Quantitative polymerase chain reaction detection data for controlled DNA contamination experiments
August 20, 2021
This data shows the quantification cycle at which fluorescence signals crossed a threshold fluorescence for samples analyzed as part of controlled experiments to determine whether contaminating DNA is present in samples under varying experimental conditions.
Citation Information
Publication Year | 2021 |
---|---|
Title | Quantitative polymerase chain reaction detection data for controlled DNA contamination experiments |
DOI | 10.5066/P94UHEPJ |
Authors | Patrick R. Hutchins |
Product Type | Data Release |
Record Source | USGS Asset Identifier Service (AIS) |
USGS Organization | Northern Rocky Mountain Science Center (NOROCK) Headquarters |
Rights | This work is marked with CC0 1.0 Universal |
Related
Time to get real with qPCR controls: The frequency of sample contamination and the informative power of negative controls in environmental DNA studies Time to get real with qPCR controls: The frequency of sample contamination and the informative power of negative controls in environmental DNA studies
Environmental (e)DNA methods have enabled rapid, sensitive and specific inferences of taxa presence throughout diverse fields of ecological study. However, use of eDNA results for decision-making has been impeded by uncertainties associated with false positive tests putatively caused by sporadic or systemic contamination. Sporadic contamination is a process that is inconsistent across...
Authors
Patrick Ross Hutchins, Leah Nicole Simantel, Adam Sepulveda
Related
Time to get real with qPCR controls: The frequency of sample contamination and the informative power of negative controls in environmental DNA studies Time to get real with qPCR controls: The frequency of sample contamination and the informative power of negative controls in environmental DNA studies
Environmental (e)DNA methods have enabled rapid, sensitive and specific inferences of taxa presence throughout diverse fields of ecological study. However, use of eDNA results for decision-making has been impeded by uncertainties associated with false positive tests putatively caused by sporadic or systemic contamination. Sporadic contamination is a process that is inconsistent across...
Authors
Patrick Ross Hutchins, Leah Nicole Simantel, Adam Sepulveda