Dust suppressants and soil stabilizer products are applied to unpaved roads worldwide to reduce dust production and stabilize road surfaces. Although these products may enter roadside surface water through runoff or leaching, little information is available on environmental fate or aquatic toxicity. The data reported here include the acute toxicity of 27 dust suppressant/soil stabilizer products to juvenile rainbow trout (Oncorhynchus mykiss) and the acute toxicity of selected products to fatmucket mussels (Lampsilis siliquoidea), virile crayfish (Faxonius virilis), pond snails (Lymnaea stagnalis), and larval gray treefrogs (Dryophytes versicolor). Selected products were also tested after exposure to simulated weathering under UV radiation to evaluate the potential for photoenhanced toxicity. Toxicity data (based on average responses of organisms to nominal exposure concentrations of products) and average water quality data have been reported in a journal publication. In this data release we report individual biological and water quality measurements from each toxicity test.