Multi sequence file (.msf) file used to construct a 28S rRNA phylogenetic tree of species of Euryhelmis and other heterophyid trematodes. Sequences were generated from metacercariae samples at U.S. Geological Survey National Wildlife Health Center (NWHC) and compared for base pair similarities with publicly available sequences from GenBank using BLAST (https://blast.ncbi.nlm.nih.gov/Blast.cgi). Selection of GenBank sequences to include in the .msf file was based on the number of base pair similarities (94% or higher identity to this study’s sequences), 98–100% coverage AND stage of parasite from which the sequence was generated. An excel file listing all parasites and DNA from NWHC vouchered with the University of New Mexico, Museum of Southwestern Biology, Division of Parasitology along with GenBank Voucher numbers of deposited partial 28S rRNA, mitochondrial CO1 and 18S rRNA gene sequences.
