The files in this data release are the raw 16S rRNA gene amplicon DNA sequence files from 28 samples of deep-sea corals Acanthogorgia aspera, Acanthogorgia spissa, Desmophyllum dianthus, and Lophelia pertusa, as well as an extraction blank for the Qiagen PowerBiofilm kit used for DNA extraction. The samples were collected during four research cruises from various locations in the Gulf of Mexico and the Atlantic Ocean off the east coast of the United States in deep-sea coral ecosystems from 2013 to 2019 for microbial analysis.
Extraction of DNA from the samples occurred at the USGS Coral Microbial Ecology Laboratory in St. Petersburg, FL, using Qiagen's DNeasy PowerBiofilm kit. PCR amplification and sequencing were performed by RTSF Genomics Core at Michigan State University using primers 341F/806R to target the V3-V4 region of the 16S rRNA gene on a MiSeq sequencing system with v2 chemistry to obtain paired-end 250-bp reads. The raw data files associated with this data release have also been submitted to the NCBI Sequence Read Archive under BioProject number PRJNA699458. For more information, please see the README file and metadata files.
Kellogg, C.A. and Pratte, Z.A., 2021, Unexpected diversity of Endozoicomonas in deep-sea corals: Marine Ecology Progress Series, v.673, article 13844, 15 p., https://doi.org/10.3354/meps13844.