Detection of Seagrass Pathogens using Environmental DNA (eDNA), North Pacific, 2016-Present
This data set is comprised of one table with sampling information and NCBI BioProject accession numbers for sequence information of this amplicon-based study targeting Labyrinthula, Phytophthora, and Halophytophthora of known pathogenic and non-pathogenic [to eelgrass (Zostera marina)] strains from eDNA samples. eDNA samples included water, sediment, and eelgrass from Notsuke Wan (Cove) Japan, Safety Sound, Izembek Lagoon, Port Moller, Chignik Lagoon, and Frederick Sound, Alaska and cloacal swabs from waterfowl hunted near Cold Bay Alaska. Replicate samples and multiple sampling dates of the same location were included. Highly conserved primers which could differentiate species of interest were developed for four portions of mtDNA genes (5.8S, 18S, ITS, and COI). The reference list and conserved primer sets to identify species present were developed using publicly available data https://www.ncbi.nlm.nih.gov/genbank/
Citation Information
Publication Year | 2021 |
---|---|
Title | Detection of Seagrass Pathogens using Environmental DNA (eDNA), North Pacific, 2016-Present |
DOI | 10.5066/P9T69I3V |
Authors | Damian M Menning, Barbara J Pierson, Sandra L Talbot |
Product Type | Data Release |
Record Source | USGS Digital Object Identifier Catalog |
USGS Organization | Alaska Science Center |
Related Content
Are migratory waterfowl vectors of seagrass pathogens?
Related Content
- Publications
Are migratory waterfowl vectors of seagrass pathogens?
Migratory waterfowl vector plant seeds and other tissues, but little attention has focused on the potential of avian vectoring of plant pathogens. Extensive meadows of eelgrass (Zostera marina) in southwest Alaska support hundreds of thousands of waterfowl during fall migration and may be susceptible to plant pathogens. We recovered DNA of organisms pathogenic to eelgrass from environmental sampAuthorsDamian M. Menning, David H. Ward, Sandy Wyllie-Echeverria, Kevin Sage, Megan C. Gravley, Hunter Gravley, Sandra L. Talbot - Connect