Natural Resources herring study 2000: Health of Puget Sound herring stocks Washington Department of Natural Resources herring study 2000: Health of Puget Sound herring stocks
Abstract not available
Authors
P.K. Hershberger, K. M. Kocan
Climate regimes and water temperature changes in the Columbia River: bioenergetic implications for predators of juvenile salmon Climate regimes and water temperature changes in the Columbia River: bioenergetic implications for predators of juvenile salmon
We examined how climatic regime shifts may have affected predation rates on juvenile Pacific salmonids (Oncorhynchus spp.) by northern squawfish (Ptychocheilus oregonensis, also called northern pikeminnow), smallmouth bass (Micropterus dolomieu), and walleye (Stizostedion vitreum) in the Columbia River. During 1933-1996, oceanic, coastal, and freshwater indices of climate were highly...
Authors
J.H. Petersen, J.F. Kitchell
Antibody-producing cells correlated to body weight in juvenile chinook salmon (Oncorhynchus tshawytscha) acclimated to optimal and elevated temperatures Antibody-producing cells correlated to body weight in juvenile chinook salmon (Oncorhynchus tshawytscha) acclimated to optimal and elevated temperatures
The immune response of juvenile chinook salmon (Oncorhynchus tshawytscha) ranging in weight from approximately 10 to 55 g was compared when the fish were acclimated to either 13 or 21?? C. A haemolytic plaque assay was conducted to determine differences in the number of antibody-producing cells (APC) among fish of a similar age but different body weights. Regression analyses revealed...
Authors
L.N.M. Harrahy, C.B. Schreck, A.G. Maule
Survival of the North American strain of viral hemorrhagic septicemia virus (VHSV) in filtered seawater and seawater containing ovarian fluid, crude oil and serum-enriched culture medium Survival of the North American strain of viral hemorrhagic septicemia virus (VHSV) in filtered seawater and seawater containing ovarian fluid, crude oil and serum-enriched culture medium
The North American strain of viral hemorrhagic septicemia virus (NA-VHSV) could be recovered for up to 40 h in natural filtered seawater (27 ppt) with a 50% loss of infectivity after approximately 10 h at 15°C. Addition of 10 ppb North Slope crude oil to the seawater had no effect on virus survival. However, when various concentrations of teleost ovarian fluid were added to seawater...
Authors
R. M. Kocan, P.K. Hershberger, N.E. Elder
Fish DNA vaccine against infectious hematopoietic necrosis virus: efficacy of various routes of immunization Fish DNA vaccine against infectious hematopoietic necrosis virus: efficacy of various routes of immunization
The DNA vaccine, pIHNVw-G, contains the gene for the glycoprotein (G) of the rhabdovirus infectious hematopoietic necrosis virus (IHNV), a major pathogen of salmon and trout. The relative efficacy of various routes of immunisation with pIHNVw-G was evaluated using 1.8 g rainbow trout fry vaccinated via intramuscular injection, scarification of the skin, intraperitoneal injection...
Authors
Serge Corbeil, Gael Kurath, Scott E. LaPatra
Understanding aquatic animal virus survival and trafficking and its role in risk assessment Understanding aquatic animal virus survival and trafficking and its role in risk assessment
The stability of infectious agents in different media and under different physical and chemical environments has been extensively studied for some viruses and virtually ignored for others. Gaps in our knowledge are due in part to difficulties in reproducing virus «life cycles» and determination if the agent is in fact inactive. Additionally, isolation of the agent under certain...
Authors
S. LaPatra, R. Troyer, W. Shewmaker, G. Jones, Gael Kurath
Nanogram quantities of a DNA vaccine protect rainbow trout fry against heterologous strains of infectious hematopoietic necrosis virus Nanogram quantities of a DNA vaccine protect rainbow trout fry against heterologous strains of infectious hematopoietic necrosis virus
The efficacy of a DNA vaccine containing the glycoprotein gene of infectious hematopoietic necrosis virus (IHNV), a rhabdovirus affecting trout and salmon, was investigated. The minimal dose of vaccine required, the protection against heterologous strains, and the titers of neutralizing antibodies produced were used to evaluate the potential of the vaccine as a control pharmaceutical...
Authors
S. Corbeil, S. E. LaPatra, Eric Anderson, G. Kurath
Phylogenetic sequence analysis, recombinant expression, and tissue distribution of a channel catfish estrogen receptor beta Phylogenetic sequence analysis, recombinant expression, and tissue distribution of a channel catfish estrogen receptor beta
An estrogen receptor β (ERβ) cDNA fragment was amplified by RT-PCR of total RNAextracted from liver and ovary of immature channel catfish. This cDNA fragment was used to screen an ovarian cDNA library made from an immature female fish. A clone was obtained that contained an open reading frame encoding a 575-amino-acid protein with a deduced molecular weight of 63.9 kDa. Maximum parsimony...
Authors
Zhenfang Xia, William L. Gale, Xiaotian Chang, David Langenau, Reynaldo Patino, Alec G. Maule, Llewellyn D. Densmore
Direct behavioral evidence that unique bile acids released by larval sea lamprey (Petromyzon marinus) function as a migratory pheromone Direct behavioral evidence that unique bile acids released by larval sea lamprey (Petromyzon marinus) function as a migratory pheromone
Four behavioral experiments conducted in both the laboratory and the field provide evidence that adult sea lamprey (Petromyzon marinus) select spawning rivers based on the odor of larvae that they contain and that bile acids released by the larvae are part of this pheromonal odor. First, when tested in a recirculating maze, migratory adult lamprey spent more time in water scented with...
Authors
Rickard Bjerselius, Weiming Li, John H. Teeter, James G. Seelye, Peter B. Johnson, Peter J. Maniak, Gerold C. Grant, Christine N. Polkinghorne, Peter W. Sorensen
Genetic analyses reveal unusually high diversity of infectious haematopoietic necrosis virus in rainbow trout aquaculture Genetic analyses reveal unusually high diversity of infectious haematopoietic necrosis virus in rainbow trout aquaculture
Infectious haematopoietic necrosis virus (IHNV) is the most significant virus pathogen of salmon and trout in North America. Previous studies have shown relatively low genetic diversity of IHNV within large geographical regions. In this study, the genetic heterogeneity of 84 IHNV isolates sampled from rainbow trout (Oncorhynchus mykiss) over a 20 year period at four aquaculture...
Authors
Ryan M. Troyer, Scott E. LaPatra, Gael Kurath
Identification of juvenile fall versus spring chinook salmon migrating through the lower Snake River based on body morphology Identification of juvenile fall versus spring chinook salmon migrating through the lower Snake River based on body morphology
We tested the use of body morphology to distinguish among subyearling fall-run, subyearling spring-run, and yearling spring-run smolts of chinook salmon Oncorhynchus tshawytscha at two lower Snake River dams during the summer emigration. Based on principal-components analysis, subyearling fall-run chinook salmon had smaller heads and eyes, deeper bodies, and shorter caudal peduncles than...
Authors
K.F. Tiffan, D.W. Rondorf, R.D. Garland, P.A. Verhey