Microbial concentrations derived from laboratory experiments with hemodialyzers performed in Marshfield, Wisconsin

Laboratory experiments conducted in 2023 by the Laboratory for Infectious Disease and the Environment (LIDE) evaluated recovery of microorganisms by dead-end ultrafiltration using the Nipro Elisio™ hemodialyzer, with comparison to the Asahi Kasei Rexeed™ hemodialyzer. Experiments included 2 bacteria, 4 viruses, and 1 protozoan, including Campylobacter jejuni, Salmonella enterica serovar Typhimurium, adenovirus 41, vaccine-derived poliovirus Sabin 3, modified live porcine reproductive and respiratory syndrome virus type 2, serum-derived porcine circovirus 2, and Cryptosporidium parvum. Microorganisms were added to dechlorinated tap water from a groundwater source and were recovered by ultrafiltration using two filter types, and microbial gene targets were measured by quantitative polymerase chain reaction (qPCR). Collaborators with the U.S. Geological Survey included the U.S. Department of Agriculture-Agricultural Research Service, Swine Medicine Education Center of Iowa State University, and the College of Veterinary Medicine at Iowa State University.